VAC8/YEL013W Summary Help

Standard Name VAC8
Systematic Name YEL013W
Alias YEB3
Feature Type ORF, Verified
Description Phosphorylated and palmitoylated vacuolar membrane protein; interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions (1, 2, 3, 4)
Name Description VACuole related
Chromosomal Location
ChrV:128825 to 130561 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VAC8 GO evidence and references
  View Computational GO annotations for VAC8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
repressible
Resources
153 total interaction(s) for 105 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 10
  • Biochemical Activity: 3
  • Co-fractionation: 4
  • Co-localization: 2
  • PCA: 12
  • Reconstituted Complex: 4
  • Two-hybrid: 13

Genetic Interactions
  • Negative Genetic: 61
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 9
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 578
Molecular Weight (Da) 63,207
Isoelectric Point (pI) 4.86
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:128825 to 130561 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1737 128825..130561 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000739
References cited on this page View Complete Literature Guide for VAC8
1) Pan X, et al.  (2000) Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p. Mol Biol Cell 11(7):2445-57
2) Scott SV, et al.  (2000) Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. J Biol Chem 275(33):25840-9
3) Wang YX, et al.  (1998) Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole. J Cell Biol 140(5):1063-74
4) Subramanian K, et al.  (2006) Palmitoylation determines the function of Vac8 at the yeast vacuole. J Cell Sci 119(Pt 12):2477-85