Restriction Fragments for ADH2/YMR303C



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The currently selected gene/sequence is :

ADH2/YMR303C on chromosome XIII from coordinates 874337 to 873291.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.24125.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 14 + Hpy178III* TCNNGA 10 12 . . 24 27 + CviJI RGCY 25 25 . . 32 36 - MboII GAAGA 24 23 . . 40 44 + TfiI GAWTC 40 43 . . 40 44 + HinfI GANTC 40 43 . . 43 48 + MmeI TCCRAC 68 66 . . 44 58 + XcmI CCANNNNNNNNNTGG 51 50 . . 47 51 + BceAI ACGGC 63 65 . . 54 59 - MmeI TCCRAC 35 33 . . 67 72 + EcoRV GATATC 69 69 . . 73 77 - BsrI ACTGG 73 71 . . 72 77 - BfiI ACTGGG 67 66 . . 83 86 + CviJI RGCY 84 84 . . 89 92 + CviJI RGCY 90 90 . . 98 101 + TspEI AATT 97 101 . . 103 106 + MseI TTAA 103 105 . . 110 113 + TaiI ACGT 113 109 . . 110 113 + SetI ASST 113 109 . . 110 113 + MaeII ACGT 110 112 . . 116 121 + TatI WGTACW 116 120 . . 116 121 + ScaI AGTACT 118 118 . . 117 120 + Csp6I GTAC 117 119 . . 117 120 + RsaI GTAC 118 118 . . 143 146 + CviRI* TGCA 144 144 . . 144 154 + MwoI GCNNNNNNNGC 150 147 . . 144 149 + Cac8I GCNNGC 146 146 . . 154 157 + FatI CATG 153 157 . . 154 157 + NlaIII CATG 157 153 . . 154 157 + CviAII CATG 154 156 . . 157 161 + HphI GGTGA 169 168 . . 158 162 + Tsp45I GTSAC 157 162 . . 158 162 + MaeIII GTNAC 157 162 . . 161 165 + BsrI ACTGG 166 164 . . 163 168 + BalI TGGCCA 165 165 . . 163 168 + CfrI YGGCCR 163 167 . . 164 167 + CviJI RGCY 165 165 . . 164 167 + HaeIII GGCC 165 165 . . 167 172 - BsrDI GCAATG 166 164 . . 176 180 + DdeI CTNAG 176 179 . . 180 184 + MaeIII GTNAC 179 184 . . 184 194 + BsiYI* CCNNNNNNNGG 190 187 . . 184 194 + PflMI CCANNNNNTGG 190 187 . . 197 201 + Tsp45I GTSAC 196 201 . . 197 201 + MaeIII GTNAC 196 201 . . 198 203 + Hpy178III* TCNNGA 199 201 . . 199 208 + MslI CAYNNNNRTG 203 203 . . 199 208 + OliI CACNNNNGTG 203 203 . . 204 207 + SetI ASST 207 203 . . 205 210 + NlaIV GGNNCC 207 207 . . 205 210 + HgiCI* GGYRCC 205 209 . . 202 206 - Hin4II* CCTTC 196 195 . . 208 213 + Cfr10I RCCGGY 208 212 . . 209 212 + HpaII CCGG 209 211 . . 225 228 + FatI CATG 224 228 . . 225 228 + NlaIII CATG 228 224 . . 225 228 + CviAII CATG 225 227 . . 229 233 + HphI GGTGA 241 240 . . 227 232 - TaqII CACCCA 217 215 . . 235 240 + AclI AACGTT 236 238 . . 236 239 + TaiI ACGT 239 235 . . 236 239 + SetI ASST 239 235 . . 236 239 + MaeII ACGT 236 238 . . 239 242 + MseI TTAA 239 241 . . 244 247 + CviJI RGCY 245 245 . . 249 253 + MboII GAAGA 261 260 . . 252 255 + DpnI GATC 253 253 . . 252 255 + BstKTI GATC 254 252 . . 252 255 + MboI GATC 251 255 . . 256 260 + HphI GGTGA 268 267 . . 257 261 + Tsp45I GTSAC 256 261 . . 257 261 + MaeIII GTNAC 256 261 . . 248 259 - TstI CACNNNNNNTCC 272 267 240 235 263 273 + BaeI ACNNNNGTAYC 252 247 285 280 265 270 + Cfr10I RCCGGY 265 269 . . 266 269 + HpaII CCGG 266 268 . . 284 288 + Tsp4CI* ACNGT 286 285 . . 297 300 + CviJI RGCY 298 298 . . 297 300 + HaeIII GGCC 298 298 . . 308 312 + Tsp4CI* ACNGT 310 309 . . 314 317 + TspEI AATT 313 317 . . 320 324 + MaeIII GTNAC 319 324 . . 325 329 + TfiI GAWTC 325 328 . . 325 329 + HinfI GANTC 325 328 . . 328 333 + MmeI TCCRAC 353 351 . . 332 336 + Tsp4CI* ACNGT 334 333 . . 337 340 + MnlI CCTC 347 346 . . 356 360 + MaeIII GTNAC 355 360 . . 354 359 - TsoI TARCCA 344 342 . . 360 365 + TaqII CACCCA 376 374 . . 366 370 + Hpy99I CGWCG 370 365 . . 368 372 + Tsp4CI* ACNGT 370 369 . . 392 397 + NspBII* CMGCKG 394 394 . . 392 395 + AciI CCGC 392 394 . . 396 407 + BdaI TGANNNNNNTCA 385 383 419 417 397 401 + HgaI GACGC 406 411 . . 400 410 + MwoI GCNNNNNNNGC 406 403 . . 396 407 - BdaI TGANNNNNNTCA 419 417 385 383 408 411 + CviJI RGCY 409 409 . . 409 413 + TauI GCSGC 412 409 . . 409 413 + BlsI GCNGC 411 410 . . 409 413 + BisI GCNGC 410 411 . . 410 413 + AciI CCGC 410 412 . . 410 415 + BsrBI CCGCTC 412 412 . . 421 424 + MnlI CCTC 431 430 . . 422 427 + SmlI CTYRAG 422 426 . . 426 429 + SetI ASST 429 425 . . 422 427 - Bce83I* CTTGAG 407 405 . . 428 431 + Csp6I GTAC 428 430 . . 428 431 + RsaI GTAC 429 429 . . 438 441 + CviJI RGCY 439 439 . . 439 449 + MwoI GCNNNNNNNGC 445 442 . . 440 445 + Eco57MI CTGRAG 461 459 . . 440 445 + Eco57I CTGAAG 461 459 . . 447 450 + FnuDII* CGCG 448 448 . . 448 451 + Hin6I GCGC 448 450 . . 448 451 + GlaI GCGC 449 449 . . 448 451 + HhaI GCGC 450 448 . . 472 476 + Tsp4CI* ACNGT 474 473 . . 470 474 - HphI GGTGA 462 461 . . 475 480 + Hpy166II GTNNAC 477 477 . . 475 480 + BssNAI GTATAC 477 477 . . 475 480 + AccI GTMKAC 476 478 . . 483 486 + CviJI RGCY 484 484 . . 501 506 + SmlI CTYRAG 501 505 . . 501 506 + Bce83I* CTTGAG 522 520 . . 508 513 + Cac8I GCNNGC 510 510 . . 511 514 + CviJI RGCY 512 512 . . 511 514 + HaeIII GGCC 512 512 . . 513 523 + BsiYI* CCNNNNNNNGG 519 516 . . 514 520 + TspRI CASTGNN 520 511 . . 515 519 + BsrI ACTGG 520 518 . . 515 520 + BfiI ACTGGG 525 524 . . 520 524 + TauI GCSGC 523 520 . . 520 524 + BlsI GCNGC 522 521 . . 520 524 + BisI GCNGC 521 522 . . 521 526 + CfrI YGGCCR 521 525 . . 522 525 + CviJI RGCY 523 523 . . 522 525 + HaeIII GGCC 523 523 . . 520 523 - AciI CCGC 520 522 . . 535 539 + BlsI GCNGC 537 536 . . 535 539 + BisI GCNGC 536 537 . . 535 539 + TseI GCWGC 535 538 . . 535 539 - BbvI GCAGC 522 526 . . 536 541 - EcoP15I CAGCAG 508 510 . . 547 550 + MaeI CTAG 547 549 . . 549 552 + SetI ASST 552 548 . . 558 561 + CviJI RGCY 559 559 . . 572 576 + DdeI CTNAG 572 575 . . 577 582 + BtgZI GCGATG 592 596 . . 571 577 - Bpu10I CCTNAGC 572 575 . . 579 583 - BccI CCATC 573 574 . . 584 588 + MaeIII GTNAC 583 588 . . 590 594 + PleI GAGTC 598 599 . . 590 594 + MlyI GAGTC 599 599 . . 590 594 + HinfI GANTC 590 593 . . 594 598 + DdeI CTNAG 594 597 . . 597 600 + SetI ASST 600 596 . . 604 608 - BccI CCATC 598 599 . . 610 614 + BmgT120I GGNCC 611 612 . . 610 614 + AvaII GGWCC 610 613 . . 610 614 + AsuI* GGNCC 610 613 . . 612 618 + PfoI TCCNGGA 612 617 . . 613 617 + BssKI CCNGG 612 617 . . 613 617 + BseBI CCWGG 614 615 . . 613 617 + ScrFI CCNGG 614 615 . . 613 617 + EcoRII CCWGG 612 617 . . 622 626 + MboII GAAGA 634 633 . . 626 629 + TspEI AATT 625 629 . . 630 635 + Hpy166II GTNNAC 632 632 . . 634 637 + SetI ASST 637 633 . . 635 638 + MnlI CCTC 645 644 . . 646 650 + HphI GGTGA 658 657 . . 655 659 - TspDTI ATGAA 645 643 . . 659 662 + TaqI TCGA 659 661 . . 665 669 - HphI GGTGA 657 656 . . 675 679 - Hin4II* CCTTC 669 668 . . 689 692 + Hin6I GCGC 689 691 . . 689 692 + GlaI GCGC 690 690 . . 689 692 + HhaI GCGC 691 689 . . 698 701 + MseI TTAA 698 700 . . 702 705 + CviJI RGCY 703 703 . . 710 714 + BceAI ACGGC 726 728 . . 715 720 + NlaIV GGNNCC 717 717 . . 715 720 + HgiCI* GGYRCC 715 719 . . 716 721 + SduI GDGCHC 720 716 . . 716 721 + BseSI GKGCMC 720 716 . . 713 716 - AciI CCGC 713 715 . . 720 725 + DsaI* CCRYGG 720 724 . . 720 725 + SecI* CCNNGG 720 724 . . 722 726 + Tsp4CI* ACNGT 724 723 . . 739 743 - TspGWI ACGGA 729 727 . . 745 749 + Hpy188I TCNGA 747 746 . . 750 753 + CviJI RGCY 751 751 . . 751 755 + TauI GCSGC 754 751 . . 751 755 + BlsI GCNGC 753 752 . . 751 755 + BisI GCNGC 752 753 . . 752 755 + AciI CCGC 752 754 . . 754 764 + MwoI GCNNNNNNNGC 760 757 . . 758 761 + TaqI TCGA 758 760 . . 761 766 + HindIII AAGCTT 761 765 . . 762 765 + SetI ASST 765 761 . . 762 765 + CviJI RGCY 763 763 . . 762 765 + AluI AGCT 763 763 . . 771 779 + AlwNI CAGNNNCTG 776 773 . . 776 780 + Tsp4CI* ACNGT 778 777 . . 777 782 + SfeI* CTRYAG 777 781 . . 788 792 + Tsp4CI* ACNGT 790 789 . . 791 794 + Csp6I GTAC 791 793 . . 791 794 + RsaI GTAC 792 792 . . 793 797 + Tsp4CI* ACNGT 795 794 . . 790 800 - BaeI ACNNNNGTAYC 815 810 782 777 813 818 + Cac8I GCNNGC 815 815 . . 816 819 + CviJI RGCY 817 817 . . 817 822 + Cfr10I RCCGGY 817 821 . . 818 821 + HpaII CCGG 818 820 . . 822 825 + CviRI* TGCA 823 823 . . 828 833 + SduI GDGCHC 832 828 . . 828 833 + HgiAI* GWGCWC 832 828 . . 833 836 + MnlI CCTC 843 842 . . 835 839 + Hpy188I TCNGA 837 836 . . 831 836 - BseRI GAGGAG 822 820 . . 842 846 - MboII GAAGA 834 833 . . 841 846 - BbvII* GAAGAC 834 838 . . 851 854 + TaiI ACGT 854 850 . . 851 854 + SetI ASST 854 850 . . 851 854 + MaeII ACGT 851 853 . . 877 880 + CviJI RGCY 878 878 . . 883 888 + BsaAI YACGTR 885 885 . . 884 887 + TaiI ACGT 887 883 . . 884 887 + SetI ASST 887 883 . . 884 887 + MaeII ACGT 884 886 . . 897 900 + SetI ASST 900 896 . . 897 900 + CviJI RGCY 898 898 . . 897 900 + AluI AGCT 898 898 . . 912 915 + CviJI RGCY 913 913 . . 915 919 + DdeI CTNAG 915 918 . . 933 936 + SetI ASST 936 932 . . 932 935 - MnlI CCTC 925 924 . . 937 940 + MaeI CTAG 937 939 . . 932 942 - Hin4I GAYNNNNNVTC 955 950 923 918 945 949 + BsmAI GTCTC 950 954 . . 956 959 + SetI ASST 959 955 . . 964 967 + CviJI RGCY 965 965 . . 974 979 + Hpy166II GTNNAC 976 976 . . 971 975 - BsrI ACTGG 971 969 . . 983 988 + ApoI RAATTY 983 987 . . 984 987 + TspEI AATT 983 987 . . 996 1000 - BccI CCATC 990 991 . . 1005 1009 + BmgT120I GGNCC 1006 1007 . . 1005 1009 + AsuI* GGNCC 1005 1008 . . 1002 1006 - Hin4II* CCTTC 996 995 . . 1006 1009 + CviJI RGCY 1007 1007 . . 1006 1009 + HaeIII GGCC 1007 1007 . . 1008 1019 + BstXI CCANNNNNNTGG 1015 1011 . . 1011 1014 + TspEI AATT 1010 1014 . . 1025 1028 + TaiI ACGT 1028 1024 . . 1025 1028 + SetI ASST 1028 1024 . . 1025 1028 + MaeII ACGT 1025 1027 . . 1030 1035 + Hpy166II GTNNAC 1032 1032 . . 1030 1035 + HindII GTYRAC 1032 1032 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 476 571 # # AciI: # [CCGC] # 18 39 110 193 295 392 # # AclI: # [AACGTT] # 236 811 # # AluI: # [AGCT] # 135 149 763 # # AlwNI: # [CAGNNNCTG] # 271 776 # # ApoI: # [RAATTY] # 64 983 # # AsuI*: # [GGNCC] # 42 395 610 # # AvaII: # [GGWCC] # 437 610 # # BaeI: # [ACNNNNGTAYC] # 33 33 232 252 497 # # BalI: # [TGGCCA] # 165 882 # # BbvI: # [GCAGC] # 522 525 # # BbvII*: # [GAAGAC] # 213 834 # # BccI: # [CCATC] # 25 57 392 573 # # Bce83I*: # [CTTGAG] # 115 407 525 # # BceAI: # [ACGGC] # 63 321 663 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 385 628 # # BfiI: # [ACTGGG] # 67 458 522 # # BisI: # [GCNGC] # 15 111 216 295 410 # # BlsI: # [GCNGC] # 15 111 216 294 411 # # BmgT120I: # [GGNCC] # 41 395 611 # # Bpu10I: # [CCTNAGC] # 475 572 # # BsaAI: # [YACGTR] # 162 885 # # BseBI: # [CCWGG] # 433 614 # # BseRI: # [GAGGAG] # 225 822 # # BseSI: # [GKGCMC] # 327 720 # # BsiYI*: # [CCNNNNNNNGG] # 190 329 528 # # BsmAI: # [GTCTC] # 97 950 # # BsrBI: # [CCGCTC] # 412 635 # # BsrDI: # [GCAATG] # 166 881 # # BsrI: # [ACTGG] # 73 76 93 354 451 # # BssKI: # [CCNGG] # 435 612 # # BssNAI: # [GTATAC] # 477 570 # # BstKTI: # [GATC] # 254 793 # # BstXI: # [CCANNNNNNTGG] # 32 1015 # # BtgZI: # [GCGATG] # 455 592 # # Cac8I: # [GCNNGC] # 146 232 305 364 # # Cfr10I: # [RCCGGY] # 57 208 230 552 # # CfrI: # [YGGCCR] # 163 358 526 # # Csp6I: # [GTAC] # 117 256 311 363 # # CviAII: # [CATG] # 71 154 822 # # CviJI: # [RGCY] # 6 11 12 15 20 25 # 28 30 36 40 42 45 # 48 52 53 54 59 61 # 75 80 111 144 # # CviRI*: # [TGCA] # 144 224 679 # # DdeI: # [CTNAG] # 22 132 176 321 396 # # DpnI: # [GATC] # 253 794 # # DsaI*: # [CCRYGG] # 327 720 # # Eco57I: # [CTGAAG] # 461 586 # # Eco57MI: # [CTGRAG] # 461 586 # # EcoP15I: # [CAGCAG] # 508 539 # # EcoRII: # [CCWGG] # 435 612 # # EcoRV: # [GATATC] # 69 978 # # FatI: # [CATG] # 71 153 823 # # FnuDII*: # [CGCG] # 448 599 # # GlaI: # [GCGC] # 241 357 449 # # HaeIII: # [GGCC] # 11 40 133 165 214 484 # # HgaI: # [GACGC] # 406 641 # # HgiAI*: # [GWGCWC] # 215 832 # # HgiCI*: # [GGYRCC] # 205 332 510 # # HhaI: # [GCGC] # 241 356 450 # # Hin4I: # [GAYNNNNNVTC] # 32 92 923 # # Hin4II*: # [CCTTC] # 51 196 327 473 # # Hin6I: # [GCGC] # 241 358 448 # # HindII: # [GTYRAC] # 15 1032 # # HindIII: # [AAGCTT] # 286 761 # # HinfI: # [GANTC] # 40 265 285 457 # # HpaII: # [CCGG] # 57 209 229 552 # # HphI: # [GGTGA] # 1 27 72 169 194 195 # 389 # # Hpy166II: # [GTNNAC] # 15 56 155 344 477 # # Hpy178III*: # [TCNNGA] # 10 189 848 # # Hpy188I: # [TCNGA] # 90 210 747 # # Hpy99I: # [CGWCG] # 370 677 # # MaeI: # [CTAG] # 110 390 547 # # MaeII: # [ACGT] # 22 33 110 126 141 615 # # MaeIII: # [GTNAC] # 17 22 36 60 63 157 # 228 464 # # MboI: # [GATC] # 251 796 # # MboII: # [GAAGA] # 24 200 213 237 373 # # MlyI: # [GAGTC] # 448 599 # # MmeI: # [TCCRAC] # 33 35 285 694 # # MnlI: # [CCTC] # 82 84 122 198 214 347 # # MseI: # [TTAA] # 103 136 349 459 # # MslI: # [CAYNNNNRTG] # 203 844 # # MwoI: # [GCNNNNNNNGC] # 39 150 256 287 315 # # NlaIII: # [CATG] # 71 157 819 # # NlaIV: # [GGNNCC] # 207 330 510 # # NspBII*: # [CMGCKG] # 394 653 # # OliI: # [CACNNNNGTG] # 203 844 # # PflMI: # [CCANNNNNTGG] # 190 857 # # PfoI: # [TCCNGGA] # 435 612 # # PleI: # [GAGTC] # 449 598 # # RsaI: # [GTAC] # 118 255 311 363 # # ScaI: # [AGTACT] # 118 929 # # ScrFI: # [CCNGG] # 433 614 # # SduI: # [GDGCHC] # 112 215 720 # # SecI*: # [CCNNGG] # 327 720 # # SetI: # [ASST] # 13 19 23 32 33 36 # 37 48 69 89 94 113 # 123 128 190 # # SfeI*: # [CTRYAG] # 270 777 # # SmlI: # [CTYRAG] # 79 422 546 # # TaiI: # [ACGT] # 19 33 113 126 141 615 # # TaqI: # [TCGA] # 99 289 659 # # TaqII: # [CACCCA] # 159 217 671 # # TatI: # [WGTACW] # 116 931 # # TauI: # [GCSGC] # 111 231 293 412 # # TfiI: # [GAWTC] # 40 285 722 # # TseI: # [GCWGC] # 512 535 # # TsoI: # [TARCCA] # 344 703 # # Tsp45I: # [GTSAC] # 39 60 157 791 # # Tsp4CI*: # [ACNGT] # 5 12 24 24 36 54 # 104 250 252 286 # # TspDTI: # [ATGAA] # 402 645 # # TspEI: # [AATT] # 27 37 97 216 312 358 # # TspGWI: # [ACGGA] # 318 729 # # TspRI: # [CASTGNN] # 520 527 # # TstI: # [CACNNNNNNTCC] # 32 240 775 # # XcmI: # [CCANNNNNNNNNTGG] # 51 996 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1047 # Reported_sequences: 1 # Reported_hitcount: 260 #---------------------------------------