RNQ1/YCL028W Summary Help

Standard Name RNQ1 1
Systematic Name YCL028W
Feature Type ORF, Verified
Description [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate (1, 2 and see Summary Paragraph)
Also known as: [PIN(+)]
Name Description Rich in asparagine (N) and glutamine (Q) 1
Chromosomal Location
ChrIII:70150 to 71367 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RNQ1 GO evidence and references
  View Computational GO annotations for RNQ1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All RNQ1 Phenotype evidence and references
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
interactions All RNQ1 Interaction evidence and references
107 total interaction(s) for 83 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 24
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 32
  • Co-crystal Structure: 2
  • Protein-peptide: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 19
Genetic Interactions
  • Dosage Rescue: 14
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 6
Resources
Expression Summary
histogram
Resources
Protein Information All RNQ1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:70150 to 71367 | ORF Map | GBrowse
This feature contains embedded feature(s): YCLX06C
SGD ORF map
Last Update Coordinates: 2000-09-13 | Sequence: 2000-09-13
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1218 70150..71367 2000-09-13 2000-09-13
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000533
SUMMARY PARAGRAPH for RNQ1

The [PSI+] prion determinant causes nonsense suppressor phenotype due to a reduced function of the translation termination factor Sup35p (eRF3) polymerized into amyloid fibrils. Prion state of the Rnq1 protein, [PIN+], is required for the [PSI+] de novo generation, but not propagation. Yeast [psi-] [PIN+] cells overproducing Sup35p can exhibit nonsense suppression without generation of a stable [PSI+]. In such cells most of Sup35p is present in amyloid polymers, though remaining Sup35p monomer is sufficient for normal translation termination. Presence of these polymers strictly depends on [PIN+], suggesting that their maintenance relies on efficient generation de novo, rather than inheritance. Sup35p polymers contain Rnq1p, confirming that Rnq1p polymers seed Sup35p polymerization (3).

Last updated: 2005-03-15

References cited on this page View Complete Literature Guide for RNQ1
1) Sondheimer N and Lindquist S  (2000) Rnq1: an epigenetic modifier of protein function in yeast. Mol Cell 5(1):163-72
2) Derkatch IL, et al.  (2004) Effects of Q/N-rich, polyQ, and non-polyQ amyloids on the de novo formation of the [PSI+] prion in yeast and aggregation of Sup35 in vitro. Proc Natl Acad Sci U S A 101(35):12934-9
3) Salnikova AB, et al.  (2005) Nonsense suppression in yeast cells overproducing Sup35 (eRF3) is caused by its non-heritable amyloids. J Biol Chem 280(10):8808-12