RRP43/YCR035C Summary 
RRP43 BASIC INFORMATION
| Standard Name | RRP43 1 |
|---|---|
| Systematic Name | YCR035C |
| Feature Type | ORF, Verified |
| Description | Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8) (1, 2, 3, 4, 5 and see Summary Paragraph) 
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| Name Description | Ribosomal RNA Processing 1 |
| Interactions | RRP43 All interactions details and references |
|---|---|
| 101 total interaction(s) for 26 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
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| Primary SGDID | S000000631 |
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RRP43 RESOURCES
| SGD ORF map | GBrowse |
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for RRP43
SUMMARY PARAGRAPH for RRP43
The exosome complex possesses 3'-5' exonuclease and endoribonucleolytic activities that are essential for diverse ribonucleolytic processes in both the nucleus and the cytoplasm (1, 6, 7). The nuclear exosome is associated with the TRAMP complex and is involved in RNA catabolic processes including RNA surveillance (8, 9 and references therein), pre-mRNA turnover (10) and the production of mature 3' ends for snoRNAs, snRNAs and rRNAs (6, 11 and references therein). The cytoplasmic exosome is associated with Ski7p and the SKI complex and is involved in RNA catabolic processes that include both the routine turnover of normal mRNA (12) as well as the degradation of aberrant mRNAs (13 and references therein). The 10-subunit core exosome complex (Csl4p, Rrp4p, Rrp40p, Ski6p, Rrp42p, Rrp43p, Rrp45p, Rrp46p, Mtr3p, Dis3p) is the same in both locations, but the nuclear exosome contains an additional subunit (Rrp6p) and two additional accessory factors (Lrp1p, Mpp6p) (7).
Although the exosome was originally described as a "complex of exonucleases," with multiple subunits proposed to have RNase activity (1), later work has shown that this mechanism is unlikely in yeast. With the exception of Ski6p, none of the yeast subunits that show homology to E. coli RNase PH retain the active site residues seen in the bacterial or archael enzymes. Further research has also demonstrated that most, if not all, detectable enzymatic activity resides in the Dis3p and Rrp6p subunits (4, 5).
RRP43 encodes a core subunit of the exosome and has similarity to the RNase PH class of RNases (2, 1, 3, and references therein). Like most exosome components, Rrp43p is highly conserved among eukaryotes, including humans (hRrp43p (OIP2, EXOSC8)) (4 and references therein). RRP43 is an essential gene, but cells depleted for Rrp43p accumulate aberrant forms of rRNA (1, 14, 11) and temperature sensitive mutants have defects in rRNA processing and mRNA decay at the restrictive temperature 15.
Last updated: 2009-09-09
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RRP43]
| 1) | Mitchell P, et al. (1997) The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases. Cell 91(4):457-66 |
| 2) | Allmang C, et al. (1999) The yeast exosome and human PM-Scl are related complexes of 3' --> 5' exonucleases. Genes Dev 13(16):2148-58 |
| 3) | Synowsky SA, et al. (2006) Probing genuine strong interactions and post-translational modifications in the heterogeneous yeast exosome protein complex. Mol Cell Proteomics 5(9):1581-92 |
| 4) | Liu Q, et al. (2006) Reconstitution, activities, and structure of the eukaryotic RNA exosome. Cell 127(6):1223-37 |
| 5) | Dziembowski A, et al. (2007) A single subunit, Dis3, is essentially responsible for yeast exosome core activity. Nat Struct Mol Biol 14(1):15-22 |
| 6) | van Hoof A, et al. (2000) Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs. Mol Cell Biol 20(2):441-52 |
| 7) | Synowsky SA, et al. (2009) Comparative multiplexed mass spectrometric analyses of endogenously expressed yeast nuclear and cytoplasmic exosomes. J Mol Biol 385(4):1300-13 |
| 8) | Vanacova S, et al. (2005) A new yeast poly(A) polymerase complex involved in RNA quality control. PLoS Biol 3(6):e189 |
| 9) | LaCava J, et al. (2005) RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. Cell 121(5):713-24 |
| 10) | Bousquet-Antonelli C, et al. (2000) Identification of a regulated pathway for nuclear pre-mRNA turnover. Cell 102(6):765-75 |
| 11) | Allmang C, et al. (2000) Degradation of ribosomal RNA precursors by the exosome. Nucleic Acids Res 28(8):1684-91 |
| 12) | Anderson JS and Parker RP (1998) The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex. EMBO J 17(5):1497-506 |
| 13) | Schaeffer D, et al. (2008) Determining in vivo activity of the yeast cytoplasmic exosome. Methods Enzymol 448:227-39 |
| 14) | Zanchin NI and Goldfarb DS (1999) The exosome subunit Rrp43p is required for the efficient maturation of 5.8S, 18S and 25S rRNA. Nucleic Acids Res 27(5):1283-8 |
| 15) | Oliveira CC, et al. (2002) Temperature-sensitive mutants of the exosome subunit Rrp43p show a deficiency in mRNA degradation and no longer interact with the exosome. Nucleic Acids Res 30(19):4186-98 |
