Standard Name	SUP45
Systematic Name	YBR143C
Alias	SAL4 , SUP1 , SUP47
Feature Type	ORF, Verified
Description	Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p (1, 2, 3, 4, 5, 6)
Name Description	SUPpressor
Gene Product Alias	eRF1

Chromosomal Location	ChrII:532182 to 530869 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 81 cM

	View Computational GO annotations for SUP45
Molecular Function
Manually curated	translation release factor activity (IDA) translation release factor activity, codon specific (IMP, ISS)
Biological Process
Manually curated	cytokinesis (IGI, IMP, IPI) translational termination (IDA, IGI, IMP, ISS)
Cellular Component
Manually curated	cytosol (IPI) translation release factor complex (IDA)

Classical genetics
conditional	cytokinesis: absent heat sensitivity: increased
reduction of function	tRNA-Lys and tRNA-Glu accumulation: increased cell shape: abnormal cold sensitivity: increased hyperosmotic stress resistance: decreased resistance to hydroxyurea: decreased resistance to paromomycin: decreased resistance to caffeine: decreased respiratory growth: absent toxin resistance: increased
Large-scale survey
conditional	colony sectoring: increased
null	filamentous growth: increased haploinsufficient inviable starvation resistance: decreased
overexpression	cell cycle progression: abnormal vegetative growth: decreased rate
reduction of function	competitive fitness: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	104 total interaction(s) for 57 unique genes/features.
Physical Interactions	Affinity Capture-MS: 53 Affinity Capture-RNA: 1 Affinity Capture-Western: 11 Biochemical Activity: 2 Reconstituted Complex: 6 Two-hybrid: 6
Genetic Interactions	Dosage Rescue: 10 Negative Genetic: 3 Phenotypic Enhancement: 3 Synthetic Growth Defect: 3 Synthetic Lethality: 1 Synthetic Rescue: 5
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:532182 to 530869 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 81 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1314 532182..530869 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000347