YPT10/YBR264C Summary Help

Standard Name YPT10 1, 2
Systematic Name YBR264C
Feature Type ORF, Verified
Description Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles (1, 2 and see Summary Paragraph)
Name Description Yeast Protein Two 1, 2
Chromosomal Location
ChrII:738369 to 737770 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All YPT10 GO evidence and references
  View Computational GO annotations for YPT10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Mutant phenotypes All YPT10 Phenotype evidence and references
Large-scale survey
null
overexpression
Resources
interactions All YPT10 Interaction evidence and references
47 total interaction(s) for 33 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 8
Genetic Interactions
  • Negative Genetic: 20
  • Positive Genetic: 2
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
Resources
Expression Summary
histogram
Resources
Protein Information All YPT10 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:738369 to 737770 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..600 738369..737770 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000468
SUMMARY PARAGRAPH for YPT10

Rab proteins are small Ras-related GTPases that function in multiple stages in membrane traffic. Rabs continuously cycle between the cytosol and membranes. The GDP-bound form of the Rab is complexed with in the cytosol and are recruited to the membranes with the help of GDF (GDI Displacement Factor) and by their prenylation by geranylgeranyl transferase II (GGTase II). The membrane-bound Rab is activated to the GTP-bound form by the guanine nucleotide exchange factors (GEFs) which then goes on to interact with downstream effectors. The GTPase-activating protein (GAP) stimulates the hydrolysis of GTP to GDP to inactivate the Rab. Eleven Rabs have been identified in yeast and more than 60 in mammalian cells (reviewed in 3, 4, 5).

Last updated: 2010-05-18

References cited on this page View Complete Literature Guide for YPT10
1) Louvet O, et al.  (1999) Characterization of the ORF YBR264c in Saccharomyces cerevisiae, which encodes a new yeast Ypt that is degraded by a proteasome-dependent mechanism. Mol Gen Genet 261(4-5):589-600
2) Lazar T, et al.  (1997) Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem Sci 22(12):468-72
3) Cai H, et al.  (2007) Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12(5):671-82
4) Grosshans BL, et al.  (2006) Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci U S A 103(32):11821-7
5) Bialek-Wyrzykowska U, et al.  (2000) Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance. Mol Microbiol 35(6):1295-311