MSH3/YCR092C Summary Help

Standard Name MSH3
Systematic Name YCR092C
Feature Type ORF, Verified
Description Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability (1, 2 and see Summary Paragraph)
Name Description MutS Homolog
Chromosomal Location
ChrIII:279820 to 276764 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MSH3 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
70 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 2
  • Co-purification: 2
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 8
  • Phenotypic Enhancement: 25
  • Phenotypic Suppression: 2
  • Positive Genetic: 2
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 1,018
Molecular Weight (Da) 116,533
Isoelectric Point (pI) 7.14
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIII:279820 to 276764 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2007-09-06
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3057 279820..276764 2011-02-03 2007-09-06
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000688

Msh3p functions in conjunction with Msh2p and Msh6p in the mismatch repair (MMR) system, which plays an important role in repairing single-base mismatches and mispaired regions due to small insertions or deletions (3). Genetic and biochemical results suggest that the Msh2p-Msh3p heterodimer has a different substrate specificity than the Msh2p-Msh6p heterodimer (4, 5, 6). The Msh2p-Msh3p heterodimer has a higher affinity for insertion-deletion loops larger than a single nucleotide and does not bind single-base mismatches with high affinity (5, 6, 7).

In addition to their role in mismatch repair, MSH2 and MSH3 are required for the removal of non-homologous DNA and regulation of heteroduplex formation during mitotic and meiotic recombination (1, 8, 9, 10). The Msh2p-Msh3p heterodimer binds double-strand/single-strand DNA junctions, possibly acting as a scaffold to recruit additional DNA repair proteins such as Mlh1p-Pms1p heterodimer or Rad1p-Rad10p heterodimer (11, 6).

Msh3p is one of six E. coli MutS homologs in S. cerevisiae but is more similar to the mouse Rep-3 protein (12). Mutations in the H. sapiens MSH3 protein have been identified in an endometrial carcinoma (OMIM) and in an endometrial carcinoma cell line (13).

Last updated: 2006-09-05 Contact SGD

References cited on this page View Complete Literature Guide for MSH3
1) Sugawara N, et al.  (1997) Role of Saccharomyces cerevisiae Msh2 and Msh3 repair proteins in double-strand break-induced recombination. Proc Natl Acad Sci U S A 94(17):9214-9
2) Clark AB, et al.  (2000) Functional interaction of proliferating cell nuclear antigen with MSH2-MSH6 and MSH2-MSH3 complexes. J Biol Chem 275(47):36498-501
3) Harfe BD and Jinks-Robertson S  (2000) Mismatch repair proteins and mitotic genome stability. Mutat Res 451(1-2):151-67
4) Johnson RE, et al.  (1996) Requirement of the yeast MSH3 and MSH6 genes for MSH2-dependent genomic stability. J Biol Chem 271(13):7285-8
5) Alani E  (1996) The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs. Mol Cell Biol 16(10):5604-15
6) Surtees JA and Alani E  (2006) Mismatch repair factor MSH2-MSH3 binds and alters the conformation of branched DNA structures predicted to form during genetic recombination. J Mol Biol 360(3):523-36
7) Habraken Y, et al.  (1996) Binding of insertion/deletion DNA mismatches by the heterodimer of yeast mismatch repair proteins MSH2 and MSH3. Curr Biol 6(9):1185-7
8) Studamire B, et al.  (1999) Separation-of-function mutations in Saccharomyces cerevisiae MSH2 that confer mismatch repair defects but do not affect nonhomologous-tail removal during recombination. Mol Cell Biol 19(11):7558-67
9) Chen W and Jinks-Robertson S  (1998) Mismatch repair proteins regulate heteroduplex formation during mitotic recombination in yeast. Mol Cell Biol 18(11):6525-37
10) Stone JE and Petes TD  (2006) Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae. Genetics 173(3):1223-39
11) Habraken Y, et al.  (1997) Enhancement of MSH2-MSH3-mediated mismatch recognition by the yeast MLH1-PMS1 complex. Curr Biol 7(10):790-3
12) New L, et al.  (1993) The yeast gene MSH3 defines a new class of eukaryotic MutS homologues. Mol Gen Genet 239(1-2):97-108
13) Risinger JI, et al.  (1996) Mutation of MSH3 in endometrial cancer and evidence for its functional role in heteroduplex repair. Nat Genet 14(1):102-5