NUP170/YBL079W Summary Help

Standard Name NUP170 1
Systematic Name YBL079W
Alias NLE3
Feature Type ORF, Verified
Description Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and see Summary Paragraph)
Name Description NUclear Pore 1
Chromosomal Location
ChrII:75259 to 79767 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NUP170 GO evidence and references
  View Computational GO annotations for NUP170
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
170 total interaction(s) for 116 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 41
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • Co-purification: 2
  • Reconstituted Complex: 3

Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 1
  • Negative Genetic: 50
  • Phenotypic Enhancement: 3
  • Positive Genetic: 26
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 15
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,502
Molecular Weight (Da) 169,473
Isoelectric Point (pI) 6.04
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:75259 to 79767 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4509 75259..79767 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000175
SUMMARY PARAGRAPH for NUP170

NUP170 encodes a nuclear pore protein (1, 11). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 11, 12, 13, 14). The structure of the vertebrate NPC has been studied extensively; recent reviews include 15, 16, 17, and 18. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (19, 20). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 11, 12, 21, 22, 23). Nup170p is one of the most abundant yeast nucleoporins (1). Nup170p interacts physically with other abundant yeast nucleoporins, Nic96p, Pom152p, Nup157p, and Nup188p (24, 11). These abundant nucleoporins may form the structural core of the NPC (11). Also, Nup170p forms a complex with the nucleoporins Nup53p and Asm4p (25). The complex is found on both the nuclear and cytoplasmic faces of the NPC, and associates with Pse1p, a member of a protein family implicated in nuclear protein import. Nup157p and Nup170p are very similar to each other and to a mammalian nucleoporin, Nup155 (1, 3, 11). NUP170 is not essential, but nup170 mutations are synthetically lethal with mutations in several other nucleoporin genes (26, 11).

Last updated: 1999-08-12 Contact SGD

References cited on this page View Complete Literature Guide for NUP170
1) Aitchison JD, et al.  (1995) Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p. J Cell Biol 131(5):1133-48
2) Shulga N, et al.  (2000) Yeast nucleoporins involved in passive nuclear envelope permeability. J Cell Biol 149(5):1027-38
3) Kenna MA, et al.  (1996) Yeast N1e3p/Nup170p is required for normal stoichiometry of FG nucleoporins within the nuclear pore complex. Mol Cell Biol 16(5):2025-36
4) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
5) Kerscher O, et al.  (2001) Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation. Genetics 157(4):1543-53
6) Iouk T, et al.  (2002) The yeast nuclear pore complex functionally interacts with components of the spindle assembly checkpoint. J Cell Biol 159(5):807-19
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Makio T, et al.  (2009) The nucleoporins Nup170p and Nup157p are essential for nuclear pore complex assembly. J Cell Biol 185(3):459-73
9) Fernandez-Martinez J and Rout MP  (2009) Nuclear pore complex biogenesis. Curr Opin Cell Biol 21(4):603-12
10) Meinema AC, et al.  (2011) Long unfolded linkers facilitate membrane protein import through the nuclear pore complex. Science 333(6038):90-3
11) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
12) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
13) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
14) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
15) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
16) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
17) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
18) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
19) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
20) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
21) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
22) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
23) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
24) Nehrbass U, et al.  (1996) The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex. J Cell Biol 133(6):1153-62
25) Marelli M, et al.  (1998) Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p. J Cell Biol 143(7):1813-30
26) Tcheperegine SE, et al.  (1999) Topology and functional domains of the yeast pore membrane protein Pom152p. J Biol Chem 274(8):5252-8