CHS3/YBR023C Summary Help

Standard Name CHS3 1
Systematic Name YBR023C
Alias CAL1 2 , CSD2 , DIT101 , KTI2 3
Feature Type ORF, Verified
Description Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention (4, 5, 6, 7, 8)
Name Description CHitin Synthase-related 7
Chromosomal Location
ChrII:287925 to 284428 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 9 cM
Gene Ontology Annotations All CHS3 GO evidence and references
  View Computational GO annotations for CHS3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 68 genes
Classical genetics
reduction of function
Large-scale survey
313 total interaction(s) for 174 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 11
  • Co-purification: 1
  • PCA: 15
  • Reconstituted Complex: 1
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Negative Genetic: 103
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 56
  • Synthetic Lethality: 97
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 1,165
Molecular Weight (Da) 131,600
Isoelectric Point (pI) 6.68
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:287925 to 284428 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 9 cM
Last Update Coordinates: 2004-07-16 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3498 287925..284428 2004-07-16 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000227
References cited on this page View Complete Literature Guide for CHS3
1) Breitenbach, M.  (1992) Personal Communication, Mortimer Map Edition 11
2) Takita MA and Castilho-Valavicius B  (1993) Absence of cell wall chitin in Saccharomyces cerevisiae leads to resistance to Kluyveromyces lactis killer toxin. Yeast 9(6):589-98
3) Butler AR, et al.  (1991) Intracellular expression of Kluyveromyces lactis toxin gamma subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant. Yeast 7(6):617-25
4) Ziman M, et al.  (1996) Chs1p and Chs3p, two proteins involved in chitin synthesis, populate a compartment of the Saccharomyces cerevisiae endocytic pathway. Mol Biol Cell 7(12):1909-19
5) Shaw JA, et al.  (1991) The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J Cell Biol 114(1):111-23
6) Pammer M, et al.  (1992) DIT101 (CSD2, CAL1), a cell cycle-regulated yeast gene required for synthesis of chitin in cell walls and chitosan in spore walls. Yeast 8(12):1089-99
7) Cid VJ, et al.  (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59(3):345-86
8) Starr TL, et al.  (2012) Sorting Signals That Mediate Traffic of Chitin Synthase III between the TGN/Endosomes and to the Plasma Membrane in Yeast. PLoS One 7(10):e46386