GIP1/YBR045C Summary

Standard Name	GIP1 1
Systematic Name	YBR045C
Feature Type	ORF, Verified
Description	Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p (1, 2 and see Summary Paragraph)
Name Description	Glc7-Interacting Protein 1

Chromosomal Location	ChrII:330092 to 328173 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All GIP1 GO evidence and references


Molecular Function
Manually curated	protein phosphatase 1 binding (IMP, IPI) protein phosphatase type 1 regulator activity (IDA)
Biological Process
Manually curated	ascospore wall assembly (IMP) regulation of phosphoprotein phosphatase activity (IMP)
Cellular Component
Manually curated	prospore membrane (IDA) protein phosphatase type 1 complex (IPI)

Mutant phenotypes All GIP1 Phenotype evidence and references

Classical genetics
conditional	spore wall formation: abnormal sporulation: absent
null	sporulation: absent
Large-scale survey
null	competitive fitness: increased resistance to paromomycin: decreased resistance to cycloheximide: decreased resistance to amiodarone: decreased sporulation: absent sporulation: abnormal sporulation efficiency: decreased viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GIP1 Interaction evidence and references

	32 total interaction(s) for 22 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Two-hybrid: 6
Genetic Interactions	Negative Genetic: 17 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 1 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GIP1 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:330092 to 328173 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 2011-02-03

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1920	330092..328173	2011-02-03	2011-02-03

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000249

SUMMARY PARAGRAPH for GIP1

Gip1p is a regulatory protein for the Glc7p type-1 protein phosphatase (PP1), and is required for normal localization of Glc7p during meiosis (2). The Gip1p-Glc7p phosphatase complex regulates septin organization and spore wall formation (2). GIP1 is also required for the expression of some late meiotic genes, including DIT1 and SPS100 (3). GIP1 is transcribed in an Ime1p-dependent manner during meiosis (3, 1), at which time Gip1p and Glc7p colocalize with the septins in structures underlying the growing prospore membranes (2).

gip1 haploid null mutants are viable, but homozygous diploid null mutants are unable to sporulate (1). Meiotic progression is normal in gip1 nulls, but Glc7p and septins display abnormal localization, and spore wall assembly is compromised (2).

Last updated: 2005-10-10

References cited on this page View Complete Literature Guide for GIP1

1)	Tu J, et al. (1996) Protein phosphatase type 1 interacts with proteins required for meiosis and other cellular processes in Saccharomyces cerevisiae. Mol Cell Biol 16(8):4199-206
2)	Tachikawa H, et al. (2001) A Gip1p-Glc7p phosphatase complex regulates septin organization and spore wall formation. J Cell Biol 155(5):797-808
3)	Ramaswamy NT, et al. (1998) Regulation of yeast glycogen metabolism and sporulation by Glc7p protein phosphatase. Genetics 149(1):57-72