SPC72/YAL047C Summary

SPC72 BASIC INFORMATION

Standard Name	SPC72
Systematic Name	YAL047C
Alias	LDB4
Feature Type	ORF, Verified
Description	Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization (1, 2, 3)
Name Description	Spindle Pole Component

GO Annotations	All SPC72 GO evidence and references
	View Computational GO annotations for SPC72
Molecular Function
Manually curated	structural constituent of cytoskeleton (IPI)
Biological Process
Manually curated	microtubule nucleation (IMP) mitotic cell cycle spindle orientation checkpoint (IGI, IPI) mitotic sister chromatid segregation (IMP) mitotic spindle elongation (IMP) nuclear migration along microtubule (IMP)
Cellular Component
Manually curated	outer plaque of spindle pole body (IDA)

Mutant Phenotype	All SPC72 Phenotype details and references
Classical genetics
null	metal resistance: decreased
reduction of function	budding index: abnormal
Large-scale survey
null	alkaline pH resistance: decreased bud morphology: abnormal competitive fitness: decreased heat sensitivity: increased resistance to sulfanilamide: decreased resistance to mycophenolic acid: decreased resistance to hydroxyurea: decreased resistance to doxorubicin: decreased resistance to ethanol: decreased resistance to methanol: decreased resistance to propan-1-ol: decreased respiratory growth: absent viable

Interactions	SPC72 All interactions details and references
	75 total interaction(s) for 36 unique genes/features.
Physical Interactions	Affinity Capture-MS: 11 Affinity Capture-Western: 13 Biochemical Activity: 2 Co-localization: 2 Co-purification: 1 FRET: 2 Reconstituted Complex: 8 Two-hybrid: 20
Genetic Interactions	Dosage Rescue: 3 Synthetic Growth Defect: 10 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 2

Sequence Information

ChrI:56858 to 54990 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-07-20 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1869	56858..54990	2004-07-20	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000045

SPC72 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SPC72

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SPC72]

1)	Knop M and Schiebel E (1998) Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation. EMBO J 17(14):3952-67
2)	Wigge PA, et al. (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol 141(4):967-77
3)	Hoepfner D, et al. (2002) Reorientation of mispositioned spindles in short astral microtubule mutant spc72Delta is dependent on spindle pole body outer plaque and Kar3 motor protein. Mol Biol Cell 13(4):1366-80

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