Standard Name	VAC8
Systematic Name	YEL013W
Alias	YEB3
Feature Type	ORF, Verified
Description	Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions (1, 2, 3, 4)
Name Description	VACuole related

Chromosomal Location	ChrV:128825 to 130561 | ORF Map | GBrowse

	View Computational GO annotations for VAC8
Molecular Function
Manually curated	protein anchor (IMP, IPI)
Biological Process
Manually curated	CVT vesicle assembly (IMP) macroautophagy (IMP) nucleus-vacuole junction assembly (IMP) piecemeal microautophagy of nucleus (IMP) vacuole fusion, non-autophagic (IMP) vacuole inheritance (IMP)
Cellular Component
Manually curated	fungal-type vacuole membrane (IDA) Myo2p-Vac17p-Vac8p transport complex (IDA, IPI) nucleus-vacuole junction (IDA, IPI)

Classical genetics
null	autophagy: decreased metal resistance: increased prion formation: increased GFP-Osh1p accumulation: increased Cla4p distribution: abnormal Ape1p (API) modification: absent replicative lifespan: decreased vacuolar morphology: abnormal
Large-scale survey
null	auxotrophy cell cycle progression in G1 phase: increased duration competitive fitness: decreased endocytosis: decreased haploproficient heat sensitivity: increased Fus-Mid-GFP distribution: abnormal Pho8p (ALP) modification: decreased Cps1p (CPS) modification: decreased resistance to caffeine: decreased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to fluconazole: increased resistance to methyl methanesulfonate: increased resistance to sirolimus: decreased resistance to tunicamycin: decreased resistance to sodium selenide: decreased resistance to wortmannin: normal resistance to cycloheximide: increased sporulation: absent sporulation efficiency: decreased starvation resistance: decreased stress resistance: increased thermotolerance: decreased transposable element transposition: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	150 total interaction(s) for 104 unique genes/features.
Physical Interactions	Affinity Capture-MS: 11 Affinity Capture-RNA: 1 Affinity Capture-Western: 10 Biochemical Activity: 3 Co-fractionation: 4 Co-localization: 2 PCA: 12 Reconstituted Complex: 4 Two-hybrid: 13
Genetic Interactions	Negative Genetic: 61 Phenotypic Enhancement: 2 Phenotypic Suppression: 2 Positive Genetic: 8 Synthetic Growth Defect: 15 Synthetic Lethality: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:128825 to 130561 | ORF Map | GBrowse
Last Update	Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1737 128825..130561 1996-07-31 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000739