PRP5/YBR237W Summary Help

Standard Name PRP5 1
Systematic Name YBR237W
Alias RNA5 2
Feature Type ORF, Verified
Description RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA (3, 4, 5)
Name Description Pre-mRNA Processing 1
Chromosomal Location
ChrII:691969 to 694518 | ORF Map | GBrowse
Gbrowse
Genetic position: 138 cM
Gene Ontology Annotations All PRP5 GO evidence and references
  View Computational GO annotations for PRP5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 78 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
21 total interaction(s) for 17 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 1
  • PCA: 1
  • Protein-RNA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 849
Molecular Weight (Da) 96,358
Isoelectric Point (pI) 8.16
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:691969 to 694518 | ORF Map | GBrowse
SGD ORF map
Genetic position: 138 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2550 691969..694518 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000441
References cited on this page View Complete Literature Guide for PRP5
1) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
2) Hartwell LH, et al.  (1970) Identification of ten genes that control ribosome formation in yeast. Mol Gen Genet 109(1):42-56
3) de la Cruz J, et al.  (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24(5):192-8
4) Abu Dayyeh BK, et al.  (2002) Probing interactions between the U2 small nuclear ribonucleoprotein and the DEAD-box protein, Prp5. J Biol Chem 277(23):20221-33
5) Xu YZ, et al.  (2004) Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA. EMBO J 23(2):376-85