PAT1/YCR077C Summary Help

Standard Name PAT1 1
Systematic Name YCR077C
Alias MRT1
Feature Type ORF, Verified
Description Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation (1, 2, 3, 4, 5, 6, 7)
Name Description Protein Associated with Topoisomerase II 1
Chromosomal Location
ChrIII:252628 to 250238 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PAT1 GO evidence and references
  View Computational GO annotations for PAT1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
1176 total interaction(s) for 793 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 83
  • Affinity Capture-RNA: 371
  • Affinity Capture-Western: 20
  • Biochemical Activity: 4
  • Co-purification: 4
  • PCA: 6
  • Protein-RNA: 3
  • Reconstituted Complex: 8
  • Two-hybrid: 40

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 4
  • Negative Genetic: 434
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 7
  • Positive Genetic: 110
  • Synthetic Growth Defect: 43
  • Synthetic Lethality: 27
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 796
Molecular Weight (Da) 88,511
Isoelectric Point (pI) 7.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:252628 to 250238 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
This feature contains embedded feature(s): YCRX15W
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2391 252628..250238 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000673
References cited on this page View Complete Literature Guide for PAT1
1) Wang X, et al.  (1996) Pat1: a topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiae. Nucleic Acids Res 24(23):4791-7
2) Wang X, et al.  (1999) The topoisomerase II-associated protein, Pat1p, is required for maintenance of rDNA locus stability in Saccharomyces cerevisiae. Mol Gen Genet 261(4-5):831-40
3) Bonnerot C, et al.  (2000) The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p. Mol Cell Biol 20(16):5939-46
4) Noueiry AO, et al.  (2003) Yeast Lsm1p-7p/Pat1p deadenylation-dependent mRNA-decapping factors are required for brome mosaic virus genomic RNA translation. Mol Cell Biol 23(12):4094-106
5) Ramachandran V, et al.  (2011) The cAMP-Dependent Protein Kinase Signaling Pathway Is a Key Regulator of P Body Foci Formation. Mol Cell 43(6):973-81
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Mitchell SF, et al.  (2013) Global analysis of yeast mRNPs. Nat Struct Mol Biol 20(1):127-33