Restriction Fragments for ATG29/YPL166W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ATG29/YPL166W on chromosome XVI from coordinates 237338 to 237979.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.24019.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 11 + TspDTI ATGAA 22 20 . . 13 18 + TatI WGTACW 13 17 . . 14 17 + Csp6I GTAC 14 16 . . 14 17 + RsaI GTAC 15 15 . . 22 26 + Tsp4CI* ACNGT 24 23 . . 41 44 + MseI TTAA 41 43 . . 51 55 - BsmAI GTCTC 45 49 . . 51 56 - Eco31I GGTCTC 45 49 . . 62 66 + TfiI GAWTC 62 65 . . 62 66 + HinfI GANTC 62 65 . . 69 74 + XhoII RGATCY 69 73 . . 69 74 + NlaIV GGNNCC 71 71 . . 69 74 + BamHI GGATCC 69 73 . . 69 73 + BinI* GGATC 77 78 . . 70 73 + DpnI GATC 71 71 . . 70 73 + BstKTI GATC 72 70 . . 70 73 + MboI GATC 69 73 . . 73 76 + MnlI CCTC 83 82 . . 70 74 - BinI* GGATC 64 65 . . 79 84 + ApoI RAATTY 79 83 . . 80 83 + TspEI AATT 79 83 . . 110 113 + SetI ASST 113 109 . . 110 113 + CviJI RGCY 111 111 . . 110 113 + AluI AGCT 111 111 . . 118 128 + BaeI ACNNNNGTAYC 107 102 140 135 129 134 + ApoI RAATTY 129 133 . . 130 133 + TspEI AATT 129 133 . . 136 139 + TspEI AATT 135 139 . . 148 151 + DpnI GATC 149 149 . . 148 151 + BstKTI GATC 150 148 . . 148 151 + MboI GATC 147 151 . . 165 170 + ApoI RAATTY 165 169 . . 166 169 + TspEI AATT 165 169 . . 177 182 + SspI AATATT 179 179 . . 193 202 + XmnI GAANNNNTTC 197 197 . . 193 198 + ApoI RAATTY 193 197 . . 194 197 + TspEI AATT 193 197 . . 203 206 + MseI TTAA 203 205 . . 221 224 + TspEI AATT 220 224 . . 237 241 + DdeI CTNAG 237 240 . . 242 245 + SetI ASST 245 241 . . 242 245 + CviJI RGCY 243 243 . . 242 245 + AluI AGCT 243 243 . . 256 261 + MfeI CAATTG 256 260 . . 257 260 + TspEI AATT 256 260 . . 273 278 + XhoII RGATCY 273 277 . . 273 278 + BglII AGATCT 273 277 . . 274 277 + DpnI GATC 275 275 . . 274 277 + BstKTI GATC 276 274 . . 274 277 + MboI GATC 273 277 . . 276 281 + Hpy178III* TCNNGA 277 279 . . 277 282 + SmlI CTYRAG 277 281 . . 277 282 + Bce83I* CTTGAG 298 296 . . 294 299 + BclI TGATCA 294 298 . . 295 298 + DpnI GATC 296 296 . . 295 298 + BstKTI GATC 297 295 . . 295 298 + MboI GATC 294 298 . . 305 309 + TspDTI ATGAA 320 318 . . 307 311 - Hin4II* CCTTC 301 300 . . 311 315 + FokI GGATG 324 328 . . 311 315 + BseGI GGATG 317 315 . . 316 320 + Hpy188I TCNGA 318 317 . . 322 325 + MseI TTAA 322 324 . . 364 367 + MseI TTAA 364 366 . . 370 375 + BciVI GTATCC 381 380 . . 374 377 + AciI CCGC 374 376 . . 376 381 + Cac8I GCNNGC 378 378 . . 379 382 + CviJI RGCY 380 380 . . 383 389 + TspRI CASTGNN 389 380 . . 389 394 + DsaI* CCRYGG 389 393 . . 389 394 + SecI* CCNNGG 389 393 . . 391 395 + TspGWI ACGGA 406 404 . . 403 407 + TfiI GAWTC 403 406 . . 403 407 + HinfI GANTC 403 406 . . 406 410 + Hpy188I TCNGA 408 407 . . 407 412 + Eco57MI CTGRAG 428 426 . . 407 412 + Eco57I CTGAAG 428 426 . . 409 413 + MboII GAAGA 421 420 . . 418 422 - BsmAI GTCTC 412 416 . . 427 431 + MaeIII GTNAC 426 431 . . 436 439 - MnlI CCTC 429 428 . . 441 445 + MaeIII GTNAC 440 445 . . 452 455 + CviRI* TGCA 453 453 . . 464 469 + ApoI RAATTY 464 468 . . 465 468 + TspEI AATT 464 468 . . 490 494 + Hpy188I TCNGA 492 491 . . 506 509 + CviJI RGCY 507 507 . . 518 521 + TspEI AATT 517 521 . . 528 532 + FokI GGATG 541 545 . . 528 532 + BseGI GGATG 534 532 . . 529 533 - BccI CCATC 523 524 . . 535 540 + VspI ATTAAT 536 538 . . 536 539 + MseI TTAA 536 538 . . 554 557 - AciI CCGC 554 556 . . 562 566 + Hpy188I TCNGA 564 563 . . 568 578 + Hin4I GAYNNNNNVTC 559 554 591 586 568 578 - Hin4I GAYNNNNNVTC 591 586 559 554 580 583 + TaqI TCGA 580 582 . . 577 581 - MboII GAAGA 569 568 . . 583 586 + SetI ASST 586 582 . . 583 586 + CviJI RGCY 584 584 . . 583 586 + AluI AGCT 584 584 . . 585 590 + SmlI CTYRAG 585 589 . . 585 590 + AflII CTTAAG 585 589 . . 586 589 + MseI TTAA 586 588 . . 610 614 + MboII GAAGA 622 621 . . 606 611 - MmeI TCCRAC 587 585 . . 615 620 + HaeII RGCGCY 619 615 . . 615 620 + Eco47III AGCGCT 617 617 . . 616 619 + Hin6I GCGC 616 618 . . 616 619 + GlaI GCGC 617 617 . . 616 619 + HhaI GCGC 618 616 . . 632 635 + CviRI* TGCA 633 633 . . 635 638 + TspEI AATT 634 638 . . 638 642 + Hpy188I TCNGA 640 639 . . #--------------------------------------- # # # AciI: # [CCGC] # 88 180 374 # # AflII: # [CTTAAG] # 57 585 # # AluI: # [AGCT] # 58 111 132 341 # # ApoI: # [RAATTY] # 28 36 50 79 178 271 # # BaeI: # [ACNNNNGTAYC] # 33 107 502 # # BamHI: # [GGATCC] # 69 573 # # BccI: # [CCATC] # 119 523 # # Bce83I*: # [CTTGAG] # 298 344 # # BciVI: # [GTATCC] # 261 381 # # BclI: # [TGATCA] # 294 348 # # BglII: # [AGATCT] # 273 369 # # BinI*: # [GGATC] # 13 64 565 # # BseGI: # [GGATG] # 108 217 317 # # BsmAI: # [GTCTC] # 45 230 367 # # BstKTI: # [GATC] # 21 72 78 126 345 # # Cac8I: # [GCNNGC] # 264 378 # # Csp6I: # [GTAC] # 14 628 # # CviJI: # [RGCY] # 58 77 111 127 132 137 # # CviRI*: # [TGCA] # 9 180 453 # # DdeI: # [CTNAG] # 237 405 # # DpnI: # [GATC] # 21 71 78 126 346 # # DsaI*: # [CCRYGG] # 253 389 # # Eco31I: # [GGTCTC] # 45 597 # # Eco47III: # [AGCGCT] # 25 617 # # Eco57I: # [CTGAAG] # 214 428 # # Eco57MI: # [CTGRAG] # 214 428 # # FokI: # [GGATG] # 101 217 324 # # GlaI: # [GCGC] # 25 617 # # HaeII: # [RGCGCY] # 23 619 # # HhaI: # [GCGC] # 24 618 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 51 559 # # Hin4II*: # [CCTTC] # 301 341 # # Hin6I: # [GCGC] # 26 616 # # HinfI: # [GANTC] # 62 239 341 # # Hpy178III*: # [TCNNGA] # 277 365 # # Hpy188I: # [TCNGA] # 2 72 76 84 90 318 # # MaeIII: # [GTNAC] # 14 202 426 # # MboI: # [GATC] # 21 69 78 126 348 # # MboII: # [GAAGA] # 20 53 148 421 # # MfeI: # [CAATTG] # 256 386 # # MmeI: # [TCCRAC] # 55 587 # # MnlI: # [CCTC] # 83 213 346 # # MseI: # [TTAA] # 41 42 50 56 119 162 # 172 # # NlaIV: # [GGNNCC] # 71 571 # # RsaI: # [GTAC] # 15 627 # # SecI*: # [CCNNGG] # 253 389 # # SetI: # [ASST] # 56 113 132 341 # # SmlI: # [CTYRAG] # 57 277 308 # # SspI: # [AATATT] # 179 463 # # TaqI: # [TCGA] # 62 580 # # TatI: # [WGTACW] # 13 629 # # TfiI: # [GAWTC] # 62 239 341 # # Tsp4CI*: # [ACNGT] # 24 618 # # TspDTI: # [ATGAA] # 22 298 322 # # TspEI: # [AATT] # 6 8 27 28 30 36 # 50 53 79 117 208 # # TspGWI: # [ACGGA] # 236 406 # # TspRI: # [CASTGNN] # 253 389 # # VspI: # [ATTAAT] # 106 536 # # XhoII: # [RGATCY] # 69 204 369 # # XmnI: # [GAANNNNTTC] # 197 445 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 642 # Reported_sequences: 1 # Reported_hitcount: 115 #---------------------------------------