WBP1/YEL002C Summary

Standard Name	WBP1 1
Systematic Name	YEL002C
Feature Type	ORF, Verified
Description	Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum (1, 2, 3 and see Summary Paragraph)
Name Description	Wheat germ agglutinin-Binding Protein 1

Chromosomal Location	ChrV:150014 to 148722 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All WBP1 GO evidence and references

	View Computational GO annotations for WBP1
Molecular Function
Manually curated	dolichyl-diphosphooligosaccharide-protein glycotransferase activity (IPI)
Biological Process
Manually curated	cell cycle (IDA) protein N-linked glycosylation (IPI)
Cellular Component
Manually curated	endoplasmic reticulum (IDA) nuclear envelope (IDA) oligosaccharyltransferase complex (IPI)

Mutant phenotypes All WBP1 Phenotype evidence and references

Classical genetics
conditional	apoptosis: increased heat sensitivity: increased Prc1p modification: decreased
repressible	Prc1p modification: decreased
unspecified	resistance to hygromycin B: decreased
Large-scale survey
null	inviable
overexpression	vegetative growth: decreased rate
reduction of function	competitive fitness: decreased resistance to methyl methanesulfonate: decreased
repressible	chromosome/plasmid maintenance: decreased
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All WBP1 Interaction evidence and references

	457 total interaction(s) for 276 unique genes/features.
Physical Interactions	Affinity Capture-MS: 18 Affinity Capture-RNA: 1 Affinity Capture-Western: 26 Co-purification: 6 PCA: 56 Protein-peptide: 3 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 3 Negative Genetic: 287 Phenotypic Suppression: 1 Positive Genetic: 44 Synthetic Growth Defect: 6 Synthetic Lethality: 3 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All WBP1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:150014 to 148722 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1293	150014..148722	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000728

SUMMARY PARAGRAPH for WBP1

During N-linked glycosylation of proteins, oligosaccharide chains are assembled on the carrier molecule dolichyl pyrophosphate in the following order: 2 molecules of N-acetylglucosamine (GlcNAc), 9 molecules of mannose, and 3 molecules of glucose. These 14-residue oligosaccharide cores are then transferred to asparagine residues on nascent polypeptide chains in the endoplasmic reticulum (ER). As proteins progress through the Golgi apparatus, the oligosaccharide cores are modified by trimming and extension to generate a diverse array of glycosylated proteins (reviewed in 4, 5).

The oligosaccharyl transferase complex (OST complex) (EC 2.4.1.119) transfers 14-sugar branched oligosaccharides from dolichyl pyrophosphate to asparagine residues. The complex contains nine protein subunits: Ost1p, Ost2p, Ost3p, Ost4p, Ost5p, Ost6p, Stt3p, Swp1p, and Wbp1p, all of which are integral membrane proteins of the ER. The OST complex interacts with the Sec61p pore complex (6) involved in protein import into the ER.

Wbp1p is the beta subunit of the OST complex, one of the original six subunits purified (7). WBP1 is essential (1, 2), but conditional mutants have decreased transferase activity (2, 3). Wbp1p is homologous to mammalian OST48 (OMIM) (8).

Last updated: 2005-06-27

References cited on this page View Complete Literature Guide for WBP1

1)	te Heesen S, et al. (1991) An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene. Eur J Cell Biol 56(1):8-18
2)	te Heesen S, et al. (1992) The yeast WBP1 is essential for oligosaccharyl transferase activity in vivo and in vitro. EMBO J 11(6):2071-5
3)	te Heesen S, et al. (1993) Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity. EMBO J 12(1):279-84
4)	Herscovics A and Orlean P (1993) Glycoprotein biosynthesis in yeast. FASEB J 7(6):540-50
5)	Burda P and Aebi M (1999) The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta 1426(2):239-57
6)	Chavan M, et al. (2005) Subunits of the translocon interact with components of the oligosaccharyl transferase complex. J Biol Chem 280(24):22917-24
7)	Kelleher DJ and Gilmore R (1994) The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides. J Biol Chem 269(17):12908-17
8)	Silberstein S, et al. (1992) The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1. J Biol Chem 267(33):23658-63