CLN3/YAL040C Summary Help

CLN3 BASIC INFORMATION

Standard Name CLN3 1
Systematic Name YAL040C
Alias DAF1 2 , FUN10 3 , WHI1 4
Feature Type ORF, Verified
Description G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis (4, 5, 6 and see Summary Paragraph)
Name Description CycLiN
GO Annotations All CLN3 GO evidence and references
    View Computational GO annotations for CLN3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All CLN3 Phenotype details and references
Classical genetics
activation
null
overexpression
repressible
unspecified
Large-scale survey
null
Interactions CLN3 All interactions details and references
183 total interaction(s) for 115 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Biochemical Activity: 6
  • Protein-RNA: 1
  • Two-hybrid: 2
Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 17
  • Phenotypic Enhancement: 64
  • Phenotypic Suppression: 34
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 19
  • Synthetic Rescue: 20
Sequence Information
ChrI:67521 to 65779 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -56 cM
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1743 67521..65779 2004-07-20 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000038

CLN3 RESOURCES

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Expression Summary histogram

ADDITIONAL INFORMATION for CLN3

SUMMARY PARAGRAPH for CLN3

CLN3 encodes a G1 cyclin involved in regulation of the cell cycle. Progression through the cell cycle is a carefully regulated process that is conserved throughout eukaryotes. Periodic activation of cyclin-dependent kinases (CDKs) are required for this process; the critical CDK involved in cell cycle progression in yeast is Cdc28p.

Cyclins are the regulatory subunits that activate CDKs at the appropriate time in the cell cycle; they were named for their cyclical accumulation during particular phases of the cell cycle (7). Distinct CDK-cyclin complexes are required for progression through different stages of the cell cycle (8). CLN1, CLN2, and CLN3 encode the yeast cyclins involved in the G1 to S phase transition.

While transcription of CLN1 and CLN2 is regulated by the cell cycle, CLN3 transcription is not. Instead, Cln3p is regulated post-translationally (6, 5). Cln3p is an unstable protein that contains several PEST motifs, which are sequences rich in proline, glutamic acid, serine, and threonine and are found in many other unstable proteins (6, 5). Cln3p is phosphorylated by Cdc28p; this phosphorylation may make the Cln3 protein less stable (5). The chaperone Ydj1p is required for the phosphorylation and degradation of Cln3p (9). In addition to being carefully regulated itself, Cln3p also plays a role in regulating the transcriptional activation of the other G1 cyclins, CLN1 and CLN2 (10, 11). An excellent review by Lew et al. describes cell cycle control in S. cerevisiae in detail (8).

Last updated: 1999-12-17

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CLN3]

1) Tokiwa, G.  (1992) Personal Communication, Mortimer Map Edition 11
2) Cross FR  (1988) DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae. Mol Cell Biol 8(11):4675-84
3) Coleman KG, et al.  (1986) Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome. Mol Cell Biol 6(12):4516-25
4) Nash R, et al.  (1988) The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is a cyclin homolog. EMBO J 7(13):4335-46
5) Cross FR and Blake CM  (1993) The yeast Cln3 protein is an unstable activator of Cdc28. Mol Cell Biol 13(6):3266-71
6) Tyers M, et al.  (1992) The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation. EMBO J 11(5):1773-84
7) Evans T, et al.  (1983) Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division. Cell 33(2):389-96
8) Lew DJ, et al.  (1997) "Cell cycle control in Saccharomyces cerevisiae." Pp. 607-695 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
9) Yaglom JA, et al.  (1996) The molecular chaperone Ydj1 is required for the p34CDC28-dependent phosphorylation of the cyclin Cln3 that signals its degradation. Mol Cell Biol 16(7):3679-84
10) Stuart D and Wittenberg C  (1995) CLN3, not positive feedback, determines the timing of CLN2 transcription in cycling cells. Genes Dev 9(22):2780-94
11) Dirick L, et al.  (1995) Roles and regulation of Cln-Cdc28 kinases at the start of the cell cycle of Saccharomyces cerevisiae. EMBO J 14(19):4803-13