PCH2/YBR186W Summary Help

Standard Name PCH2 1
Systematic Name YBR186W
Feature Type ORF, Verified
Description Hexameric ring ATPase that remodels chromosome axis protein Hop1p; nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in rDNA; required for meiotic double-stranded break formation (1, 2, 3, 4 and see Summary Paragraph)
Name Description Pachytene CHeckpoint 1
Chromosomal Location
ChrII:600553 to 602360 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PCH2 GO evidence and references
  View Computational GO annotations for PCH2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
53 total interaction(s) for 30 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 1
  • PCA: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 5
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 14
  • Positive Genetic: 1
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 1
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 564
Molecular Weight (Da) 63,460
Isoelectric Point (pI) 5.76
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:600553 to 602360 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2000-07-14
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1551 600553..602103 2011-02-03 2000-07-14
Intron 1552..1664 602104..602216 2011-02-03 2000-07-14
CDS 1665..1808 602217..602360 2011-02-03 2000-07-14
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000390
SUMMARY PARAGRAPH for PCH2

PCH2 is an essential nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective (2). Nucleolar localization of Pch2p depends on silencing factors Sir2p and Dot1p, mutation of each of which disrupts the pachytene checkpoint (2, 1). Mutation of PCH2 relieves the checkpoint-induced pachytene arrest of zip1, zip2, and dmc1 mutants, resulting in chromosome missegregation and low spore viability (1). Pch2p also functions to repress interhomolog recombination in the ribosomal DNA during meiosis by excluding the meiosis-specific DNA binding protein Hop1p, which is required for homologous chromosome synapsis and the formation of chiasmata (1). In some situations, Pch2p will also localize to telomeres in a Sir3p-dependent manner and provide checkpoint function, thereby linking the nucleolus, chromatin silencing, and the pachytene checkpoint (1).

Last updated: 2004-07-01 Contact SGD

References cited on this page View Complete Literature Guide for PCH2
1) San-Segundo PA and Roeder GS  (1999) Pch2 links chromatin silencing to meiotic checkpoint control. Cell 97(3):313-24
2) San-Segundo PA and Roeder GS  (2000) Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell 11(10):3601-15
3) Farmer S, et al.  (2012) Budding yeast pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination. PLoS One 7(6):e39724
4) Chen C, et al.  (2014) Pch2 is a hexameric ring ATPase that remodels the chromosome axis protein Hop1. Proc Natl Acad Sci U S A 111(1):E44-53