MRC1/YCL061C Summary

MRC1 BASIC INFORMATION

Standard Name	MRC1
Systematic Name	YCL061C
Alias	YCL060C
Feature Type	ORF, Verified
Description	S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres (1, 2, 3, 4)
Name Description	Mediator of the Replication Checkpoint 5

GO Annotations	All MRC1 GO evidence and references
	View Computational GO annotations for MRC1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	chromatin silencing at silent mating-type cassette (IGI, IMP) chromatin silencing at telomere (IGI, IMP) DNA repair (IGI, IMP) DNA replication (IMP) DNA replication checkpoint (IGI, IMP, IPI) intra-S DNA damage checkpoint (IMP) maintenance of DNA repeat elements (IMP) mitotic sister chromatid cohesion (IGI, IMP) replication fork protection (IGI, IMP, IPI) telomere maintenance (IMP)
Cellular Component
Manually curated	nuclear chromosome (IPI) nuclear replication fork (IDA) replication fork protection complex (IDA)
High-throughput	nucleus (IDA)

Mutant Phenotype	All MRC1 Phenotype details and references
Classical genetics
null	cell cycle progression in S phase: increased duration colony sectoring: increased resistance to selenomethionine: increased resistance to St. John's Wort extract: decreased resistance to hydroxyurea: decreased resistance to benomyl: decreased transposable element transposition: increased
reduction of function	cell cycle progression in S phase: increased duration cell cycle progression through the G1/S phase transition: delayed
Large-scale survey
null	chromosome/plasmid maintenance: decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased resistance to St. John's Wort extract: decreased telomere length: decreased thermotolerance: decreased transposable element transposition: increased viable

Interactions	MRC1 All interactions details and references
	418 total interaction(s) for 175 unique genes/features.
Physical Interactions	Affinity Capture-MS: 11 Affinity Capture-Western: 17 Biochemical Activity: 3 Two-hybrid: 5
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Dosage Rescue: 9 Phenotypic Enhancement: 126 Phenotypic Suppression: 22 Synthetic Growth Defect: 103 Synthetic Lethality: 111 Synthetic Rescue: 9

Sequence Information

ChrIII:22106 to 18816 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2000-09-13 | Sequence: 2000-09-13

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3291	22106..18816	2000-09-13	2000-09-13

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000566

MRC1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for MRC1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MRC1]

1)	Osborn AJ and Elledge SJ (2003) Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53. Genes Dev 17(14):1755-67
2)	Katou Y, et al. (2003) S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature 424(6952):1078-83
3)	Tsolou A and Lydall D (2007) Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1. DNA Repair (Amst) 6(11):1607-1617
4)	Lou H, et al. (2008) Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint. Mol Cell 32(1):106-17
5)	Alcasabas AA, et al. (2001) Mrc1 transduces signals of DNA replication stress to activate Rad53. Nat Cell Biol 3(11):958-65

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