PDB1/YBR221C Summary

Standard Name	PDB1 1
Systematic Name	YBR221C
Feature Type	ORF, Verified
Description	E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria (2 and see Summary Paragraph)
Name Description	Pyruvate Dehydrogenase Beta subunit 2

Chromosomal Location	ChrII:666253 to 665153 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All PDB1 GO evidence and references

	View Computational GO annotations for PDB1
Molecular Function
Manually curated	pyruvate dehydrogenase (acetyl-transferring) activity (IMP)
Biological Process
Manually curated	acetyl-CoA biosynthetic process from pyruvate (IDA)
Cellular Component
Manually curated	mitochondrial nucleoid (IDA) mitochondrial pyruvate dehydrogenase complex (IDA) mitochondrion (IMP)
High-throughput	mitochondrion (IDA)

Pathways	pyruvate dehydrogenase complex

Mutant phenotypes All PDB1 Phenotype evidence and references

Large-scale survey
null	auxotrophy glycogen accumulation: decreased competitive fitness: decreased dessication resistance: decreased haploproficient heat sensitivity: increased nutrient utilization: decreased resistance to streptomycin: decreased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to hydroxyurea: decreased resistance to acetic acid: decreased resistance to quinine: decreased resistance to wortmannin: normal resistance to methyl methanesulfonate: decreased resistance to sirolimus: decreased respiratory growth: decreased rate stress resistance: increased toxin resistance: increased viable
overexpression	cell cycle progression: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PDB1 Interaction evidence and references

	197 total interaction(s) for 155 unique genes/features.
Physical Interactions	Affinity Capture-MS: 51 Affinity Capture-RNA: 3 Two-hybrid: 2
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 101 Positive Genetic: 31 Synthetic Growth Defect: 6 Synthetic Lethality: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PDB1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:666253 to 665153 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1101	666253..665153	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000425

SUMMARY PARAGRAPH for PDB1

PDB1 encodes the beta subunit of pyruvate dehydrogenase (3, 4). Pyruvate dehydrogenase, consisting of alpha (Pda1p) and beta (Pdb1p) subunits, is the E1 component of the large multienzyme pyruvate dehydrogenase complex (5). This mitochondrial complex consists of an E2 (Lat1p) core that binds E1 (Pda1p and Pdb1p) , E3 (Lpd1p), and Protein X (Pdx1p) components (5). E1 (pyruvate dehydrogenase), E2 (dihydrolipoamide acetyltransferase), and E3 (dihydrolipoamide dehydrogenase) act in concert to convert pyruvate into acetyl-CoA (5). Cells lacking PDB1 are viable but lack pyruvate dehydrogenase activity (2). The yeast PDB1 gene shows strong similarity to human PDHB, which encodes the human E1 beta sub unit (2).

Last updated: 2005-07-01

References cited on this page View Complete Literature Guide for PDB1

1)	Steensma, H.Y. (1992) Personal Communication, Mortimer Map Edition 11
2)	Miran SG, et al. (1993) Characterization of PDH beta 1, the structural gene for the pyruvate dehydrogenase beta subunit from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 90(4):1252-6
3)	Behal RH, et al. (1989) Nucleotide and deduced amino acid sequence of the alpha subunit of yeast pyruvate dehydrogenase. Biochem Biophys Res Commun 164(2):941-6
4)	Steensma HY, et al. (1990) Molecular cloning of the gene for the E1 alpha subunit of the pyruvate dehydrogenase complex from Saccharomyces cerevisiae. Eur J Biochem 191(3):769-74
5)	Pronk JT, et al. (1996) Pyruvate metabolism in Saccharomyces cerevisiae. Yeast 12(16):1607-33