Standard Name	DPB3
Systematic Name	YBR278W
Feature Type	ORF, Verified
Description	Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	DNA Polymerase B (II) subunit

Chromosomal Location	ChrII:760294 to 760899 | ORF Map | GBrowse

	View Computational GO annotations for DPB3
Molecular Function
Manually curated	contributes_to DNA-directed DNA polymerase activity (IDA, IMP) contributes_to double-stranded DNA binding (IDA) contributes_to single-stranded DNA binding (IDA)
Biological Process
Manually curated	DNA-dependent DNA replication (IDA) error-prone translesion synthesis (IDA) heterochromatin organization involved in chromatin silencing (IGI, IMP) leading strand elongation (IGI, IMP) replicative senescence (IMP)
Cellular Component
Manually curated	epsilon DNA polymerase complex (IDA)

Classical genetics
null	chromosome/plasmid maintenance: abnormal mutation frequency: increased resistance to 4-aminofolic acid and sulfanilamide: decreased silencing: decreased viable
Large-scale survey
null	cell size: increased competitive fitness: decreased dessication resistance: decreased haploinsufficient resistance to cycloheximide: increased resistance to bleomycin: increased resistance to sirolimus: decreased resistance to wortmannin: decreased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to sodium selenide: decreased resistance to benzo[a]pyrene: decreased resistance to arsenite(3-): decreased stress resistance: decreased toxin resistance: decreased viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	288 total interaction(s) for 177 unique genes/features.
Physical Interactions	Affinity Capture-MS: 19 Affinity Capture-RNA: 3 Affinity Capture-Western: 7 Co-fractionation: 1 Co-purification: 4 Reconstituted Complex: 4 Two-hybrid: 5
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 167 Phenotypic Enhancement: 20 Phenotypic Suppression: 8 Positive Genetic: 30 Synthetic Growth Defect: 12 Synthetic Lethality: 5 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:760294 to 760899 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..606 760294..760899 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000482