CDC10/YCR002C Summary Help

CDC10 BASIC INFORMATION

Standard Name CDC10
Systematic Name YCR002C
Feature Type ORF, Verified
Description Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM (1 and see Summary Paragraph)
Name Description Cell Division Cycle 2
GO Annotations All CDC10 GO evidence and references
    View Computational GO annotations for CDC10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All CDC10 Phenotype details and references
Classical genetics
conditional
null
Large-scale survey
null
Interactions CDC10 All interactions details and references
117 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 38
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 16
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 5
  • FRET: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 10
Genetic Interactions
  • Dosage Rescue: 4
  • Phenotypic Enhancement: 27
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2
Sequence Information
ChrIII:118346 to 117378 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -.75 cM
Last Update Coordinates: 2006-01-12 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..969 118346..117378 2006-01-12 1997-01-28
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000595

CDC10 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for CDC10

SUMMARY PARAGRAPH for CDC10

CDC10 is a non-essential gene that encodes a septin (3, 4). Septins are a family of conserved proteins first identified in yeast and subsequently found in numerous other fungi and animals, including human, mouse, Drosophila, and C. elegans (reviewed in 3 and 4).

Septins are required for cytokinesis in many species (4); four yeast septin genes, CDC3, CDC10, CDC11, and CDC12, were identified through temperature-sensitive mutations that cause defects in cytokinesis (5). These yeast septins also function in axial bud site selection (6) and morphogenesis (7, 3, 4); they are required for the correct localization of several other proteins involved in cytokinesis, morphogenesis, and bud site selection (4, 6, 8, 9, 10). The yeast septins localize to a ring around the bud neck (6), and form a highly ordered filament structure (11). Mutations in CDC3, CDC10, CDC11 , or CDC12 disrupt the filaments, but cytokinesis can still proceed in the cdc10 deletion, suggesting that the filament structure is not necessary for this aspect of septin function (11). Cdc3p, Cdc10p, Cdc11p, and Cdc12p physically interact with three mitosis-specific protein kinases, Gin4p, Hsl1p and Kcc4p, which are involved in cell cycle progression (8, 9). The septins are required for the localization and activation of these protein kinases (8, 9).

All known septins contain consensus GTP-binding domains, and Drosophila septins hydrolyze GTP in vitro (3, 4). Septin GTPase activity has not been studied extensively in yeast (4).

Three more genes encoding septins, SHS1, SPR3, and SPR28, have been identified more recently and are less well characterized than the first four yeast septins (4).

Last updated: 1999-12-08

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CDC10]

1) Gladfelter AS, et al.  (2001) The septin cortex at the yeast mother-bud neck. Curr Opin Microbiol 4(6):681-9
2) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
3) Longtine MS, et al.  (1996) The septins: roles in cytokinesis and other processes. Curr Opin Cell Biol 8(1):106-19
4) Field CM and Kellogg D  (1999) Septins: cytoskeletal polymers or signalling GTPases? Trends Cell Biol 9(10):387-94
5) Hartwell LH  (1971) Genetic control of the cell division cycle in yeast. IV. Genes controlling bud emergence and cytokinesis. Exp Cell Res 69(2):265-76
6) Madden K and Snyder M  (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744
7) Cid VJ, et al.  (1998) Cell integrity and morphogenesis in a budding yeast septin mutant. Microbiology 144 ( Pt 12):3463-74
8) Carroll CW, et al.  (1998) The septins are required for the mitosis-specific activation of the Gin4 kinase. J Cell Biol 143(3):709-17
9) Barral Y, et al.  (1999) Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast. Genes Dev 13(2):176-87
10) Park HO, et al.  (1999) Localization of Bud2p, a GTPase-activating protein necessary for programming cell polarity in yeast to the presumptive bud site. Genes Dev 13(15):1912-7
11) Frazier JA, et al.  (1998) Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function. J Cell Biol 143(3):737-49