MSI1/YBR195C Summary

MSI1/YBR195C Summary

Standard Name	MSI1 1
Systematic Name	YBR195C
Alias	CAC3
Feature Type	ORF, Verified
Description	Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
Name Description	Multicopy Suppressor of IRA1 12

Chromosomal Location	ChrII:611882 to 610614 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: 89 cM

Gene Ontology Annotations All MSI1 GO evidence and references

	View Computational GO annotations for MSI1
Molecular Function
Manually curated	contributes_to histone binding (IDA)
Biological Process
Manually curated	DNA replication-dependent nucleosome assembly (IDA)
Cellular Component
Manually curated	CAF-1 complex (IDA) cytoplasm (IDA) nucleus (IDA)

Mutant phenotypes All MSI1 Phenotype evidence and references

Classical genetics
null	UV resistance: decreased chromosome/plasmid maintenance: abnormal silencing: abnormal silencing: decreased viable
Large-scale survey
null	UV resistance: decreased cell cycle progression through the G2/M phase transition: delayed competitive fitness: decreased resistance to methyl methanesulfonate: increased resistance to mycophenolic acid: increased resistance to hydroxyurea: increased resistance to bleomycin: increased resistance to fluconazole: increased resistance to fenpropimorph: increased resistance to caffeine: increased resistance to cycloheximide: increased resistance to camptothecin: increased sporulation: normal viable
overexpression	vegetative growth: decreased rate vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MSI1 Interaction evidence and references

	161 total interaction(s) for 101 unique genes/features.
Physical Interactions	Affinity Capture-MS: 14 Affinity Capture-RNA: 1 Affinity Capture-Western: 8 Reconstituted Complex: 2 Two-hybrid: 8
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 12 Negative Genetic: 54 Phenotypic Enhancement: 4 Phenotypic Suppression: 2 Positive Genetic: 14 Synthetic Growth Defect: 34 Synthetic Lethality: 3 Synthetic Rescue: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MSI1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:611882 to 610614 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

: 89 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1269	611882..610614	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000399

References cited on this page View Complete Literature Guide for MSI1

1)	Spevak, W. (1992) Personal Communication, Mortimer Map Edition 11
2)	Enomoto S and Berman J (1998) Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Genes Dev 12(2):219-32
3)	Kaufman PD, et al. (1997) Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes Dev 11(3):345-57
4)	Game JC and Kaufman PD (1999) Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics 151(2):485-97
5)	Smith JS, et al. (1999) A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19(4):3184-97
6)	Johnston SD, et al. (2001) CAC3(MSI1) suppression of RAS2(G19V) is independent of chromatin assembly factor I and mediated by NPR1. Mol Cell Biol 21(5):1784-94
7)	Sharp JA, et al. (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
8)	Zabaronick SR and Tyler JK (2005) The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol 25(2):652-60
9)	Zhou H, et al. (2006) Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. Biochemistry 45(9):2852-61
10)	Kim JA and Haber JE (2009) Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete. Proc Natl Acad Sci U S A 106(4):1151-6
11)	Kim HJ, et al. (2009) Potential role of the histone chaperone, CAF-1, in transcription. BMB Rep 42(4):227-31
12)	Ruggieri R, et al. (1989) MSI1, a negative regulator of the RAS-cAMP pathway in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 86(22):8778-82