PAT1/YCR077C Summary Help

Standard Name PAT1 1
Systematic Name YCR077C
Alias MRT1
Feature Type ORF, Verified
Description Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation (1, 2, 3, 4, 5, 6, 7)
Name Description Protein Associated with Topoisomerase II 1
Chromosomal Location
ChrIII:252628 to 250238 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PAT1 GO evidence and references
  View Computational GO annotations for PAT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
1184 total interaction(s) for 794 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 83
  • Affinity Capture-RNA: 372
  • Affinity Capture-Western: 20
  • Biochemical Activity: 4
  • Co-purification: 4
  • PCA: 6
  • Protein-RNA: 3
  • Reconstituted Complex: 8
  • Two-hybrid: 44

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 4
  • Negative Genetic: 434
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 7
  • Positive Genetic: 110
  • Synthetic Growth Defect: 46
  • Synthetic Lethality: 27
  • Synthetic Rescue: 8

Expression Summary
Length (a.a.) 796
Molecular Weight (Da) 88,511
Isoelectric Point (pI) 7.45
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIII:252628 to 250238 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
This feature contains embedded feature(s): YCRX15W
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2391 252628..250238 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000673
References cited on this page View Complete Literature Guide for PAT1
1) Wang X, et al.  (1996) Pat1: a topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiae. Nucleic Acids Res 24(23):4791-7
2) Wang X, et al.  (1999) The topoisomerase II-associated protein, Pat1p, is required for maintenance of rDNA locus stability in Saccharomyces cerevisiae. Mol Gen Genet 261(4-5):831-40
3) Bonnerot C, et al.  (2000) The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p. Mol Cell Biol 20(16):5939-46
4) Noueiry AO, et al.  (2003) Yeast Lsm1p-7p/Pat1p deadenylation-dependent mRNA-decapping factors are required for brome mosaic virus genomic RNA translation. Mol Cell Biol 23(12):4094-106
5) Ramachandran V, et al.  (2011) The cAMP-Dependent Protein Kinase Signaling Pathway Is a Key Regulator of P Body Foci Formation. Mol Cell 43(6):973-81
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Mitchell SF, et al.  (2013) Global analysis of yeast mRNPs. Nat Struct Mol Biol 20(1):127-33