VPS8/YAL002W Summary Help

VPS8 BASIC INFORMATION

Standard Name VPS8 1, 2
Systematic Name YAL002W
Alias FUN15 3 , VPT8 4 , VPL8 5
Feature Type ORF, Verified
Description Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the CPY sorting receptor; contains RING finger motif (6, 7, 8, 9)
Name Description Vacuolar Protein Sorting 1, 2
GO Annotations All VPS8 GO evidence and references
    View Computational GO annotations for VPS8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All VPS8 Phenotype details and references
Classical genetics
null
reduction of function
Large-scale survey
null
Interactions VPS8 All interactions details and references
161 total interaction(s) for 130 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-Western: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 80
  • Phenotypic Suppression: 18
  • Synthetic Growth Defect: 21
  • Synthetic Lethality: 14
  • Synthetic Rescue: 3

Sequence Information
ChrI:143709 to 147533 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 2004-01-14
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3825 143709..147533 2004-07-20 2004-01-14
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000002

VPS8 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for VPS8]

1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Clark MW, et al.  (1994) Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region. Yeast 10(4):535-41
4) Bankaitis VA, et al.  (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
5) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
6) Horazdovsky BF, et al.  (1996) A novel RING finger protein, Vps8p, functionally interacts with the small GTPase, Vps21p, to facilitate soluble vacuolar protein localization. J Biol Chem 271(52):33607-15
7) Luo W and Chang A  (1997) Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant. J Cell Biol 138(4):731-46
8) Chen YJ and Stevens TH  (1996) The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae. Eur J Cell Biol 70(4):289-97
9) Peplowska K, et al.  (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50