GEM1/YAL048C Summary Help

Standard Name GEM1 1
Systematic Name YAL048C
Alias GON1
Feature Type ORF, Verified
Description Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases (1, 2, 3, 4 and see Summary Paragraph)
Name Description GTPase EF-hand protein of Mitochondria 1
Chromosomal Location
ChrI:54789 to 52801 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All GEM1 GO evidence and references
  View Computational GO annotations for GEM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
144 total interaction(s) for 122 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 3
  • Negative Genetic: 62
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 56
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 662
Molecular Weight (Da) 75,149
Isoelectric Point (pI) 5.85
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrI:54789 to 52801 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1989 54789..52801 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000046

Gem1p (encoded by YAL048C) is a member of the evolutionarily conserved mitochondrial Rho (Miro) subgroup of Rho GTPases (5). A tail-anchored outer mitochondrial membrane protein, Gem1p contains two GTPase domains flanking a pair of calcium-binding EF-hand motifs which are exposed to the cytoplasm and required for function. Gem1p is a subunit of the ERMES complex that links the ER and mitochondria (3, 4). Cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria (1). They also contain fewer, and larger, ERMES complex foci than normal (4). Overexpression of a mutant human Miro protein causes increased apoptotic activity in cultured cells (5), but Gem1p is not required for pheromone-induced yeast cell death.

Last updated: 2012-01-18 Contact SGD

References cited on this page View Complete Literature Guide for GEM1
1) Frederick RL, et al.  (2004) Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway. J Cell Biol 167(1):87-98
2) Wolff AM, et al.  (1999) The open reading frame YAL048c affects the secretion of proteinase A in S. cerevisiae. Yeast 15(5):427-34
3) Stroud DA, et al.  (2011) Composition and topology of the endoplasmic reticulum-mitochondria encounter structure. J Mol Biol 413(4):743-50
4) Kornmann B, et al.  (2011) The conserved GTPase Gem1 regulates endoplasmic reticulum-mitochondria connections. Proc Natl Acad Sci U S A 108(34):14151-6
5) Fransson A, et al.  (2003) Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. J Biol Chem 278(8):6495-502