MRC1/YCL061C Summary

MRC1/YCL061C Summary

Standard Name	MRC1
Systematic Name	YCL061C
Alias	YCL060C
Feature Type	ORF, Verified
Description	S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres (1, 2, 3, 4)
Name Description	Mediator of the Replication Checkpoint 5

Chromosomal Location	ChrIII:22106 to 18816 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All MRC1 GO evidence and references

	View Computational GO annotations for MRC1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	chromatin silencing at silent mating-type cassette (IGI, IMP) chromatin silencing at telomere (IGI, IMP) DNA repair (IGI, IMP) DNA replication (IMP) DNA replication checkpoint (IGI, IMP, IPI) intra-S DNA damage checkpoint (IMP) maintenance of DNA repeat elements (IMP) mitotic sister chromatid cohesion (IGI, IMP) replication fork protection (IGI, IMP, IPI) telomere maintenance (IMP)
Cellular Component
Manually curated	nuclear chromosome (IPI) nuclear replication fork (IDA) replication fork protection complex (IDA)
High-throughput	nucleus (IDA)

Mutant phenotypes All MRC1 Phenotype evidence and references

Classical genetics
null	cell cycle progression in S phase: increased duration chromosome/plasmid maintenance: abnormal colony sectoring: increased Rad53p modification: increased Smc3p modification: decreased resistance to hydroxyurea: decreased resistance to 4-aminofolic acid: decreased resistance to St. John's Wort extract: decreased resistance to benomyl: decreased resistance to selenomethionine: increased transposable element transposition: increased
reduction of function	cell cycle progression in S phase: increased duration cell cycle progression through the G1/S phase transition: delayed
Large-scale survey
null	cell size: increased competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased resistance to elesclomol-Cu: decreased resistance to St. John's Wort extract: decreased resistance to streptomycin: decreased resistance to cycloheximide: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to bleomycin: decreased resistance to quinine: decreased resistance to tunicamycin: increased resistance to mycophenolic acid: increased resistance to fluconazole: increased resistance to cycloheximide: increased resistance to camptothecin: increased telomere length: decreased thermotolerance: decreased transposable element transposition: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MRC1 Interaction evidence and references

	696 total interaction(s) for 281 unique genes/features.
Physical Interactions	Affinity Capture-MS: 16 Affinity Capture-Western: 36 Biochemical Activity: 4 Reconstituted Complex: 1 Two-hybrid: 9
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 2 Dosage Rescue: 9 Negative Genetic: 276 Phenotypic Enhancement: 41 Phenotypic Suppression: 6 Positive Genetic: 36 Synthetic Growth Defect: 150 Synthetic Lethality: 93 Synthetic Rescue: 15
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MRC1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIII:22106 to 18816 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2000-09-13 | Sequence: 2000-09-13

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3291	22106..18816	2000-09-13	2000-09-13

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000566

References cited on this page View Complete Literature Guide for MRC1

1)	Osborn AJ and Elledge SJ (2003) Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53. Genes Dev 17(14):1755-67
2)	Katou Y, et al. (2003) S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature 424(6952):1078-83
3)	Tsolou A and Lydall D (2007) Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1. DNA Repair (Amst) 6(11):1607-1617
4)	Lou H, et al. (2008) Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint. Mol Cell 32(1):106-17
5)	Alcasabas AA, et al. (2001) Mrc1 transduces signals of DNA replication stress to activate Rad53. Nat Cell Biol 3(11):958-65