PHO89/YBR296C Summary Help

Standard Name PHO89
Systematic Name YBR296C
Alias ITN1 1
Feature Type ORF, Verified
Description Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification (2, 3, 4, 5)
Name Description PHOsphate metabolism
Chromosomal Location
ChrII:798522 to 796798 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PHO89 GO evidence and references
  View Computational GO annotations for PHO89
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 38 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
30 total interaction(s) for 24 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 1
  • Co-purification: 1
  • PCA: 2

Genetic Interactions
  • Negative Genetic: 8
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 574
Molecular Weight (Da) 62,653
Isoelectric Point (pI) 6.41
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:798522 to 796798 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1725 798522..796798 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000000500
References cited on this page View Complete Literature Guide for PHO89
1) Entian KD, et al.  (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702
2) Martinez P and Persson BL  (1998) Identification, cloning and characterization of a derepressible Na+-coupled phosphate transporter in Saccharomyces cerevisiae. Mol Gen Genet 258(6):628-38
3) Pattison-Granberg J and Persson BL  (2000) Regulation of cation-coupled high-affinity phosphate uptake in the yeast Saccharomyces cerevisiae. J Bacteriol 182(17):5017-9
4) Auesukaree C, et al.  (2003) Transcriptional regulation of phosphate-responsive genes in low-affinity phosphate-transporter-defective mutants in Saccharomyces cerevisiae. Biochem Biophys Res Commun 306(4):843-50
5) Sengottaiyan P, et al.  (2013) Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae. FEBS J 280(3):965-75