| YHR079C |
IRE1 |
Serine-threonine kinase and endoribonuclease |
Biochemical Activity |
physical interactions |
Bait |
BioGRID |
high-throughput |
Phosphorylation |
|
32P incorporation on protein chip |
Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
| YDL003W |
MCD1 |
Essential alpha-kleisin subunit of the cohesin complex |
Affinity Capture-MS |
physical interactions |
Bait |
BioGRID |
high-throughput |
|
|
|
Graumann J, et al. (2004) Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast. Mol Cell Proteomics 3(3):226-37 |
| YGL122C |
NAB2 |
Nuclear polyadenylated RNA-binding protein |
Affinity Capture-RNA |
physical interactions |
Bait |
BioGRID |
high-throughput |
|
|
High Throughput: The experiment involved high-throughput sequencing of the RNAs that co-precipitated with TAP-tagged Nab2 (Nab2-TAP) |
Batisse J, et al. (2009) Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structure. J Biol Chem 284(50):34911-7 |
| YPL031C |
PHO85 |
Cyclin-dependent kinase |
Biochemical Activity |
physical interactions |
Bait |
BioGRID |
high-throughput |
Phosphorylation |
|
32P incorporation on protein chip |
Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
| YFR004W |
RPN11 |
Metalloprotease subunit of the 19S regulatory particle of the 26S pro |
Affinity Capture-MS |
physical interactions |
Bait |
BioGRID |
high-throughput |
|
|
Proteasome interacting proteins (PIPs) were identified using QTAX (quantitative analysis of tandem affinity purified in vivo cross-linked (X) protein complexes) |
Kaake RM, et al. (2010) Characterization of cell cycle specific protein interaction networks of the yeast 26S proteasome complex by the QTAX strategy. J Proteome Res 9(4):2016-29 |
| YDR328C |
SKP1 |
Evolutionarily conserved kinetochore protein |
Affinity Capture-MS |
physical interactions |
Bait |
BioGRID |
high-throughput |
|
|
The ubiquitin conjugates were detected under denaturing conditions that inhibit ubiquitin hydrolase activity |
Tagwerker C, et al. (2006) A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking. Mol Cell Proteomics 5(4):737-48 |
| YBL088C |
TEL1 |
Protein kinase primarily involved in telomere length regulation |
Dosage Growth Defect |
genetic interactions |
Bait |
BioGRID |
high-throughput |
|
vegetative growth:decreased |
score threshold >=0 |
Sharifpoor S, et al. (2012) Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs. Genome Res 22(4):791-801 |
| YNL154C |
YCK2 |
Palmitoylated plasma membrane-bound casein kinase I isoform |
Biochemical Activity |
physical interactions |
Bait |
BioGRID |
high-throughput |
Phosphorylation |
|
32P incorporation on protein chip |
Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |