Standard Name	CCZ1 1
Systematic Name	YBR131W
Alias	CVT16
Feature Type	ORF, Verified
Description	Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway (2, 3)
Name Description	Calcium Caffeine Zinc sensitivity 1

Chromosomal Location	ChrII:497163 to 499277 | ORF Map | GBrowse

	View Computational GO annotations for CCZ1
Molecular Function
Manually curated	guanyl-nucleotide exchange factor activity (IPI)
Biological Process
Manually curated	autophagic vacuole fusion (IDA) autophagy (IDA) protein targeting to vacuole (IMP) vacuolar transport (IGI, IMP) vesicle docking (IMP)
Cellular Component
Manually curated	Mon1-Ccz1 complex (IPI)

Classical genetics
null	autophagy: absent metal resistance: decreased Fus-Mid-GFP distribution: abnormal carboxypeptidase Y (Prc1p) modification: decreased resistance to calcium(2+): decreased resistance to calcium dichloride: decreased resistance to zinc dichloride: decreased resistance to rubidium chloride: decreased resistance to caffeine: decreased resistance to verapamil: decreased sporulation: absent vacuolar morphology: abnormal viable
overexpression	bud neck morphology: abnormal budding: abnormal cellular morphology: abnormal
Large-scale survey
null	polyphosphate accumulation: abnormal glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased mitophagy: absent Fus-Mid-GFP distribution: abnormal Pho8p (ALP) modification: decreased Cps1p (CPS) modification: decreased resistance to bleomycin: decreased resistance to sirolimus: decreased resistance to caffeine: decreased resistance to monensin: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to sirolimus: increased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to hydrogen chloride: increased resistance to acetic acid: increased resistance to wortmannin: increased resistance to cycloheximide: decreased resistance to streptomycin: decreased resistance to dimethyl sulfoxide: decreased resistance to mefloquine: decreased resistance to calcium dichloride: decreased resistance to clioquinol: decreased sporulation: absent sporulation efficiency: decreased stress resistance: decreased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: decreased vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	208 total interaction(s) for 129 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-Western: 5 Co-fractionation: 1 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 4 Negative Genetic: 135 Phenotypic Enhancement: 3 Phenotypic Suppression: 1 Positive Genetic: 27 Synthetic Growth Defect: 16 Synthetic Lethality: 6 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:497163 to 499277 | ORF Map | GBrowse
	This feature contains embedded feature(s): YBR131C-A
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2115 497163..499277 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000335