Restriction Fragments for CYC1/YJR048W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

CYC1/YJR048W on chromosome X from coordinates 526335 to 526664.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.26032.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 12 + EcoRI GAATTC 7 11 . . 7 12 + ApoI RAATTY 7 11 . . 8 11 + TspEI AATT 7 11 . . 2 13 - BdaI TGANNNNNNTCA 25 23 . . 15 18 + CviJI RGCY 16 16 . . 15 18 + HaeIII GGCC 16 16 . . 16 26 + MwoI GCNNNNNNNGC 22 19 . . 16 21 + Cfr10I RCCGGY 16 20 . . 17 20 + HpaII CCGG 17 19 . . 26 30 + DdeI CTNAG 26 29 . . 33 36 + SetI ASST 36 32 . . 47 52 + Hpy178III* TCNNGA 48 50 . . 51 61 + Hin4I GAYNNNNNVTC 42 37 74 69 51 61 + Eam1105I GACNNNNNGTC 56 55 . . 53 56 + MaeI CTAG 53 55 . . 59 64 + Hpy166II GTNNAC 61 61 . . 59 64 + AccI GTMKAC 60 62 . . 51 61 - Hin4I GAYNNNNNVTC 74 69 42 37 69 79 + BsiYI* CCNNNNNNNGG 75 72 . . 69 79 + PflMI CCANNNNNTGG 75 72 . . 70 78 + DraIII CACNNNGTG 75 72 . . 73 77 + Tsp4CI* ACNGT 75 74 . . 74 79 + DsaI* CCRYGG 74 78 . . 74 79 + SecI* CCNNGG 74 78 . . 88 91 + CviJI RGCY 89 89 . . 88 91 + HaeIII GGCC 89 89 . . 88 92 + BmgT120I GGNCC 89 90 . . 88 92 + AsuI* GGNCC 88 91 . . 90 100 + BsiYI* CCNNNNNNNGG 96 93 . . 98 101 + SetI ASST 101 97 . . 103 107 + BmgT120I GGNCC 104 105 . . 103 107 + AvaII GGWCC 103 106 . . 103 107 + AsuI* GGNCC 103 106 . . 113 116 + CviRI* TGCA 114 114 . . 115 118 + FatI CATG 114 118 . . 115 118 + NlaIII CATG 118 114 . . 115 118 + CviAII CATG 115 117 . . 131 142 + DrdI GACNNNNNNGTC 137 135 . . 144 147 + SetI ASST 147 143 . . 144 147 + CviJI RGCY 145 145 . . 144 147 + AluI AGCT 145 145 . . 146 151 + Eco57MI CTGRAG 167 165 . . 146 151 + Eco57I CTGAAG 167 165 . . 148 152 - Hin4II* CCTTC 142 141 . . 159 164 + Hpy166II GTNNAC 161 161 . . 159 162 + Csp6I GTAC 159 161 . . 159 162 + RsaI GTAC 160 160 . . 166 170 - SfaNI GCATC 156 160 . . 176 181 + Hpy178III* TCNNGA 177 179 . . 185 188 + TaiI ACGT 188 184 . . 185 188 + SetI ASST 188 184 . . 185 188 + MaeII ACGT 185 187 . . 185 190 + AflIII ACRYGT 185 189 . . 194 198 + BslFI GGGAC 208 212 . . 206 211 + BspLU11I* ACATGT 206 210 . . 206 211 + NspI RCATGY 210 206 . . 206 211 + AflIII ACRYGT 206 210 . . 207 210 + FatI CATG 206 210 . . 207 210 + NlaIII CATG 210 206 . . 207 210 + CviAII CATG 207 209 . . 211 215 + Hpy188I TCNGA 213 212 . . 215 220 + TatI WGTACW 215 219 . . 215 220 + ScaI AGTACT 217 217 . . 216 219 + Csp6I GTAC 216 218 . . 216 219 + RsaI GTAC 217 217 . . 244 248 + BssKI CCNGG 243 248 . . 244 248 + BseBI CCWGG 245 246 . . 244 248 + ScrFI CCNGG 245 246 . . 244 248 + EcoRII CCWGG 243 248 . . 247 252 + KpnI GGTACC 251 247 . . 247 252 + NlaIV GGNNCC 249 249 . . 247 252 + HgiCI* GGYRCC 247 251 . . 247 252 + Acc65I GGTACC 247 251 . . 248 251 + Csp6I GTAC 248 250 . . 248 251 + RsaI GTAC 249 249 . . 253 263 + FalI AAGNNNNNCTT 244 239 276 271 258 261 + CviJI RGCY 259 259 . . 258 261 + HaeIII GGCC 259 259 . . 255 259 - BccI CCATC 249 250 . . 260 270 + BsiYI* CCNNNNNNNGG 266 263 . . 253 263 - FalI AAGNNNNNCTT 276 271 244 239 273 277 + MboII GAAGA 285 284 . . 276 280 - Hin4II* CCTTC 270 269 . . 298 301 + MseI TTAA 298 300 . . 300 303 + TspEI AATT 299 303 . . 304 307 + SetI ASST 307 303 . . 318 321 + CviJI RGCY 319 319 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 83 247 # # AccI: # [GTMKAC] # 60 270 # # AflIII: # [ACRYGT] # 21 124 185 # # AluI: # [AGCT] # 145 185 # # ApoI: # [RAATTY] # 7 323 # # AsuI*: # [GGNCC] # 15 88 227 # # AvaII: # [GGWCC] # 103 227 # # BccI: # [CCATC] # 81 249 # # BdaI: # [TGANNNNNNTCA] # 25 305 # # BmgT120I: # [GGNCC] # 15 89 226 # # BseBI: # [CCWGG] # 85 245 # # BsiYI*: # [CCNNNNNNNGG] # 21 64 75 170 # # BslFI: # [GGGAC] # 122 208 # # BspLU11I*: # [ACATGT] # 124 206 # # BssKI: # [CCNGG] # 87 243 # # Cfr10I: # [RCCGGY] # 16 314 # # Csp6I: # [GTAC] # 32 57 82 159 # # CviAII: # [CATG] # 92 115 123 # # CviJI: # [RGCY] # 11 16 56 60 73 114 # # CviRI*: # [TGCA] # 114 216 # # DdeI: # [CTNAG] # 26 304 # # DraIII: # [CACNNNGTG] # 75 255 # # DrdI: # [GACNNNNNNGTC] # 137 193 # # DsaI*: # [CCRYGG] # 74 256 # # Eam1105I: # [GACNNNNNGTC] # 56 274 # # Eco57I: # [CTGAAG] # 163 167 # # Eco57MI: # [CTGRAG] # 163 167 # # EcoRI: # [GAATTC] # 7 323 # # EcoRII: # [CCWGG] # 87 243 # # FalI: # [AAGNNNNNCTT] # 0 0 32 54 244 # # FatI: # [CATG] # 92 114 124 # # HaeIII: # [GGCC] # 16 71 73 170 # # HgiCI*: # [GGYRCC] # 83 247 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 42 256 # # Hin4II*: # [CCTTC] # 60 128 142 # # HpaII: # [CCGG] # 17 313 # # Hpy166II: # [GTNNAC] # 61 100 169 # # Hpy178III*: # [TCNNGA] # 48 129 153 # # Hpy188I: # [TCNGA] # 117 213 # # KpnI: # [GGTACC] # 79 251 # # MaeI: # [CTAG] # 53 277 # # MaeII: # [ACGT] # 145 185 # # MboII: # [GAAGA] # 45 285 # # MseI: # [TTAA] # 32 298 # # MwoI: # [GCNNNNNNNGC] # 22 308 # # NlaIII: # [CATG] # 92 118 120 # # NlaIV: # [GGNNCC] # 81 249 # # NspI: # [RCATGY] # 120 210 # # PflMI: # [CCANNNNNTGG] # 75 255 # # RsaI: # [GTAC] # 32 57 81 160 # # ScaI: # [AGTACT] # 113 217 # # ScrFI: # [CCNGG] # 85 245 # # SecI*: # [CCNNGG] # 74 256 # # SetI: # [ASST] # 23 36 41 46 65 119 # # SfaNI: # [GCATC] # 156 174 # # TaiI: # [ACGT] # 142 188 # # TatI: # [WGTACW] # 115 215 # # Tsp4CI*: # [ACNGT] # 75 255 # # TspEI: # [AATT] # 7 31 292 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 330 # Reported_sequences: 1 # Reported_hitcount: 87 #---------------------------------------