Restriction Fragments for PSH1/YOL054W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

PSH1/YOL054W on chromosome XV from coordinates 228614 to 229834.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.29882.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 10 + Hpy99I CGWCG 10 5 . . 11 14 + TspEI AATT 10 14 . . 20 24 + Tsp4CI* ACNGT 22 21 . . 23 28 + Hpy166II GTNNAC 25 25 . . 33 44 + BstXI CCANNNNNNTGG 40 36 . . 30 34 - HphI GGTGA 22 21 . . 43 48 + NlaIV GGNNCC 45 45 . . 43 48 + HgiCI* GGYRCC 43 47 . . 40 44 - BccI CCATC 34 35 . . 47 52 + StyI CCWWGG 47 51 . . 47 52 + SecI* CCNNGG 47 51 . . 52 56 + HgaI GACGC 61 66 . . 52 57 + AcyI GRCGYC 53 55 . . 63 68 + PsiI TTATAA 65 65 . . 76 80 + TfiI GAWTC 76 79 . . 76 80 + HinfI GANTC 76 79 . . 89 99 + MwoI GCNNNNNNNGC 95 92 . . 92 96 + BccI CCATC 100 101 . . 115 118 + Csp6I GTAC 115 117 . . 115 118 + RsaI GTAC 116 116 . . 128 136 + DraIII CACNNNGTG 133 130 . . 129 132 + SetI ASST 132 128 . . 142 145 + TspEI AATT 141 145 . . 159 163 + Hpy188I TCNGA 161 160 . . 166 169 + SetI ASST 169 165 . . 166 172 + SexAI ACCWGGT 166 171 . . 167 171 + BssKI CCNGG 166 171 . . 167 171 + BseBI CCWGG 168 169 . . 167 171 + ScrFI CCNGG 168 169 . . 167 171 + EcoRII CCWGG 166 171 . . 175 180 + Cac8I GCNNGC 177 177 . . 194 197 + TspEI AATT 193 197 . . 198 201 + CviJI RGCY 199 199 . . 205 208 + AciI CCGC 205 207 . . 207 212 + BtsI GCAGTG 214 212 . . 208 214 + TspRI CASTGNN 214 205 . . 210 215 + BsgI GTGCAG 231 229 . . 211 214 + CviRI* TGCA 212 212 . . 214 219 + XhoII RGATCY 214 218 . . 214 219 + BglII AGATCT 214 218 . . 215 218 + DpnI GATC 216 216 . . 215 218 + BstKTI GATC 217 215 . . 215 218 + MboI GATC 214 218 . . 217 221 + Hpy188I TCNGA 219 218 . . 235 239 + FauI CCCGC 243 245 . . 236 239 + AciI CCGC 236 238 . . 249 254 + AclI AACGTT 250 252 . . 250 253 + TaiI ACGT 253 249 . . 250 253 + SetI ASST 253 249 . . 250 253 + MaeII ACGT 250 252 . . 254 257 + CviRI* TGCA 255 255 . . 258 262 + Tsp4CI* ACNGT 260 259 . . 271 275 - TspDTI ATGAA 261 259 . . 284 287 + TspEI AATT 283 287 . . 286 289 + MseI TTAA 286 288 . . 290 294 + BslFI GGGAC 304 308 . . 290 295 + NlaIV GGNNCC 292 292 . . 291 295 + BmgT120I GGNCC 292 293 . . 291 295 + AvaII GGWCC 291 294 . . 291 295 + AsuI* GGNCC 291 294 . . 302 306 + TspDTI ATGAA 317 315 . . 304 308 + TfiI GAWTC 304 307 . . 304 308 + HinfI GANTC 304 307 . . 310 315 + AhaIII* TTTAAA 312 312 . . 311 314 + MseI TTAA 311 313 . . 322 325 + MseI TTAA 322 324 . . 335 340 + StyI CCWWGG 335 339 . . 335 340 + SecI* CCNNGG 335 339 . . 340 343 - MnlI CCTC 333 332 . . 395 398 + MseI TTAA 395 397 . . 399 412 + AjuI GAANNNNNNNTTGG 391 386 423 418 404 407 + Hin6I GCGC 404 406 . . 404 407 + GlaI GCGC 405 405 . . 404 407 + HhaI GCGC 406 404 . . 412 417 + BtsI GCAGTG 419 417 . . 413 419 + TspRI CASTGNN 419 410 . . 416 421 + CfrI YGGCCR 416 420 . . 417 420 + CviJI RGCY 418 418 . . 417 420 + HaeIII GGCC 418 418 . . 420 424 + Hpy99I CGWCG 424 419 . . 424 428 + HinfI GANTC 424 427 . . 427 431 + Hpy188I TCNGA 429 428 . . 424 428 - PleI GAGTC 418 419 . . 424 428 - MlyI GAGTC 418 418 . . 429 433 + FokI GGATG 442 446 . . 429 433 + BseGI GGATG 435 433 . . 433 437 - BccI CCATC 427 428 . . 442 447 + AflIII ACRYGT 442 446 . . 444 447 + TaiI ACGT 447 443 . . 444 447 + SetI ASST 447 443 . . 444 447 + MaeII ACGT 444 446 . . 454 457 + TspEI AATT 453 457 . . 467 470 + TspEI AATT 466 470 . . 487 490 - MnlI CCTC 480 479 . . 491 495 + TspGWI ACGGA 506 504 . . 503 507 - BslFI GGGAC 488 492 . . 508 514 + TspRI CASTGNN 514 505 . . 508 517 + MslI CAYNNNNRTG 512 512 . . 511 514 + CviRI* TGCA 512 512 . . 512 517 + BsrDI GCAATG 519 517 . . 508 513 - BtsI GCAGTG 507 505 . . 518 528 + BsiYI* CCNNNNNNNGG 524 521 . . 525 529 + TspGWI ACGGA 540 538 . . 529 532 + TspEI AATT 528 532 . . 537 540 + SetI ASST 540 536 . . 535 540 - BspMI ACCTGC 526 530 . . 535 541 - AarI CACCTGC 526 530 . . 543 548 + Hpy178III* TCNNGA 544 546 . . 543 548 + NruI TCGCGA 545 545 . . 544 547 + FnuDII* CGCG 545 545 . . 546 550 + Hpy99I CGWCG 550 545 . . 550 555 + ApoI RAATTY 550 554 . . 551 554 + TspEI AATT 550 554 . . 557 561 + TspDTI ATGAA 572 570 . . 559 563 + MboII GAAGA 571 570 . . 562 566 + MboII GAAGA 574 573 . . 569 573 + Tsp4CI* ACNGT 571 570 . . 570 576 + TspRI CASTGNN 576 567 . . 574 578 - Hin4II* CCTTC 568 567 . . 585 589 + FokI GGATG 598 602 . . 585 589 + BseGI GGATG 591 589 . . 587 591 + TspDTI ATGAA 602 600 . . 582 587 - MmeI TCCRAC 563 561 . . 593 599 + PfoI TCCNGGA 593 598 . . 594 598 + BssKI CCNGG 593 598 . . 594 597 + HpaII CCGG 594 596 . . 594 598 + ScrFI CCNGG 595 596 . . 594 598 + CauII* CCSGG 595 596 . . 602 607 + NspI RCATGY 606 602 . . 602 607 + Cac8I GCNNGC 604 604 . . 602 607 + SphI GCATGC 606 602 . . 603 606 + FatI CATG 602 606 . . 603 606 + NlaIII CATG 606 602 . . 603 606 + CviAII CATG 603 605 . . 621 625 + TfiI GAWTC 621 624 . . 621 625 + HinfI GANTC 621 624 . . 623 628 + ClaI ATCGAT 624 626 . . 624 627 + TaqI TCGA 624 626 . . 633 638 + Hpy166II GTNNAC 635 635 . . 633 638 + AccI GTMKAC 634 636 . . 631 635 - HgaI GACGC 620 625 . . 642 646 - TspGWI ACGGA 632 630 . . 670 674 + Hpy188I TCNGA 672 671 . . 671 676 + Eco57MI CTGRAG 692 690 . . 671 676 + Eco57I CTGAAG 692 690 . . 673 677 + MboII GAAGA 685 684 . . 673 678 + BbvII* GAAGAC 680 684 . . 686 691 + BtsI GCAGTG 693 691 . . 687 693 + TspRI CASTGNN 693 684 . . 691 695 + MboII GAAGA 703 702 . . 694 699 + BseRI GAGGAG 709 707 . . 691 696 - Ksp632I* CTCTTC 686 689 . . 696 701 + NlaIV GGNNCC 698 698 . . 694 697 - MnlI CCTC 687 686 . . 697 702 + SduI GDGCHC 701 697 . . 697 702 + HgiJII* GRGCYC 701 697 . . 698 701 + CviJI RGCY 699 699 . . 702 705 + FatI CATG 701 705 . . 702 705 + NlaIII CATG 705 701 . . 702 705 + CviAII CATG 702 704 . . 707 717 - Hin4I GAYNNNNNVTC 730 725 698 693 718 732 + XcmI CCANNNNNNNNNTGG 725 724 . . 721 724 + MaeI CTAG 721 723 . . 724 727 + CviJI RGCY 725 725 . . 724 727 + HaeIII GGCC 725 725 . . 725 729 - BceAI ACGGC 710 712 . . 731 735 + BmgT120I GGNCC 732 733 . . 731 735 + AsuI* GGNCC 731 734 . . 732 735 + CviJI RGCY 733 733 . . 732 735 + HaeIII GGCC 733 733 . . 734 738 + BssKI CCNGG 733 738 . . 734 738 + BseBI CCWGG 735 736 . . 734 738 + ScrFI CCNGG 735 736 . . 734 738 + EcoRII CCWGG 733 738 . . 736 739 + SetI ASST 739 735 . . 738 742 + Tsp45I GTSAC 737 742 . . 738 742 + MaeIII GTNAC 737 742 . . 757 767 + MwoI GCNNNNNNNGC 763 760 . . 762 766 + FokI GGATG 775 779 . . 762 766 + BseGI GGATG 768 766 . . 763 767 - SfaNI GCATC 753 757 . . 771 775 + FokI GGATG 784 788 . . 771 775 + BseGI GGATG 777 775 . . 776 780 + TspDTI ATGAA 791 789 . . 778 782 + MboII GAAGA 790 789 . . 778 783 + BbvII* GAAGAC 785 789 . . 783 787 + Hpy99I CGWCG 787 782 . . 787 791 + MboII GAAGA 799 798 . . 790 794 + MboII GAAGA 802 801 . . 795 799 + FokI GGATG 808 812 . . 790 795 - Ksp632I* CTCTTC 785 788 . . 795 799 + BseGI GGATG 801 799 . . 793 796 - MnlI CCTC 786 785 . . 799 802 - MnlI CCTC 792 791 . . 802 806 + MboII GAAGA 814 813 . . 805 809 + MboII GAAGA 817 816 . . 808 812 + MboII GAAGA 820 819 . . 811 816 + BseRI GAGGAG 826 824 . . 808 813 - Ksp632I* CTCTTC 803 806 . . 811 814 - MnlI CCTC 804 803 . . 820 824 + TfiI GAWTC 820 823 . . 820 824 + HinfI GANTC 820 823 . . 816 820 - BccI CCATC 810 811 . . 823 827 + Hpy188I TCNGA 825 824 . . 846 850 + FokI GGATG 859 863 . . 846 850 + BseGI GGATG 852 850 . . 844 847 - MnlI CCTC 837 836 . . 851 855 + TspDTI ATGAA 866 864 . . 853 857 + MboII GAAGA 865 864 . . 862 866 + MboII GAAGA 874 873 . . 866 870 + TspDTI ATGAA 881 879 . . 868 872 + MboII GAAGA 880 879 . . 871 875 - BccI CCATC 865 866 . . 882 885 - MnlI CCTC 875 874 . . 885 890 + ApoI RAATTY 885 889 . . 886 889 + TspEI AATT 885 889 . . 900 903 + CviRI* TGCA 901 901 . . 904 909 + SmlI CTYRAG 904 908 . . 899 904 - BsgI GTGCAG 884 882 . . 905 910 + Hpy178III* TCNNGA 906 908 . . 904 909 - Bce83I* CTTGAG 889 887 . . 915 920 + BspLU11I* ACATGT 915 919 . . 915 920 + NspI RCATGY 919 915 . . 915 920 + AflIII ACRYGT 915 919 . . 916 919 + FatI CATG 915 919 . . 916 919 + NlaIII CATG 919 915 . . 916 919 + CviAII CATG 916 918 . . 921 924 + TspEI AATT 920 924 . . 937 940 - MnlI CCTC 930 929 . . 940 944 + MboII GAAGA 952 951 . . 943 948 + BseRI GAGGAG 958 956 . . 940 945 - Ksp632I* CTCTTC 935 938 . . 943 946 - MnlI CCTC 936 935 . . 951 955 + BceAI ACGGC 967 969 . . 957 962 + NspI RCATGY 961 957 . . 958 961 + FatI CATG 957 961 . . 958 961 + NlaIII CATG 961 957 . . 958 961 + CviAII CATG 958 960 . . 962 967 + SfeI* CTRYAG 962 966 . . 967 971 + MboII GAAGA 979 978 . . 967 972 - Ksp632I* CTCTTC 962 965 . . 970 973 - MnlI CCTC 963 962 . . 973 977 + MboII GAAGA 985 984 . . 976 979 + DpnI GATC 977 977 . . 976 979 + BstKTI GATC 978 976 . . 976 979 + MboI GATC 975 979 . . 978 983 + Hpy178III* TCNNGA 979 981 . . 978 983 + NruI TCGCGA 980 980 . . 979 982 + FnuDII* CGCG 980 980 . . 986 990 + Tsp45I GTSAC 985 990 . . 986 990 + MaeIII GTNAC 985 990 . . 1000 1009 + MslI CAYNNNNRTG 1004 1004 . . 1009 1013 - BccI CCATC 1003 1004 . . 1024 1028 + HphI GGTGA 1036 1035 . . 1030 1033 + CviJI RGCY 1031 1031 . . 1032 1042 + EcoNI CCTNNNNNAGG 1036 1037 . . 1032 1042 + BsiYI* CCNNNNNNNGG 1038 1035 . . 1035 1046 + BdaI TGANNNNNNTCA 1024 1022 1058 1056 1036 1045 + BsaBI GATNNNNATC 1040 1040 . . 1041 1045 + BinI* GGATC 1049 1050 . . 1039 1042 - MnlI CCTC 1032 1031 . . 1042 1045 + DpnI GATC 1043 1043 . . 1042 1045 + BstKTI GATC 1044 1042 . . 1042 1045 + MboI GATC 1041 1045 . . 1044 1048 + Hpy188I TCNGA 1046 1045 . . 1035 1046 - BdaI TGANNNNNNTCA 1058 1056 1024 1022 1047 1051 - Hin4II* CCTTC 1041 1040 . . 1052 1055 + SetI ASST 1055 1051 . . 1051 1054 - MnlI CCTC 1044 1043 . . 1054 1058 + MaeIII GTNAC 1053 1058 . . 1059 1063 + TspGWI ACGGA 1074 1072 . . 1061 1066 - BciVI GTATCC 1055 1054 . . 1069 1072 + TaqI TCGA 1069 1071 . . 1075 1079 + Hpy188I TCNGA 1077 1076 . . 1078 1082 + TfiI GAWTC 1078 1081 . . 1078 1082 + HinfI GANTC 1078 1081 . . 1081 1085 + Hpy188I TCNGA 1083 1082 . . 1084 1088 + MboII GAAGA 1096 1095 . . 1087 1090 + DpnI GATC 1088 1088 . . 1087 1090 + BstKTI GATC 1089 1087 . . 1087 1090 + MboI GATC 1086 1090 . . 1091 1096 + Hpy166II GTNNAC 1093 1093 . . 1106 1109 + HpaII CCGG 1106 1108 . . 1108 1111 + CviJI RGCY 1109 1109 . . 1108 1113 + NlaIV GGNNCC 1110 1110 . . 1110 1115 - Eco57MI CTGRAG 1095 1093 . . 1115 1120 + BtgZI GCGATG 1130 1134 . . 1110 1115 - GsuI CTGGAG 1095 1093 . . 1144 1148 + TspGWI ACGGA 1159 1157 . . 1147 1151 + MboII GAAGA 1159 1158 . . 1151 1154 + TspEI AATT 1150 1154 . . 1179 1188 + XmnI GAANNNNTTC 1183 1183 . . 1187 1191 - TspGWI ACGGA 1177 1175 . . 1198 1201 + MaeI CTAG 1198 1200 . . 1205 1209 + Tsp4CI* ACNGT 1207 1206 . . 1206 1212 + TspRI CASTGNN 1212 1203 . . 1202 1206 - BsmAI GTCTC 1196 1200 . . 1208 1212 + Tsp45I GTSAC 1207 1212 . . 1208 1212 + MaeIII GTNAC 1207 1212 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 526 695 # # AccI: # [GTMKAC] # 587 634 # # AciI: # [CCGC] # 31 205 985 # # AclI: # [AACGTT] # 250 971 # # AcyI: # [GRCGYC] # 53 1168 # # AflIII: # [ACRYGT] # 306 442 473 # # AhaIII*: # [TTTAAA] # 312 909 # # AjuI: # [GAANNNNNNNTTGG] # 32 391 798 # # ApoI: # [RAATTY] # 335 336 550 # # AsuI*: # [GGNCC] # 291 440 490 # # AvaII: # [GGWCC] # 291 930 # # BbvII*: # [GAAGAC] # 105 436 680 # # BccI: # [CCATC] # 34 55 66 138 218 327 # 383 # # Bce83I*: # [CTTGAG] # 332 889 # # BceAI: # [ACGGC] # 254 257 710 # # BciVI: # [GTATCC] # 166 1055 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 163 1024 # # BglII: # [AGATCT] # 214 1007 # # BinI*: # [GGATC] # 172 1049 # # BmgT120I: # [GGNCC] # 292 440 489 # # BsaBI: # [GATNNNNATC] # 181 1040 # # BseBI: # [CCWGG] # 168 486 567 # # BseGI: # [GGATG] # 9 24 51 156 177 369 # 435 # # BseRI: # [GAGGAG] # 117 132 263 709 # # BsgI: # [GTGCAG] # 231 337 653 # # BsiYI*: # [CCNNNNNNNGG] # 183 514 524 # # BslFI: # [GGGAC] # 184 304 733 # # BsmAI: # [GTCTC] # 25 1196 # # BspLU11I*: # [ACATGT] # 306 915 # # BspMI: # [ACCTGC] # 526 695 # # BsrDI: # [GCAATG] # 519 702 # # BssKI: # [CCNGG] # 140 166 427 488 # # BstKTI: # [GATC] # 45 66 132 217 761 # # BstXI: # [CCANNNNNNTGG] # 40 1181 # # BtgZI: # [GCGATG] # 91 1130 # # BtsI: # [GCAGTG] # 88 186 205 214 528 # # Cac8I: # [GCNNGC] # 177 427 617 # # CauII*: # [CCSGG] # 595 626 # # CfrI: # [YGGCCR] # 416 805 # # ClaI: # [ATCGAT] # 597 624 # # Csp6I: # [GTAC] # 115 1106 # # CviAII: # [CATG] # 42 99 214 263 603 # # CviJI: # [RGCY] # 8 26 78 112 199 219 # 281 298 # # CviRI*: # [TGCA] # 43 212 257 320 389 # # DpnI: # [GATC] # 45 66 133 216 761 # # DraIII: # [CACNNNGTG] # 133 1088 # # Eco57I: # [CTGAAG] # 529 692 # # Eco57MI: # [CTGRAG] # 126 403 692 # # EcoNI: # [CCTNNNNNAGG] # 185 1036 # # EcoRII: # [CCWGG] # 166 488 567 # # FatI: # [CATG] # 42 99 214 264 602 # # FauI: # [CCCGC] # 243 978 # # FnuDII*: # [CGCG] # 241 435 545 # # FokI: # [GGATG] # 9 24 51 156 177 362 # 442 # # GlaI: # [GCGC] # 405 816 # # GsuI: # [CTGGAG] # 126 1095 # # HaeIII: # [GGCC] # 8 307 418 488 # # HgaI: # [GACGC] # 61 559 601 # # HgiCI*: # [GGYRCC] # 43 1178 # # HgiJII*: # [GRGCYC] # 520 701 # # HhaI: # [GCGC] # 406 815 # # Hin4I: # [GAYNNNNNVTC] # 32 491 698 # # Hin4II*: # [CCTTC] # 180 473 568 # # Hin6I: # [GCGC] # 404 817 # # HinfI: # [GANTC] # 76 120 143 197 199 228 # 258 # # HpaII: # [CCGG] # 115 512 594 # # HphI: # [GGTGA] # 22 185 1014 # # Hpy166II: # [GTNNAC] # 25 128 458 610 # # Hpy178III*: # [TCNNGA] # 73 242 362 544 # # Hpy188I: # [TCNGA] # 6 31 58 138 153 161 # 210 221 243 # # Hpy99I: # [CGWCG] # 10 126 237 414 434 # # Ksp632I*: # [CTCTTC] # 18 27 99 132 259 686 # # MaeI: # [CTAG] # 23 477 721 # # MaeII: # [ACGT] # 194 250 777 # # MaeIII: # [GTNAC] # 14 68 154 248 737 # # MboI: # [GATC] # 45 66 135 214 761 # # MboII: # [GAAGA] # 3 3 3 3 6 6 # 9 9 12 18 27 45 # 62 63 72 87 111 111 # 571 # # MlyI: # [GAGTC] # 418 803 # # MmeI: # [TCCRAC] # 563 658 # # MnlI: # [CCTC] # 6 6 12 12 27 33 # 38 55 69 99 147 177 # 207 333 # # MseI: # [TTAA] # 11 25 73 286 826 # # MslI: # [CAYNNNNRTG] # 217 492 512 # # MwoI: # [GCNNNNNNNGC] # 95 458 668 # # NlaIII: # [CATG] # 42 99 214 260 606 # # NlaIV: # [GGNNCC] # 45 111 247 406 412 # # NruI: # [TCGCGA] # 241 435 545 # # NspI: # [RCATGY] # 42 260 313 606 # # PfoI: # [TCCNGGA] # 593 628 # # PleI: # [GAGTC] # 418 803 # # PsiI: # [TTATAA] # 65 1156 # # RsaI: # [GTAC] # 116 1105 # # ScrFI: # [CCNGG] # 140 168 427 486 # # SduI: # [GDGCHC] # 520 701 # # SecI*: # [CCNNGG] # 47 288 886 # # SetI: # [ASST] # 37 84 93 132 166 194 # 199 316 # # SexAI: # [ACCWGGT] # 166 1055 # # SfaNI: # [GCATC] # 468 753 # # SfeI*: # [CTRYAG] # 259 962 # # SmlI: # [CTYRAG] # 317 904 # # SphI: # [GCATGC] # 606 615 # # StyI: # [CCWWGG] # 47 288 886 # # TaiI: # [ACGT] # 194 253 774 # # TaqI: # [TCGA] # 152 445 624 # # TfiI: # [GAWTC] # 76 143 199 228 258 317 # # Tsp45I: # [GTSAC] # 14 222 248 737 # # Tsp4CI*: # [ACNGT] # 14 22 238 311 636 # # TspDTI: # [ATGAA] # 15 30 56 75 189 255 # 261 340 # # TspEI: # [AATT] # 10 13 22 35 52 62 # 71 90 131 170 230 335 # # TspGWI: # [ACGGA] # 18 34 44 85 92 442 # 506 # # TspRI: # [CASTGNN] # 9 62 95 117 205 214 # 519 # # XcmI: # [CCANNNNNNNNNTGG] # 496 725 # # XhoII: # [RGATCY] # 214 1007 # # XmnI: # [GAANNNNTTC] # 38 1183 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1221 # Reported_sequences: 1 # Reported_hitcount: 299 #---------------------------------------