RDH54/YBR073W Summary Help

Standard Name RDH54 1
Systematic Name YBR073W
Alias TID1 2
Feature Type ORF, Verified
Description DNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA and promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis and meiosis; phosphorylated in Mec1p-, Rad53p-dependent way in response to one DSB; contributes to remodelling of nucleosomes; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p (1, 2, 3, 4, 5, 6, 7, 8)
Name Description RaD54 Homolog 1
Chromosomal Location
ChrII:383112 to 385988 | ORF Map | GBrowse
Gene Ontology Annotations All RDH54 GO evidence and references
  View Computational GO annotations for RDH54
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 13 genes
Classical genetics
reduction of function
Large-scale survey
107 total interaction(s) for 75 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Co-localization: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 51
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 8
  • Positive Genetic: 7
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 1
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 958
Molecular Weight (Da) 107,946
Isoelectric Point (pI) 9.68
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:383112 to 385988 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2877 383112..385988 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000277
References cited on this page View Complete Literature Guide for RDH54
1) Klein HL  (1997) RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis. Genetics 147(4):1533-43
2) Dresser ME, et al.  (1997) DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics 147(2):533-44
3) Petukhova G, et al.  (2000) Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1. Genes Dev 14(17):2206-15
4) Shinohara M, et al.  (2003) Crossover interference in Saccharomyces cerevisiae requires a TID1/RDH54- and DMC1-dependent pathway. Genetics 163(4):1273-86
5) Chi P, et al.  (2006) Yeast recombination factor Rdh54 functionally interacts with the Rad51 recombinase and catalyzes Rad51 removal from DNA. J Biol Chem 281(36):26268-79
6) Nimonkar AV, et al.  (2012) Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination. J Biol Chem 287(34):28727-37
7) Ferrari M, et al.  (2013) Tid1/Rdh54 translocase is phosphorylated through a Mec1- and Rad53-dependent manner in the presence of DSB lesions in budding yeast. DNA Repair (Amst) 12(5):347-55
8) Busygina V, et al.  (2013) Functional attributes of the Saccharomyces cerevisiae meiotic recombinase Dmc1. DNA Repair (Amst) 12(9):707-12