STP22/YCL008C Summary Help

Standard Name STP22
Systematic Name YCL008C
Alias VPS23 1 , 2 , VPL15 2
Feature Type ORF, Verified
Description Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype (3, 4)
Name Description STerile Pseudoreversion 5
Chromosomal Location
ChrIII:106853 to 105696 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All STP22 GO evidence and references
  View Computational GO annotations for STP22
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All STP22 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
interactions All STP22 Interaction evidence and references
197 total interaction(s) for 150 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 3
  • Co-purification: 2
  • PCA: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 47
Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 15
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 34
  • Positive Genetic: 3
  • Synthetic Growth Defect: 39
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 10
  • Synthetic Rescue: 8
Resources
Expression Summary
histogram
Resources
Protein Information All STP22 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:106853 to 105696 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-12 | Sequence: 2004-02-18
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1158 106853..105696 2006-01-12 2004-02-18
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000514
References cited on this page View Complete Literature Guide for STP22
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Babst M, et al.  (2000) Mammalian tumor susceptibility gene 101 (TSG101) and the yeast homologue, Vps23p, both function in late endosomal trafficking. Traffic 1(3):248-58
4) Katzmann DJ, et al.  (2001) Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I. Cell 106(2):145-55
5) Li Y, et al.  (1999) Yeast mutants affecting possible quality control of plasma membrane proteins. Mol Cell Biol 19(5):3588-99