FAT1/YBR041W Summary Help

Standard Name FAT1 1
Systematic Name YBR041W
Feature Type ORF, Verified
Description Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description FATty acid transporter 1
Chromosomal Location
ChrII:318266 to 320275 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All FAT1 GO evidence and references
  View Computational GO annotations for FAT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Pathways
Classical genetics
null
Large-scale survey
null
Resources
149 total interaction(s) for 101 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-Western: 4
  • Co-fractionation: 18
  • Co-purification: 1
  • PCA: 13
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 63
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 6
  • Positive Genetic: 14
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 669
Molecular Weight (Da) 77,140
Isoelectric Point (pI) 8.47
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:318266 to 320275 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-07-16 | Sequence: 2004-01-21
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2010 318266..320275 2004-07-16 2004-01-21
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000245
SUMMARY PARAGRAPH for FAT1

In order for yeast to utilize fatty acids, either as an energy source (via beta-oxidation) or for essential processes such as phospholipid biosynthesis and protein myristoylation, the fatty acids must first be converted into activated intermediates, acyl-CoAs, through thioesterification of fatty acids with coenzyme A. When fatty acids are synthesized de novo, activation is part of the process of synthesis and is accomplished by the same fatty acid synthetase complex (Fas1p-Fas2p) that initiates and elongates the fatty acid chain. However, yeast cells can also utilize exogenous, imported fatty acids, an ability that becomes essential if the fatty acid synthetase complex is inactivated by mutation or specific inhibitors such as cerulenin. These exogenous fatty acids are activated by one of five characterized yeast acyl-CoA synthetases: Faa1p, Faa2p, Faa3p, Faa4p, or Fat1p (see 6 and 7 for review).

FAT1 encodes an acyl-CoA synthetase required for both the import of long chain fatty acids (C14-C18) and the activation very long chain fatty acids (C20-C26) (1, 2, 8, 4). Disruption of FAT1 results in a decrease in the uptake of fatty acids, as measured by the import of fluorescently labeled long-chain fatty acid analogues and [3H]oleate (1, 3). fat1 deletion mutants also accumulate very long chain fatty acids (e.g. lignoceric acid (C24:0)) and exhibit decreased activation of these fatty acids (2, 8). Examination of a series of fat1 mutant alleles showed that these transport and fatty acyl-CoA synthetase defects are genetically separable and are thus independent activities (4).

faa1faa4 double deletion mutants also have severe defects in the import of long chain fatty acids, indicating that these genes play some role in transport as well (8, 5). Overexpression of FAT1 can rescue the faa1faa4 import phenotype and there is evidence of physical interaction between Fat1p and Faa1p and/or Faa4p (5). Fat1p, Faa1p and Faa4p are hypothesized to couple import and activation of exogenous fatty acids by a process called vectorial acylation, wherein fatty acids are metabolically trapped as CoA thioesters upon transport (reviewed in 7). This process is thought to require Fat1p along with either Faa1p and/or Faa4p (5).

The FAT1 gene was originally identified by similarity to murine Fatty Acid Transport Protein 1 (FATP1) (1, 8) and FATP1 expressed in S. cerevisiae can rescue fat1 phenotypes (3).

Last updated: 2010-02-11 Contact SGD

References cited on this page View Complete Literature Guide for FAT1
1) Faergeman NJ, et al.  (1997) Disruption of the Saccharomyces cerevisiae homologue to the murine fatty acid transport protein impairs uptake and growth on long-chain fatty acids. J Biol Chem 272(13):8531-8
2) Watkins PA, et al.  (1998) Disruption of the Saccharomyces cerevisiae FAT1 gene decreases very long-chain fatty acyl-CoA synthetase activity and elevates intracellular very long-chain fatty acid concentrations. J Biol Chem 273(29):18210-9
3) Dirusso CC, et al.  (2000) Murine FATP alleviates growth and biochemical deficiencies of yeast fat1Delta strains. Eur J Biochem 267(14):4422-33
4) Zou Z, et al.  (2002) Fatty acid transport in Saccharomyces cerevisiae. Directed mutagenesis of FAT1 distinguishes the biochemical activities associated with Fat1p. J Biol Chem 277(34):31062-71
5) Zou Z, et al.  (2003) Vectorial acylation in Saccharomyces cerevisiae. Fat1p and fatty acyl-CoA synthetase are interacting components of a fatty acid import complex. J Biol Chem 278(18):16414-22
6) Hettema EH and Tabak HF  (2000) Transport of fatty acids and metabolites across the peroxisomal membrane. Biochim Biophys Acta 1486(1):18-27
7) Black PN and Dirusso CC  (2007) Yeast acyl-CoA synthetases at the crossroads of fatty acid metabolism and regulation. Biochim Biophys Acta 1771(3):286-98
8) Choi JY and Martin CE  (1999) The Saccharomyces cerevisiae FAT1 gene encodes an acyl-CoA synthetase that is required for maintenance of very long chain fatty acid levels. J Biol Chem 274(8):4671-83