RFC5/YBR087W Summary

RFC5 BASIC INFORMATION

Standard Name	RFC5 1
Systematic Name	YBR087W
Feature Type	ORF, Verified
Description	Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon (2, 3)
Name Description	Replication Factor C 2

GO Annotations	All RFC5 GO evidence and references
	View Computational GO annotations for RFC5
Molecular Function
Manually curated	contributes_to DNA clamp loader activity (IDA)
Biological Process
Manually curated	leading strand elongation (IDA) mismatch repair (TAS) sister chromatid cohesion (IMP, IPI)
Cellular Component
Manually curated	Ctf18 RFC-like complex (IPI) DNA replication factor C complex (IDA, IMP) Elg1 RFC-like complex (IPI) Rad17 RFC-like complex (IDA)

Mutant Phenotype	All RFC5 Phenotype details and references
Classical genetics
conditional	cell cycle progression in S phase: abnormal chromosome segregation: abnormal chromosome/plasmid maintenance: abnormal heat sensitivity: increased
Large-scale survey
null	inviable
reduction of function	competitive fitness: decreased resistance to tunicamycin: decreased
repressible	cell cycle progression in G2 phase: abnormal

Interactions	RFC5 All interactions details and references
	View additional details at BioGRID
	217 total interaction(s) for 137 unique genes/features.
Physical Interactions	Affinity Capture-MS: 37 Affinity Capture-Western: 15 Co-fractionation: 1 Co-purification: 6 Reconstituted Complex: 5 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 9 Negative Genetic: 92 Phenotypic Enhancement: 8 Phenotypic Suppression: 4 Positive Genetic: 9 Synthetic Growth Defect: 2 Synthetic Lethality: 25 Synthetic Rescue: 1

Sequence Information

ChrII:423759 to 424823 | |

Last Update

Coordinates: 2004-07-16 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1065	423759..424823	2004-07-16	1997-01-28

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000291

RFC5 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for RFC5

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RFC5]

1)	Gary SL and Burgers MJ (1995) Identification of the fifth subunit of Saccharomyces cerevisiae replication factor C. Nucleic Acids Res 23(24):4986-91
2)	Cullmann G, et al. (1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol 15(9):4661-71
3)	Yao N, et al. (2003) Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen. J Biol Chem 278(50):50744-53

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