HMT1/YBR034C Summary Help

Standard Name HMT1 1
Systematic Name YBR034C
Alias HCP1 , ODP1 2 , RMT1 3
Feature Type ORF, Verified
Description Nuclear SAM-dependent mono- and asymmetric methyltransferase; modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. (1, 3, 4, 5, 6, 7, 8)
Name Description HnRNP MethylTransferase 1
Chromosomal Location
ChrII:305976 to 304930 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All HMT1 GO evidence and references
  View Computational GO annotations for HMT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
362 total interaction(s) for 300 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 116
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 16
  • Biochemical Activity: 24
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Negative Genetic: 123
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 47
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 7
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 348
Molecular Weight (Da) 39,786
Isoelectric Point (pI) 5.23
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:305976 to 304930 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2004-07-16 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1047 305976..304930 2004-07-16 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000238
References cited on this page View Complete Literature Guide for HMT1
1) Henry MF and Silver PA  (1996) A novel methyltransferase (Hmt1p) modifies poly(A)+-RNA-binding proteins. Mol Cell Biol 16(7):3668-78
2) Loubbardi A, et al.  (1995) Sterol uptake induced by an impairment of pyridoxal phosphate synthesis in Saccharomyces cerevisiae: cloning and sequencing of the PDX3 gene encoding pyridoxine (pyridoxamine) phosphate oxidase. J Bacteriol 177(7):1817-23
3) Gary JD, et al.  (1996) The predominant protein-arginine methyltransferase from Saccharomyces cerevisiae. J Biol Chem 271(21):12585-94
4) Shen EC, et al.  (1998) Arginine methylation facilitates the nuclear export of hnRNP proteins. Genes Dev 12(5):679-91
5) Lipson RS, et al.  (2010) Rmt1 catalyzes zinc-finger independent arginine methylation of ribosomal protein Rps2 in Saccharomyces cerevisiae. Biochem Biophys Res Commun 391(4):1658-62
6) Wong CM, et al.  (2010) Yeast arginine methyltransferase Hmt1p regulates transcription elongation and termination by methylating Npl3p. Nucleic Acids Res 38(7):2217-28
7) Chen YC, et al.  (2010) Protein Arginine Methylation Facilitates Cotranscriptional Recruitment of Pre-mRNA Splicing Factors. Mol Cell Biol 30(21):5245-56
8) Milliman EJ, et al.  (2012) Recruitment of rpd3 to the telomere depends on the protein arginine methyltransferase hmt1. PLoS One 7(8):e44656