SEC66/YBR171W Summary

SEC66 BASIC INFORMATION

Standard Name	SEC66
Systematic Name	YBR171W
Alias	HSS1 , SEC71
Feature Type	ORF, Verified
Description	Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER (1)
Name Description	SECretory

GO Annotations	All SEC66 GO evidence and references
	View Computational GO annotations for SEC66
Molecular Function
Manually curated	protein transporter activity (TAS)
Biological Process
Manually curated	filamentous growth (IMP) posttranslational protein targeting to membrane, translocation (IDA)
Cellular Component
Manually curated	endoplasmic reticulum membrane (TAS) Sec62/Sec63 complex (IPI)

Mutant Phenotype	All SEC66 Phenotype details and references
Classical genetics
null	heat sensitivity: increased Kar2p precursor accumulation: increased invertase precursor accumulation: increased resistance to Calcofluor White: decreased
Large-scale survey
null	glycogen accumulation: increased polyphosphate accumulation: abnormal competitive fitness: decreased freeze-thaw resistance: decreased heat sensitivity: increased resistance to caffeine: decreased resistance to ethanol: decreased resistance to hydroxyurea: decreased resistance to hygromycin B: decreased resistance to nickel(2+): decreased resistance to methyl methanesulfonate: decreased resistance to methanol: decreased resistance to propan-1-ol: decreased resistance to nickel sulfate: increased respiratory growth: decreased rate viable

Interactions	SEC66 All interactions details and references
	250 total interaction(s) for 153 unique genes/features.
Physical Interactions	Affinity Capture-MS: 13 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Co-fractionation: 1 Co-purification: 5 Two-hybrid: 9
Genetic Interactions	Dosage Rescue: 1 Phenotypic Enhancement: 163 Phenotypic Suppression: 30 Synthetic Growth Defect: 9 Synthetic Lethality: 16

Sequence Information

ChrII:578359 to 578979 | |

Last Update

Coordinates: 2004-07-16 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..621	578359..578979	2004-07-16	1997-01-28

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000375

SEC66 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SEC66

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SEC66]

1)	Young BP, et al. (2001) Sec63p and Kar2p are required for the translocation of SRP-dependent precursors into the yeast endoplasmic reticulum in vivo. EMBO J 20(1-2):262-71

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