DUT1/YBR252W Summary Help

Standard Name DUT1 1
Systematic Name YBR252W
Feature Type ORF, Verified
Description deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate (2, 3, 4)
Name Description DUTp pyrophosphatase 1
Chromosomal Location
ChrII:722611 to 723054 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DUT1 GO evidence and references
  View Computational GO annotations for DUT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 2 genes
Resources
Pathways
Classical genetics
null
reduction of function
Large-scale survey
null
reduction of function
repressible
Resources
50 total interaction(s) for 47 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 5
  • Phenotypic Enhancement: 1
  • Positive Genetic: 20
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 147
Molecular Weight (Da) 15,307
Isoelectric Point (pI) 7.53
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:722611 to 723054 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..444 722611..723054 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000456
References cited on this page View Complete Literature Guide for DUT1
1) McIntosh EM, et al.  (1986) Transcription of genes encoding enzymes involved in DNA synthesis during the cell cycle of Saccharomyces cerevisiae. Mol Gen Genet 204(3):363-6
2) Gadsden MH, et al.  (1993) dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae. EMBO J 12(11):4425-31
3) Guillet M, et al.  (2006) dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiae. Nucleic Acids Res 34(7):2056-66
4) Tchigvintsev A, et al.  (2011) Structure and activity of the Saccharomyces cerevisiae dUTP pyrophosphatase DUT1, an essential housekeeping enzyme. Biochem J 437(2):243-53