SLA1/YBL007C Summary

SLA1/YBL007C Summary

Standard Name	SLA1 1
Systematic Name	YBL007C
Feature Type	ORF, Verified
Description	Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains (2, 3, 4, 5)
Name Description	Synthetic Lethal with ABP1 1

Chromosomal Location	ChrII:216366 to 212632 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All SLA1 GO evidence and references

	View Computational GO annotations for SLA1
Molecular Function
Manually curated	protein binding, bridging (IPI) ubiquitin binding (IDA)
Biological Process
Manually curated	actin cortical patch assembly (TAS) endocytosis (IMP, IPI) fungal-type cell wall organization (TAS)
Cellular Component
Manually curated	actin cortical patch (IDA) cell cortex (IDA) nucleus (IDA, IMP)
High-throughput	mating projection tip (IDA)

Mutant phenotypes All SLA1 Phenotype evidence and references

Classical genetics
null	cell shape: abnormal tellurium atom accumulation: increased endocytosis: decreased filamentous growth: abnormal metal resistance: decreased resistance to ethanol: decreased resistance to sirolimus: decreased resistance to tellurite: decreased
overexpression	viability: decreased
unspecified	endocytosis: decreased Wsc1p-GFP distribution: abnormal
Large-scale survey
null	acid pH resistance: decreased bud morphology: abnormal budding pattern: abnormal cell shape: abnormal glutathione excretion: increased chitin deposition: increased competitive fitness: decreased endocytosis: decreased heat sensitivity: increased hyperosmotic stress resistance: decreased mitochondrial genome maintenance: abnormal oxidative stress resistance: decreased resistance to mefloquine: decreased resistance to boric acid: decreased resistance to mycophenolic acid: decreased resistance to methyl methanesulfonate: decreased resistance to fluconazole: decreased resistance to fenpropimorph: decreased resistance to caffeine: decreased resistance to bleomycin: decreased resistance to 1,4-dithiothreitol: decreased resistance to tunicamycin: decreased resistance to quinine: decreased resistance to cycloheximide: decreased resistance to arsenite(3-): decreased resistance to ethanol: decreased resistance to tirapazamine: decreased resistance to propan-1-ol: decreased resistance to methanol: decreased resistance to hydroxyurea: decreased resistance to monensin: decreased resistance to Calcofluor White: decreased resistance to tunicamycin: increased resistance to sirolimus: increased resistance to streptomycin: decreased resistance to doxorubicin: decreased resistance to calcium dichloride: decreased respiratory growth: absent respiratory growth: decreased rate toxin resistance: decreased transposable element transposition: decreased utilization of carbon source: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SLA1 Interaction evidence and references

	613 total interaction(s) for 376 unique genes/features.
Physical Interactions	Affinity Capture-MS: 44 Affinity Capture-RNA: 2 Affinity Capture-Western: 23 Biochemical Activity: 17 Co-fractionation: 1 Co-localization: 1 FRET: 2 PCA: 7 Protein-peptide: 1 Reconstituted Complex: 14 Two-hybrid: 140
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 5 Dosage Rescue: 1 Negative Genetic: 250 Phenotypic Enhancement: 6 Phenotypic Suppression: 2 Positive Genetic: 25 Synthetic Growth Defect: 28 Synthetic Lethality: 42 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SLA1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:216366 to 212632 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3735	216366..212632	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000103

References cited on this page View Complete Literature Guide for SLA1

1)	Holtzman DA, et al. (1993) Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J Cell Biol 122(3):635-44
2)	Pruyne D and Bretscher A (2000) Polarization of cell growth in yeast. J Cell Sci 113 ( Pt 4):571-85
3)	Howard JP, et al. (2002) Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis. J Cell Biol 157(2):315-26
4)	Warren DT, et al. (2002) Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics. J Cell Sci 115(Pt 8):1703-15
5)	Gardiner FC, et al. (2007) Nucleocytoplasmic trafficking is required for functioning of the adaptor protein sla1p in endocytosis. Traffic 8(4):347-58