VPS15/YBR097W Summary Help

VPS15 BASIC INFORMATION

Standard Name VPS15 1, 2
Systematic Name YBR097W
Alias GRD8 , VAC4 , VPL19 2 , VPS40 3
Feature Type ORF, Verified
Description Myristoylated serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p (4, 5, 6, 7, 8)
Name Description Vacuolar Protein Sorting 1, 2
GO Annotations All VPS15 GO evidence and references
    View Computational GO annotations for VPS15
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All VPS15 Phenotype details and references
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Interactions VPS15 All interactions details and references
39 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-Western: 8
  • Co-fractionation: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 5

Sequence Information
ChrII:436945 to 441309 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2004-07-16 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4365 436945..441309 2004-07-16 1997-01-28
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000301

VPS15 RESOURCES

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  • Functional Analysis

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Expression Summary histogram

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for VPS15]

1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
4) Stack JH and Emr SD  (1994) Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. J Biol Chem 269(50):31552-62
5) Stack JH, et al.  (1995) Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins. Annu Rev Cell Dev Biol 11():1-33
6) Stack JH, et al.  (1995) Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J Cell Biol 129(2):321-34
7) Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
8) Slessareva JE, et al.  (2006) Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome. Cell 126(1):191-203