MSI1/YBR195C Summary Help

Standard Name MSI1 1
Systematic Name YBR195C
Alias CAC3
Feature Type ORF, Verified
Description Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and cytoplasm and independently regulates the RAS/cAMP pathway via sequestration of Npr1p kinase (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
Name Description Multicopy Suppressor of IRA1 13
Chromosomal Location
ChrII:611882 to 610614 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 89 cM
Gene Ontology Annotations All MSI1 GO evidence and references
  View Computational GO annotations for MSI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
162 total interaction(s) for 102 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 8
  • Reconstituted Complex: 2
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Rescue: 12
  • Negative Genetic: 54
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 14
  • Synthetic Growth Defect: 34
  • Synthetic Lethality: 3
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 422
Molecular Weight (Da) 47,364
Isoelectric Point (pI) 5.42
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:611882 to 610614 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 89 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1269 611882..610614 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000399
References cited on this page View Complete Literature Guide for MSI1
1) Spevak, W.  (1992) Personal Communication, Mortimer Map Edition 11
2) Enomoto S and Berman J  (1998) Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Genes Dev 12(2):219-32
3) Kaufman PD, et al.  (1997) Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes Dev 11(3):345-57
4) Game JC and Kaufman PD  (1999) Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics 151(2):485-97
5) Smith JS, et al.  (1999) A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19(4):3184-97
6) Johnston SD, et al.  (2001) CAC3(MSI1) suppression of RAS2(G19V) is independent of chromatin assembly factor I and mediated by NPR1. Mol Cell Biol 21(5):1784-94
7) Sharp JA, et al.  (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
8) Zabaronick SR and Tyler JK  (2005) The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol 25(2):652-60
9) Zhou H, et al.  (2006) Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. Biochemistry 45(9):2852-61
10) Kim JA and Haber JE  (2009) Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete. Proc Natl Acad Sci U S A 106(4):1151-6
11) Kim HJ, et al.  (2009) Potential role of the histone chaperone, CAF-1, in transcription. BMB Rep 42(4):227-31
12) Marquardt S, et al.  (2014) A chromatin-based mechanism for limiting divergent noncoding transcription. Cell 157(7):1712-23
13) Ruggieri R, et al.  (1989) MSI1, a negative regulator of the RAS-cAMP pathway in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 86(22):8778-82