MMS4/YBR098W Summary Help

Standard Name MMS4 1
Systematic Name YBR098W
Alias SLX2 , YBR100W
Feature Type ORF, Verified
Description Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates the function of Mms4p-Mus81p (1, 2, 3, 4, 5, 6)
Name Description Methyl MethaneSulfonate sensitivity 1
Chromosomal Location
ChrII:441515 to 443590 | ORF Map | GBrowse
Gene Ontology Annotations All MMS4 GO evidence and references
  View Computational GO annotations for MMS4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
236 total interaction(s) for 123 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-purification: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 110
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 6
  • Positive Genetic: 3
  • Synthetic Growth Defect: 44
  • Synthetic Lethality: 36
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 691
Molecular Weight (Da) 78,763
Isoelectric Point (pI) 7.53
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:441515 to 443590 | ORF Map | GBrowse
This feature contains embedded feature(s): YBR100W | YBR099C
Last Update Coordinates: 2011-02-03 | Sequence: 2003-01-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2076 441515..443590 2011-02-03 2003-01-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000302
References cited on this page View Complete Literature Guide for MMS4
1) Xiao W, et al.  (1998) Mms4, a putative transcriptional (co)activator, protects Saccharomyces cerevisiae cells from endogenous and environmental DNA damage. Mol Gen Genet 257(6):614-23
2) Kaliraman V, et al.  (2001) Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease. Genes Dev 15(20):2730-40
3) Bastin-Shanower SA, et al.  (2003) The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10. Mol Cell Biol 23(10):3487-96
4) Oh SD, et al.  (2008) RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell 31(3):324-36
5) Jessop L and Lichten M  (2008) Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell 31(3):313-23
6) Gallo-Fernandez M, et al.  (2012) Cell cycle-dependent regulation of the nuclease activity of Mus81-Eme1/Mms4. Nucleic Acids Res 40(17):8325-35