UBC8/YEL012W Summary Help

Standard Name UBC8 1
Systematic Name YEL012W
Alias GID3 2 , 3
Feature Type ORF, Verified
Description Ubiquitin-conjugating enzyme that regulates gluconeogenesis; negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro (1, 4, 5)
Name Description UBiquitin-Conjugating 1
Chromosomal Location
ChrV:131772 to 132551 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All UBC8 GO evidence and references
  View Computational GO annotations for UBC8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
86 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 64
  • Positive Genetic: 5
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 218
Molecular Weight (Da) 24,633
Isoelectric Point (pI) 3.91
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:131772 to 132551 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2000-12-01 | Sequence: 2000-12-01
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..5 131772..131776 2000-12-01 2000-12-01
Intron 6..128 131777..131899 2000-12-01 2000-12-01
CDS 129..780 131900..132551 2000-12-01 2000-12-01
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000738
References cited on this page View Complete Literature Guide for UBC8
1) Qin S, et al.  (1991) Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family. J Biol Chem 266(23):15549-54
2) Schule T, et al.  (2000) Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeast. EMBO J 19(10):2161-7
3) Hammerle M, et al.  (1998) Proteins of newly isolated mutants and the amino-terminal proline are essential for ubiquitin-proteasome-catalyzed catabolite degradation of fructose-1,6-bisphosphatase of Saccharomyces cerevisiae. J Biol Chem 273(39):25000-5
4) Kaiser P, et al.  (1994) A human ubiquitin-conjugating enzyme homologous to yeast UBC8. J Biol Chem 269(12):8797-802
5) Regelmann J, et al.  (2003) Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways. Mol Biol Cell 14(4):1652-63