EDC3/YEL015W Summary Help

Standard Name EDC3 1
Systematic Name YEL015W
Alias DCP3 , LSM16
Feature Type ORF, Verified
Description Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; mediates decay of the RPS28B mRNA via binding to both Rps28Bp (or Rps28Ap) and the RPS28B mRNA; mediates decay of the YRA1 mRNA by a different, translation-independent mechanism; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress (2, 3, 4)
Name Description Enhancer of mRNA DeCapping 1, 2
Chromosomal Location
ChrV:126629 to 128284 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All EDC3 GO evidence and references
  View Computational GO annotations for EDC3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
126 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 35
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Biochemical Activity: 3
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 3
  • Protein-peptide: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 38

Genetic Interactions
  • Negative Genetic: 13
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 551
Molecular Weight (Da) 61,340
Isoelectric Point (pI) 6.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:126629 to 128284 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1656 126629..128284 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000741
References cited on this page View Complete Literature Guide for EDC3
1) Samanta MP and Liang S  (2003) Predicting protein functions from redundancies in large-scale protein interaction networks. Proc Natl Acad Sci U S A 100(22):12579-83
2) Kshirsagar M and Parker R  (2004) Identification of Edc3p as an enhancer of mRNA decapping in Saccharomyces cerevisiae. Genetics 166(2):729-39
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) He F, et al.  (2014) Yeast Edc3 Targets RPS28B mRNA for Decapping by Binding to a 3' Untranslated Region Decay-Inducing Regulatory Element. Mol Cell Biol 34(8):1438-51