ROX3/YBL093C Summary Help

Standard Name ROX3
Systematic Name YBL093C
Alias NUT3 1 , SSN7 , MED19 2 , SSX2 3
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme (4, 5)
Name Description Repressor Of hypoXic genes
Chromosomal Location
ChrII:44918 to 44256 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ROX3 GO evidence and references
  View Computational GO annotations for ROX3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Resources
Classical genetics
null
Large-scale survey
conditional
null
overexpression
Resources
126 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 67
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Co-purification: 5
  • PCA: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 15
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Positive Genetic: 6
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 220
Molecular Weight (Da) 24,857
Isoelectric Point (pI) 6.29
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:44918 to 44256 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..663 44918..44256 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000189
References cited on this page View Complete Literature Guide for ROX3
1) Tabtiang RK and Herskowitz I  (1998) Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol 18(8):4707-18
2) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3) Li L, et al.  (2005) Genetic interactions between mediator and the late G1-specific transcription factor Swi6 in Saccharomyces cerevisiae. Genetics 171(2):477-88
4) Gustafsson CM, et al.  (1997) Identification of Rox3 as a component of mediator and RNA polymerase II holoenzyme. J Biol Chem 272(1):48-50
5) Myers LC, et al.  (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54