EDE1/YBL047C Summary Help

Standard Name EDE1 1
Systematic Name YBL047C
Alias BUD15 2
Feature Type ORF, Verified
Description Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death (1, 3, 4 and see Summary Paragraph)
Name Description EH Domains and Endocytosis 1
Chromosomal Location
ChrII:132040 to 127895 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All EDE1 GO evidence and references
  View Computational GO annotations for EDE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 86 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
269 total interaction(s) for 160 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 4
  • Biochemical Activity: 8
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 18

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 130
  • Phenotypic Enhancement: 15
  • Phenotypic Suppression: 10
  • Positive Genetic: 11
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 17
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,381
Molecular Weight (Da) 150,782
Isoelectric Point (pI) 4.44
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:132040 to 127895 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4146 132040..127895 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000143
SUMMARY PARAGRAPH for EDE1

Ede1p is a key endocytic protein that exhibits a cortical patch membrane localization pattern, and is involved in a network of interactions with other endocytic proteins that localize to membranes. Ede1p comprises a multimodular domain structure including three EH domains, a proline-rich region, coiled coils, and a ubiquitin-associated domain (UBA) (3, 1). The UBA binds membranes in a ubiquitin-dependent manner, and this recruitment of Ede1p to membranes is inhibited by free ubiquitin. Ede1p may also bind ubiquitinated membrane-associated proteins. Both deletion of EDE1 and inactivation of the Ent1p ubiquitin interaction motif are required for a significant endocytic defect (3).

Last updated: 2004-03-28 Contact SGD

References cited on this page View Complete Literature Guide for EDE1
1) Gagny B, et al.  (2000) A novel EH domain protein of Saccharomyces cerevisiae, Ede1p, involved in endocytosis. J Cell Sci 113 ( Pt 18)():3309-19
2) Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
3) Aguilar RC, et al.  (2003) The yeast Epsin Ent1 is recruited to membranes through multiple independent interactions. J Biol Chem 278(12):10737-43
4) Kim H, et al.  (2012) Vacuolar H+-ATPase (V-ATPase) promotes vacuolar membrane permeabilization and nonapoptotic death in stressed yeast. J Biol Chem 287(23):19029-39