AME1/YBR211C Summary

AME1 BASIC INFORMATION

Standard Name	AME1 1
Systematic Name	YBR211C
Alias	ARP100
Feature Type	ORF, Verified
Description	Essential kinetochore protein associated with microtubules and spindle pole bodies; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance (1, 2)
Name Description	Associated with Microtubules and Essential 1

GO Annotations	All AME1 GO evidence and references
	View Computational GO annotations for AME1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	attachment of spindle microtubules to kinetochore (IDA, IPI) protein localization to kinetochore (IMP)
Cellular Component
Manually curated	COMA complex (IDA) kinetochore (IDA) spindle pole body (NAS)

Mutant Phenotype	All AME1 Phenotype details and references
Classical genetics
conditional	cell cycle progression in anaphase: absent heat sensitivity: increased
null	inviable
Large-scale survey
null	inviable
overexpression	cell cycle progression: abnormal
repressible	cell cycle progression in G2 phase: abnormal

Interactions	AME1 All interactions details and references
	50 total interaction(s) for 23 unique genes/features.
Physical Interactions	Affinity Capture-MS: 22 Affinity Capture-RNA: 1 Affinity Capture-Western: 12 Biochemical Activity: 2 Co-fractionation: 1 Two-hybrid: 9
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Synthetic Lethality: 1

Sequence Information

ChrII:647127 to 646153 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-07-16 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..975	647127..646153	2004-07-16	1997-01-28

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000415

AME1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for AME1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for AME1]

1)	Pot I, et al. (2005) Spindle checkpoint maintenance requires Ame1 and Okp1. Cell Cycle 4(10):1448-56
2)	De Wulf P, et al. (2003) Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev 17(23):2902-21

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