CHK1/YBR274W Summary Help

Standard Name CHK1 1
Systematic Name YBR274W
Feature Type ORF, Verified
Description Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase (1, 2, 3, 4, 5)
Name Description CHeckpoint Kinase 1, 6
Chromosomal Location
ChrII:749594 to 751177 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CHK1 GO evidence and references
  View Computational GO annotations for CHK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
338 total interaction(s) for 168 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-Western: 2
  • Biochemical Activity: 3
  • Reconstituted Complex: 17
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 194
  • Phenotypic Enhancement: 25
  • Phenotypic Suppression: 6
  • Positive Genetic: 17
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 8
  • Synthetic Rescue: 14

Resources
Expression Summary
histogram
Resources
Length (a.a.) 527
Molecular Weight (Da) 60,016
Isoelectric Point (pI) 6.21
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:749594 to 751177 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1584 749594..751177 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000478
References cited on this page View Complete Literature Guide for CHK1
1) Liu Y, et al.  (2000) Characterization of a Saccharomyces cerevisiae homologue of Schizosaccharomyces pombe Chk1 involved in DNA-damage-induced M-phase arrest. Mol Gen Genet 262(6):1132-46
2) Wang H, et al.  (2001) Pds1 phosphorylation in response to DNA damage is essential for its DNA damage checkpoint function. Genes Dev 15(11):1361-72
3) Rouse J and Jackson SP  (2002) Interfaces between the detection, signaling, and repair of DNA damage. Science 297(5581):547-51
4) Agarwal R, et al.  (2003) Two distinct pathways for inhibiting pds1 ubiquitination in response to DNA damage. J Biol Chem 278(45):45027-33
5) Chen Y and Sanchez Y  (2004) Chk1 in the DNA damage response: conserved roles from yeasts to mammals. DNA Repair (Amst) 3(8-9):1025-32
6) Sanchez Y, et al.  (1999) Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms. Science 286(5442):1166-71