MMS4/YBR098W Summary

MMS4/YBR098W Summary

Standard Name	MMS4 1
Systematic Name	YBR098W
Alias	SLX2 , YBR100W
Feature Type	ORF, Verified
Description	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p (1, 2, 3, 4, 5, 6)
Name Description	Methyl MethaneSulfonate sensitivity 1

Chromosomal Location	ChrII:441515 to 443590 \| ORF Map \| GBrowse

Gene Ontology Annotations All MMS4 GO evidence and references

	View Computational GO annotations for MMS4
Molecular Function
Manually curated	crossover junction endodeoxyribonuclease activity (IDA) endonuclease activity (IDA) transcription coactivator activity (IDA)
Biological Process
Manually curated	DNA repair (IMP) DNA topological change (IGI) reciprocal meiotic recombination (IGI, IMP) regulation of reciprocal meiotic recombination (IGI) resolution of meiotic recombination intermediates (IGI) response to DNA damage stimulus (IMP)
Cellular Component
Manually curated	Holliday junction resolvase complex (IDA) nucleus (ISS)

Mutant phenotypes All MMS4 Phenotype evidence and references

Classical genetics
null	chromosome/plasmid maintenance: abnormal colony sectoring: increased gamma ray resistance: decreased resistance to hydroxyurea: decreased resistance to etoposide: decreased resistance to methyl methanesulfonate: decreased resistance to selenium(2+): decreased sporulation efficiency: decreased viability: decreased
reduction of function	UV resistance: normal X ray resistance: normal resistance to methyl methanesulfonate: decreased
Large-scale survey
null	UV resistance: decreased competitive fitness: decreased dessication resistance: decreased gamma ray resistance: decreased resistance to doxorubicin: decreased resistance to camptothecin: decreased resistance to tirapazamine: decreased resistance to CTBT: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to fenpropimorph: increased resistance to quinine: decreased resistance to cycloheximide: decreased resistance to sodium selenide: decreased resistance to cisplatin: decreased sporulation: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MMS4 Interaction evidence and references

	232 total interaction(s) for 123 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 1 Affinity Capture-Western: 4 Biochemical Activity: 2 Co-crystal Structure: 1 Co-purification: 2 Reconstituted Complex: 2 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 110 Phenotypic Enhancement: 10 Phenotypic Suppression: 6 Positive Genetic: 2 Synthetic Growth Defect: 44 Synthetic Lethality: 36 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MMS4 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:441515 to 443590 | |

This feature contains embedded feature(s): YBR100W | YBR099C

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 2003-01-03

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2076	441515..443590	2011-02-03	2003-01-03

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000302

References cited on this page View Complete Literature Guide for MMS4

1)	Xiao W, et al. (1998) Mms4, a putative transcriptional (co)activator, protects Saccharomyces cerevisiae cells from endogenous and environmental DNA damage. Mol Gen Genet 257(6):614-23
2)	Kaliraman V, et al. (2001) Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease. Genes Dev 15(20):2730-40
3)	Bastin-Shanower SA, et al. (2003) The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10. Mol Cell Biol 23(10):3487-96
4)	Oh SD, et al. (2008) RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell 31(3):324-36
5)	Jessop L and Lichten M (2008) Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell 31(3):313-23
6)	Gallo-Fernandez M, et al. (2012) Cell cycle-dependent regulation of the nuclease activity of Mus81-Eme1/Mms4. Nucleic Acids Res 40(17):8325-35