RPG1/YBR079C Summary Help

Standard Name RPG1 1
Systematic Name YBR079C
Alias TIF32 2
Feature Type ORF, Verified
Description eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation (1, 3, 4, 5, 6)
Chromosomal Location
ChrII:398277 to 395383 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RPG1 GO evidence and references
  View Computational GO annotations for RPG1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
256 total interaction(s) for 119 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 133
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 51
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-fractionation: 8
  • Co-purification: 6
  • Reconstituted Complex: 10
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 4
  • Negative Genetic: 16
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 964
Molecular Weight (Da) 110,343
Isoelectric Point (pI) 6.24
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:398277 to 395383 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2895 398277..395383 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000283
References cited on this page View Complete Literature Guide for RPG1
1) Kovarik P, et al.  (1998) RPG1: an essential gene of saccharomyces cerevisiae encoding a 110-kDa protein required for passage through the G1 phase. Curr Genet 33(2):100-9
2) Vornlocher HP, et al.  (1999) A 110-kilodalton subunit of translation initiation factor eIF3 and an associated 135-kilodalton protein are encoded by the Saccharomyces cerevisiae TIF32 and TIF31 genes. J Biol Chem 274(24):16802-12
3) Phan L, et al.  (1998) Identification of a translation initiation factor 3 (eIF3) core complex, conserved in yeast and mammals, that interacts with eIF5. Mol Cell Biol 18(8):4935-46
4) Phan L, et al.  (2001) A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met. EMBO J 20(11):2954-65
5) Valasek L, et al.  (2003) The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo. Genes Dev 17(6):786-99
6) Cuchalova L, et al.  (2010) The RNA Recognition Motif of Eukaryotic Translation Initiation Factor 3g (eIF3g) Is Required for Resumption of Scanning of Posttermination Ribosomes for Reinitiation on GCN4 and Together with eIF3i Stimulates Linear Scanning. Mol Cell Biol 30(19):4671-86