ECM33/YBR078W Summary Help

Standard Name ECM33 1
Systematic Name YBR078W
Feature Type ORF, Verified
Description GPI-anchored protein of unknown function; possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p; ECM33 has a paralog, PST1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description ExtraCellular Mutant 1
Chromosomal Location
ChrII:393123 to 394742 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ECM33 GO evidence and references
  View Computational GO annotations for ECM33
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 16 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
323 total interaction(s) for 238 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 5
  • Co-fractionation: 2
  • PCA: 1

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 270
  • Phenotypic Enhancement: 2
  • Positive Genetic: 29
  • Synthetic Growth Defect: 1
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 429
Molecular Weight (Da) 43,768
Isoelectric Point (pI) 4.48
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:393123 to 394742 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..58 393123..393180 2011-02-03 1997-01-28
Intron 59..388 393181..393510 2011-02-03 1997-01-28
CDS 389..1620 393511..394742 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000282
References cited on this page View Complete Literature Guide for ECM33
1) Lussier M, et al.  (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2) Bidlingmaier S and Snyder M  (2002) Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics 1(6):345-56
3) Toh-E A and Oguchi T  (2002) Genetic characterization of genes encoding enzymes catalyzing addition of phospho-ethanolamine to the glycosylphosphatidylinositol anchor in Saccharomyces cerevisiae. Genes Genet Syst 77(5):309-22
4) Terashima H, et al.  (2003) The localization change of Ybr078w/Ecm33, a yeast GPI-associated protein, from the plasma membrane to the cell wall, affecting the cellular function. FEMS Microbiol Lett 218(1):175-80
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906