RFA1/YAR007C Summary 
RFA1 BASIC INFORMATION
| Standard Name | RFA1 |
|---|---|
| Systematic Name | YAR007C |
| Alias | BUF2 , FUN3 1 , RPA1 2 |
| Feature Type | ORF, Verified |
| Description | Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination (3, 4, 5 and see Summary Paragraph) 
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| Name Description | Replication Factor A 3 |
| GO Annotations | All RFA1 GO evidence and references |
|---|---|
| View Computational GO annotations for RFA1 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated |
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| Cellular Component | |
| Manually curated |
| Interactions | RFA1 All interactions details and references |
|---|---|
| 166 total interaction(s) for 96 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
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| Primary SGDID | S000000065 |
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RFA1 RESOURCES
| SGD ORF map | GBrowse |
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for RFA1
SUMMARY PARAGRAPH for RFA1
RFA1, RFA2, and RFA3 encode the three subunits of the heterotrimeric complex Replication protein A (RPA). RPA is a highly conserved single-stranded DNA (ssDNA) binding protein that removes secondary structure from ssDNA. RPA plays a key role in DNA metabolic pathways that have a ssDNA intermediate such as replication, recombination, transcription, checkpoints, telomere maintenance, and DNA repair (reviewed in 6 and 7). Some specific instances of RPA function in yeast include loading the telomerase component Est1p onto telomeres (8), enabling the efficient assembly of the Rad51p presynaptic filament (9, 10), and providing specificity of Rad17p/Mec3p/Ddc1p loading at 5'-junctions (11).
RPA binds ssDNA with high affinity and has no sequence specificity but does preferentially bind to pyrimidines over purines (5, 12). RPA interaction with DNA is mediated by binding domains in Rfa1p and Rfa2p (13). In response to DNA damage, Rfa1p and Rfa2p are phosphorylated by overlapping mechanisms that involve the kinases Mec1p and Tel1p (14, 15, 16). These same kinases also mediate Rfa2p phosphorylation during the normal cell cycle and during meiosis (14, 17).
All three RFA subunits are essential for viability in yeast, but reduction of function mutations in RPA result in various defects such as sensitivity to DNA damaging agents, altered mitotic and meiotic recombination, failure in mating type switching, severe telomere shortening, and chromosomal instability (8, 10 and reviewed in 7, 18). Gross chromosomal rearrangements found in rfa1 mutants are similar to those seen in some human cancers, and corresponding mutations in mice lead to tumor development (19 and references therein).
Last updated: 2007-08-09
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RFA1]
| 1) | Steensma HY, et al. (1987) Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and analysis of the CEN1-ADE1-CDC15 region. Mol Cell Biol 7(1):410-9 |
| 2) | Heyer WD, et al. (1990) An essential Saccharomyces cerevisiae single-stranded DNA binding protein is homologous to the large subunit of human RP-A. EMBO J 9(7):2321-9 |
| 3) | Brill SJ and Stillman B (1991) Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase. Genes Dev 5(9):1589-600 |
| 4) | Longhese MP, et al. (1994) Replication factor A is required in vivo for DNA replication, repair, and recombination. Mol Cell Biol 14(12):7884-90 |
| 5) | Alani E, et al. (1992) Characterization of DNA-binding and strand-exchange stimulation properties of y-RPA, a yeast single-strand-DNA-binding protein. J Mol Biol 227(1):54-71 |
| 6) | Sung P, et al. (2003) Rad51 recombinase and recombination mediators. J Biol Chem 278(44):42729-32 |
| 7) | Symington LS (2002) Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev 66(4):630-70, table of contents |
| 8) | Schramke V, et al. (2004) RPA regulates telomerase action by providing Est1p access to chromosome ends. Nat Genet 36(1):46-54 |
| 9) | Hays SL, et al. (1998) Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA. Mol Cell Biol 18(7):4400-6 |
| 10) | Kantake N, et al. (2003) The recombination-deficient mutant RPA (rfa1-t11) is displaced slowly from single-stranded DNA by Rad51 protein. J Biol Chem 278(26):23410-7 |
| 11) | Majka J, et al. (2006) Replication protein A directs loading of the DNA damage checkpoint clamp to 5'-DNA junctions. J Biol Chem 281(38):27855-61 |
| 12) | Kim C, et al. (1992) Binding properties of replication protein A from human and yeast cells. Mol Cell Biol 12(7):3050-9 |
| 13) | Brill SJ and Bastin-Shanower S (1998) Identification and characterization of the fourth single-stranded-DNA binding domain of replication protein A. Mol Cell Biol 18(12):7225-34 |
| 14) | Brush GS, et al. (1996) The ATM homologue MEC1 is required for phosphorylation of replication protein A in yeast. Proc Natl Acad Sci U S A 93(26):15075-80 |
| 15) | Brush GS and Kelly TJ (2000) Phosphorylation of the replication protein A large subunit in the Saccharomyces cerevisiae checkpoint response. Nucleic Acids Res 28(19):3725-32 |
| 16) | Kim HS and Brill SJ (2003) MEC1-dependent phosphorylation of yeast RPA1 in vitro. DNA Repair (Amst) 2(12):1321-35 |
| 17) | Brush GS, et al. (2001) Replication protein A is sequentially phosphorylated during meiosis. Nucleic Acids Res 29(23):4808-17 |
| 18) | Sung P, et al. (2000) Recombination factors of Saccharomyces cerevisiae. Mutat Res 451(1-2):257-75 |
| 19) | Wang Y, et al. (2005) Mutation in Rpa1 results in defective DNA double-strand break repair, chromosomal instability and cancer in mice. Nat Genet 37(7):750-5 |
