SUP45/YBR143C Summary Help

Standard Name SUP45
Systematic Name YBR143C
Alias SAL4 , SUP1 , SUP47
Feature Type ORF, Verified
Description Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p (1, 2, 3, 4, 5, 6)
Name Description SUPpressor
Gene Product Alias eRF1
Chromosomal Location
ChrII:532182 to 530869 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 81 cM
Gene Ontology Annotations All SUP45 GO evidence and references
  View Computational GO annotations for SUP45
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 91 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
108 total interaction(s) for 59 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 56
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 11
  • Biochemical Activity: 2
  • Reconstituted Complex: 6
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Rescue: 10
  • Negative Genetic: 3
  • Phenotypic Enhancement: 3
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 437
Molecular Weight (Da) 49,005
Isoelectric Point (pI) 4.77
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:532182 to 530869 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 81 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1314 532182..530869 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000347
References cited on this page View Complete Literature Guide for SUP45
1) Stansfield I, et al.  (1992) Ribosomal association of the yeast SAL4 (SUP45) gene product: implications for its role in translation fidelity and termination. Mol Microbiol 6(23):3469-78
2) Himmelfarb HJ, et al.  (1985) Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product. Mol Cell Biol 5(4):816-22
3) Stansfield I, et al.  (1995) The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae. EMBO J 14(17):4365-73
4) Valouev IA, et al.  (2004) Translation termination factors function outside of translation: yeast eRF1 interacts with myosin light chain, Mlc1p, to effect cytokinesis. Mol Microbiol 53(2):687-96
5) Heurgue-Hamard V, et al.  (2006) The Zinc Finger Protein Ynr046w Is Plurifunctional and a Component of the eRF1 Methyltransferase in Yeast. J Biol Chem 281(47):36140-8
6) Studte P, et al.  (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol 69(5):1266-77