RVS161/YCR009C Summary Help

Standard Name RVS161 1
Systematic Name YCR009C
Alias END6 2 , FUS7 3 , SPE161
Feature Type ORF, Verified
Description Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress (1, 2, 4, 5, 6, 7)
Name Description Reduced Viability on Starvation 1, 8
Chromosomal Location
ChrIII:131542 to 130745 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 1 cM
Gene Ontology Annotations All RVS161 GO evidence and references
  View Computational GO annotations for RVS161
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
reduction of function
Large-scale survey
673 total interaction(s) for 436 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 60
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 5
  • Co-fractionation: 2
  • Co-localization: 1
  • PCA: 6
  • Reconstituted Complex: 1
  • Two-hybrid: 37

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 5
  • Negative Genetic: 293
  • Phenotypic Enhancement: 15
  • Phenotypic Suppression: 8
  • Positive Genetic: 56
  • Synthetic Growth Defect: 94
  • Synthetic Lethality: 75
  • Synthetic Rescue: 9

Expression Summary
Length (a.a.) 265
Molecular Weight (Da) 30,250
Isoelectric Point (pI) 6.57
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIII:131542 to 130745 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 1 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..798 131542..130745 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000602
References cited on this page View Complete Literature Guide for RVS161
1) Crouzet M, et al.  (1991) Yeast mutant affected for viability upon nutrient starvation: characterization and cloning of the RVS161 gene. Yeast 7(7):727-43
2) Munn AL, et al.  (1995) end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae. Mol Biol Cell 6(12):1721-42
3) Gammie AE, et al.  (1998) Distinct morphological phenotypes of cell fusion mutants. Mol Biol Cell 9(6):1395-410
4) Navarro P, et al.  (1997) Protein-protein interaction between the RVS161 and RVS167 gene products of Saccharomyces cerevisiae. Biochim Biophys Acta 1343(2):187-92
5) Sivadon P, et al.  (1995) Actin cytoskeleton and budding pattern are altered in the yeast rvs161 mutant: the Rvs161 protein shares common domains with the brain protein amphiphysin. Mol Gen Genet 246(4):485-95
6) Brizzio V, et al.  (1998) Rvs161p interacts with Fus2p to promote cell fusion in Saccharomyces cerevisiae. J Cell Biol 141(3):567-84
7) Lombardi R and Riezman H  (2001) Rvs161p and Rvs167p, the two yeast amphiphysin homologs, function together in vivo. J Biol Chem 276(8):6016-22
8) Bauer F, et al.  (1993) Alteration of a yeast SH3 protein leads to conditional viability with defects in cytoskeletal and budding patterns. Mol Cell Biol 13(8):5070-84