Restriction Fragments for GUT2/YIL155C



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The currently selected gene/sequence is :

GUT2/YIL155C on chromosome IX from coordinates 53708 to 51759.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.21141.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 10 14 + MaeIII GTNAC 9 14 . . 17 21 + MboII GAAGA 29 28 . . 20 24 + MboII GAAGA 32 31 . . 24 27 + SetI ASST 27 23 . . 24 27 + CviJI RGCY 25 25 . . 24 27 + AluI AGCT 25 25 . . 25 35 + MwoI GCNNNNNNNGC 31 28 . . 20 25 - Ksp632I* CTCTTC 15 18 . . 25 29 + BlsI GCNGC 27 26 . . 25 29 + BisI GCNGC 26 27 . . 25 29 + TseI GCWGC 25 28 . . 20 26 - SapI GCTCTTC 15 18 . . 28 38 + MwoI GCNNNNNNNGC 34 31 . . 28 33 + Cfr10I RCCGGY 28 32 . . 29 32 + HpaII CCGG 29 31 . . 25 29 - BbvI GCAGC 12 16 . . 32 37 + BsgI GTGCAG 53 51 . . 33 36 + CviRI* TGCA 34 34 . . 34 44 + MwoI GCNNNNNNNGC 40 37 . . 34 44 + BstAPI GCANNNNNTGC 40 37 . . 34 38 + BlsI GCNGC 36 35 . . 34 38 + BisI GCNGC 35 36 . . 34 38 + BbvI GCAGC 46 50 . . 34 38 + TseI GCWGC 34 37 . . 35 40 + PvuII CAGCTG 37 37 . . 35 40 + NspBII* CMGCKG 37 37 . . 35 43 + AlwNI CAGNNNCTG 40 37 . . 36 39 + SetI ASST 39 35 . . 36 39 + CviJI RGCY 37 37 . . 36 39 + AluI AGCT 37 37 . . 37 41 + BlsI GCNGC 39 38 . . 37 41 + BisI GCNGC 38 39 . . 37 41 + TseI GCWGC 37 40 . . 40 50 + MwoI GCNNNNNNNGC 46 43 . . 40 44 + BlsI GCNGC 42 41 . . 40 44 + BisI GCNGC 41 42 . . 40 44 + TseI GCWGC 40 43 . . 37 41 - BbvI GCAGC 24 28 . . 38 43 - EcoP15I CAGCAG 10 12 . . 44 49 + DsaI* CCRYGG 44 48 . . 44 49 + StyI CCWWGG 44 48 . . 44 49 + NcoI CCATGG 44 48 . . 44 49 + SecI* CCNNGG 44 48 . . 40 44 - BbvI GCAGC 27 31 . . 45 48 + FatI CATG 44 48 . . 45 48 + NlaIII CATG 48 44 . . 45 48 + CviAII CATG 45 47 . . 47 52 + BalI TGGCCA 49 49 . . 47 52 + CfrI YGGCCR 47 51 . . 48 51 + CviJI RGCY 49 49 . . 48 51 + HaeIII GGCC 49 49 . . 54 57 + CviJI RGCY 55 55 . . 56 61 + DsaI* CCRYGG 56 60 . . 56 61 + SecI* CCNNGG 56 60 . . 61 65 + BslFI GGGAC 75 79 . . 63 67 + HgaI GACGC 72 77 . . 68 73 + TatI WGTACW 68 72 . . 69 72 + Csp6I GTAC 69 71 . . 69 72 + RsaI GTAC 70 70 . . 71 75 + BsrI ACTGG 76 74 . . 74 78 + FokI GGATG 87 91 . . 74 78 + BseGI GGATG 80 78 . . 80 83 + MaeI CTAG 80 82 . . 81 86 + TsoI TARCCA 97 95 . . 82 85 + CviJI RGCY 83 83 . . 84 89 + StyI CCWWGG 84 88 . . 84 89 + SecI* CCNNGG 84 88 . . 87 90 + SetI ASST 90 86 . . 88 92 + HphI GGTGA 100 99 . . 95 98 + CviJI RGCY 96 96 . . 95 98 + HaeIII GGCC 96 96 . . 96 106 + MwoI GCNNNNNNNGC 102 99 . . 96 100 - BceAI ACGGC 81 83 . . 103 108 + Hpy166II GTNNAC 105 105 . . 103 108 + SduI GDGCHC 107 103 . . 103 108 + BseSI GKGCMC 107 103 . . 103 108 + ApaLI GTGCAC 103 107 . . 103 108 + HgiAI* GWGCWC 107 103 . . 104 107 + CviRI* TGCA 105 105 . . 114 119 + BmeT110I CYCGRG 115 117 . . 114 119 + AvaI CYCGRG 114 118 . . 118 121 + SetI ASST 121 117 . . 118 121 + CviJI RGCY 119 119 . . 118 121 + AluI AGCT 119 119 . . 123 126 + FatI CATG 122 126 . . 123 126 + NlaIII CATG 126 122 . . 123 126 + CviAII CATG 123 125 . . 128 131 + CviRI* TGCA 129 129 . . 131 134 + TspEI AATT 130 134 . . 138 149 + TstI CACNNNNNNTCC 129 124 161 156 139 149 + BsaXI ACNNNNNCTCC 129 126 159 156 140 143 + AciI CCGC 140 142 . . 142 146 + TauI GCSGC 145 142 . . 142 146 + BlsI GCNGC 144 143 . . 142 146 + BisI GCNGC 143 144 . . 143 146 + AciI CCGC 143 145 . . 143 148 + BsrBI CCGCTC 145 145 . . 151 154 + AciI CCGC 151 153 . . 155 158 + SetI ASST 158 154 . . 156 161 + Eco31I GGTCTC 162 166 . . 157 161 + BsmAI GTCTC 162 166 . . 153 158 - BspMI ACCTGC 144 148 . . 160 165 + XbaI TCTAGA 160 164 . . 160 165 + Hpy178III* TCNNGA 161 163 . . 161 164 + MaeI CTAG 161 163 . . 170 173 + SetI ASST 173 169 . . 170 175 + BspMI ACCTGC 179 183 . . 167 171 - BsmAI GTCTC 161 165 . . 167 172 - Eco31I GGTCTC 161 165 . . 172 177 - EcoP15I CAGCAG 144 146 . . 177 181 + BmgT120I GGNCC 178 179 . . 177 181 + AvaII GGWCC 177 180 . . 177 181 + AsuI* GGNCC 177 180 . . 179 183 + Tsp4CI* ACNGT 181 180 . . 185 190 + BalI TGGCCA 187 187 . . 185 190 + CfrI YGGCCR 185 189 . . 186 189 + CviJI RGCY 187 187 . . 186 189 + HaeIII GGCC 187 187 . . 192 196 + HgaI GACGC 201 206 . . 195 199 + SfaNI GCATC 204 208 . . 200 203 + TspEI AATT 199 203 . . 203 206 + TaqI TCGA 203 205 . . 204 208 + Hpy99I CGWCG 208 203 . . 206 209 + TaiI ACGT 209 205 . . 206 209 + SetI ASST 209 205 . . 206 209 + MaeII ACGT 206 208 . . 206 211 + AflIII ACRYGT 206 210 . . 205 210 - BtrI CACGTC 207 207 . . 212 217 + BclI TGATCA 212 216 . . 213 216 + DpnI GATC 214 214 . . 213 216 + BstKTI GATC 215 213 . . 213 216 + MboI GATC 212 216 . . 226 231 + NlaIV GGNNCC 228 228 . . 227 231 + BmgT120I GGNCC 228 229 . . 227 231 + AsuI* GGNCC 227 230 . . 224 227 - AciI CCGC 224 226 . . 224 228 - FauI CCCGC 217 219 . . 228 231 + CviJI RGCY 229 229 . . 228 231 + HaeIII GGCC 229 229 . . 230 235 + DsaI* CCRYGG 230 234 . . 230 235 + SecI* CCNNGG 230 234 . . 235 239 + BslFI GGGAC 249 253 . . 241 245 + FokI GGATG 254 258 . . 241 245 + BseGI GGATG 247 245 . . 245 250 + SduI GDGCHC 249 245 . . 245 250 + BseSI GKGCMC 249 245 . . 236 247 - TstI CACNNNNNNTCC 260 255 228 223 247 257 + MwoI GCNNNNNNNGC 253 250 . . 250 253 + MaeI CTAG 250 252 . . 256 260 + BlsI GCNGC 258 257 . . 256 260 + BisI GCNGC 257 258 . . 256 260 + TseI GCWGC 256 259 . . 253 257 - SfaNI GCATC 243 247 . . 256 260 - BbvI GCAGC 243 247 . . 263 267 + BssKI CCNGG 262 267 . . 263 267 + BseBI CCWGG 264 265 . . 263 268 + SecI* CCNNGG 263 267 . . 263 267 + ScrFI CCNGG 264 265 . . 263 267 + EcoRII CCWGG 262 267 . . 267 271 + BslFI GGGAC 281 285 . . 269 273 + HinfI GANTC 269 272 . . 269 273 - PleI GAGTC 263 264 . . 269 273 - MlyI GAGTC 263 263 . . 268 279 - TstI CACNNNNNNTCC 292 287 260 255 279 282 + CviJI RGCY 280 280 . . 279 282 + HaeIII GGCC 280 280 . . 279 283 + BmgT120I GGNCC 280 281 . . 279 283 + AsuI* GGNCC 279 282 . . 298 308 + Hin4I GAYNNNNNVTC 289 284 321 316 305 308 + MnlI CCTC 315 314 . . 305 310 + SecI* CCNNGG 305 309 . . 306 311 + BmeT110I CYCGRG 307 309 . . 306 311 + AvaI CYCGRG 306 310 . . 307 312 + Hpy178III* TCNNGA 308 310 . . 313 316 + TaiI ACGT 316 312 . . 313 316 + SetI ASST 316 312 . . 313 316 + MaeII ACGT 313 315 . . 314 318 + Hpy99I CGWCG 318 313 . . 336 340 + TfiI GAWTC 336 339 . . 336 340 + HinfI GANTC 336 339 . . 341 345 + Tsp4CI* ACNGT 343 342 . . 354 357 + Csp6I GTAC 354 356 . . 354 357 + RsaI GTAC 355 355 . . 351 354 - AciI CCGC 351 353 . . 357 361 + DdeI CTNAG 357 360 . . 363 372 + XmnI GAANNNNTTC 367 367 . . 365 370 + StuI AGGCCT 367 367 . . 366 369 + CviJI RGCY 367 367 . . 366 369 + HaeIII GGCC 367 367 . . 363 367 - Hin4II* CCTTC 357 356 . . 368 372 + Hin4II* CCTTC 378 377 . . 383 397 + XcmI CCANNNNNNNNNTGG 390 389 . . 383 388 + StyI CCWWGG 383 387 . . 383 388 + SecI* CCNNGG 383 387 . . 393 397 + BsrI ACTGG 398 396 . . 396 401 + XhoII RGATCY 396 400 . . 396 400 + BinI* GGATC 404 405 . . 397 400 + DpnI GATC 398 398 . . 397 400 + BstKTI GATC 399 397 . . 397 400 + MboI GATC 396 400 . . 407 410 + TaqI TCGA 407 409 . . 409 412 - MnlI CCTC 402 401 . . 425 430 + Hpy166II GTNNAC 427 427 . . 441 447 + TspRI CASTGNN 447 438 . . 441 446 - BtsI GCAGTG 440 438 . . 448 451 + MnlI CCTC 458 457 . . 452 455 + SetI ASST 455 451 . . 453 463 + BsiYI* CCNNNNNNNGG 459 456 . . 450 454 - HphI GGTGA 442 441 . . 456 461 + Hpy166II GTNNAC 458 458 . . 456 461 + SduI GDGCHC 460 456 . . 456 461 + BseSI GKGCMC 460 456 . . 456 461 + ApaLI GTGCAC 456 460 . . 456 461 + HgiAI* GWGCWC 460 456 . . 457 460 + CviRI* TGCA 458 458 . . 460 464 + Tsp4CI* ACNGT 462 461 . . 471 474 + TspEI AATT 470 474 . . 474 478 + Hpy188I TCNGA 476 475 . . 477 480 + DpnI GATC 478 478 . . 477 480 + BstKTI GATC 479 477 . . 477 486 + BsaBI GATNNNNATC 481 481 . . 477 480 + MboI GATC 476 480 . . 477 481 - BinI* GGATC 471 472 . . 482 486 + BccI CCATC 490 491 . . 486 491 + SfeI* CTRYAG 486 490 . . 489 497 + AlwNI CAGNNNCTG 494 491 . . 493 496 + SetI ASST 496 492 . . 494 498 + BssKI CCNGG 493 498 . . 494 498 + BseBI CCWGG 495 496 . . 494 498 + ScrFI CCNGG 495 496 . . 494 498 + EcoRII CCWGG 493 498 . . 500 503 + SetI ASST 503 499 . . 500 506 + PpuMI RGGWCCY 501 504 . . 500 506 + DraII RGGNCCY 501 504 . . 501 506 + NlaIV GGNNCC 503 503 . . 501 505 + BmgT120I GGNCC 502 503 . . 501 505 + AvaII GGWCC 501 504 . . 501 505 + AsuI* GGNCC 501 504 . . 498 503 - BspMI ACCTGC 489 493 . . 502 506 - BslFI GGGAC 487 491 . . 507 510 + Csp6I GTAC 507 509 . . 507 510 + RsaI GTAC 508 508 . . 510 519 + MslI CAYNNNNRTG 514 514 . . 520 523 + CviJI RGCY 521 521 . . 526 531 + ApoI RAATTY 526 530 . . 527 530 + TspEI AATT 526 530 . . 534 545 + BcgI CGANNNNNNTGC 523 521 557 555 544 549 + Cfr10I RCCGGY 544 548 . . 545 548 + HpaII CCGG 545 547 . . 547 552 + NlaIV GGNNCC 549 549 . . 572 575 + SetI ASST 575 571 . . 576 580 + Tsp4CI* ACNGT 578 577 . . 587 590 + AciI CCGC 587 589 . . 586 591 - EciI GGCGGA 576 574 . . 592 596 + Tsp4CI* ACNGT 594 593 . . 593 598 + DsaI* CCRYGG 593 597 . . 593 598 + SecI* CCNNGG 593 597 . . 603 606 + CviJI RGCY 604 604 . . 603 608 + NlaIV GGNNCC 605 605 . . 600 604 - Hin4II* CCTTC 594 593 . . 609 612 + FatI CATG 608 612 . . 609 612 + NlaIII CATG 612 608 . . 609 612 + CviAII CATG 609 611 . . 625 628 + TspEI AATT 624 628 . . 627 632 + AhaIII* TTTAAA 629 629 . . 628 631 + MseI TTAA 628 630 . . 632 637 + StuI AGGCCT 634 634 . . 633 636 + CviJI RGCY 634 634 . . 633 636 + HaeIII GGCC 634 634 . . 635 638 + MnlI CCTC 645 644 . . 643 648 + Hpy166II GTNNAC 645 645 . . 645 648 + Csp6I GTAC 645 647 . . 645 648 + RsaI GTAC 646 646 . . 649 652 + FatI CATG 648 652 . . 649 652 + NlaIII CATG 652 648 . . 649 652 + CviAII CATG 649 651 . . 655 661 + PpuMI RGGWCCY 656 659 . . 655 661 + DraII RGGNCCY 656 659 . . 655 660 + NlaIV GGNNCC 657 657 . . 656 660 + BmgT120I GGNCC 657 658 . . 652 656 - BccI CCATC 646 647 . . 656 660 + AvaII GGWCC 656 659 . . 656 660 + AsuI* GGNCC 656 659 . . 662 665 + MseI TTAA 662 664 . . 667 671 + HinfI GANTC 667 670 . . 667 671 - PleI GAGTC 661 662 . . 667 671 - MlyI GAGTC 661 661 . . 671 674 + FnuDII* CGCG 672 672 . . 682 692 + MwoI GCNNNNNNNGC 688 685 . . 689 694 + TsoI TARCCA 705 703 . . 690 693 + CviJI RGCY 691 691 . . 691 701 + MwoI GCNNNNNNNGC 697 694 . . 691 701 + BglI GCCNNNNNGGC 697 694 . . 692 706 + XcmI CCANNNNNNNNNTGG 699 698 . . 692 696 + BccI CCATC 700 701 . . 696 705 + MslI CAYNNNNRTG 700 700 . . 696 705 + OliI CACNNNNGTG 700 700 . . 697 701 + BceAI ACGGC 713 715 . . 699 702 + CviJI RGCY 700 700 . . 710 714 + BceAI ACGGC 726 728 . . 712 717 + HaeII RGCGCY 716 712 . . 713 716 + Hin6I GCGC 713 715 . . 713 716 + GlaI GCGC 714 714 . . 713 716 + HhaI GCGC 715 713 . . 718 722 + Tsp4CI* ACNGT 720 719 . . 722 727 + Hpy178III* TCNNGA 723 725 . . 734 737 + TaqI TCGA 734 736 . . 737 740 + SetI ASST 740 736 . . 736 739 - MnlI CCTC 729 728 . . 739 742 + Csp6I GTAC 739 741 . . 739 742 + RsaI GTAC 740 740 . . 746 749 + TspEI AATT 745 749 . . 749 754 + BclI TGATCA 749 753 . . 750 753 + DpnI GATC 751 751 . . 750 753 + BstKTI GATC 752 750 . . 750 753 + MboI GATC 749 753 . . 760 770 + BsiYI* CCNNNNNNNGG 766 763 . . 760 770 + PflMI CCANNNNNTGG 766 763 . . 773 776 + SetI ASST 776 772 . . 781 786 + NlaIV GGNNCC 783 783 . . 781 786 + HgiCI* GGYRCC 781 785 . . 784 789 + NmeAIII GCCGAG 810 808 . . 785 790 + SecI* CCNNGG 785 789 . . 785 795 + BsiYI* CCNNNNNNNGG 791 788 . . 789 792 + CviJI RGCY 790 790 . . 789 792 + HaeIII GGCC 790 790 . . 789 793 + BmgT120I GGNCC 790 791 . . 789 793 + AsuI* GGNCC 789 792 . . 787 790 - MnlI CCTC 780 779 . . 791 796 + SmaI CCCGGG 793 793 . . 791 796 + Cfr9I CCCGGG 791 795 . . 791 795 + BssKI CCNGG 790 795 . . 791 796 + SecI* CCNNGG 791 795 . . 791 796 + BmeT110I CYCGRG 792 794 . . 791 795 + ScrFI CCNGG 792 793 . . 791 795 + CauII* CCSGG 792 793 . . 791 796 + AvaI CYCGRG 791 795 . . 792 796 + BssKI CCNGG 791 796 . . 792 795 + HpaII CCGG 792 794 . . 792 796 + ScrFI CCNGG 793 794 . . 792 796 + CauII* CCSGG 793 794 . . 794 798 + BslFI GGGAC 808 812 . . 797 800 + TaiI ACGT 800 796 . . 797 800 + SetI ASST 800 796 . . 797 800 + MaeII ACGT 797 799 . . 802 806 - BsmAI GTCTC 796 800 . . 812 815 + SetI ASST 815 811 . . 812 815 + CviJI RGCY 813 813 . . 812 815 + AluI AGCT 813 813 . . 818 822 + Hpy188I TCNGA 820 819 . . 821 825 + TfiI GAWTC 821 824 . . 821 825 + HinfI GANTC 821 824 . . 848 853 + DsaI* CCRYGG 848 852 . . 848 853 + SecI* CCNNGG 848 852 . . 852 857 + SduI GDGCHC 856 852 . . 852 857 + Bsp120I GGGCCC 852 856 . . 852 857 + NlaIV GGNNCC 854 854 . . 852 856 + BmgT120I GGNCC 853 854 . . 852 857 + HgiJII* GRGCYC 856 852 . . 852 857 + BseSI GKGCMC 856 852 . . 852 856 + AsuI* GGNCC 852 855 . . 852 857 + ApaI GGGCCC 856 852 . . 853 856 + CviJI RGCY 854 854 . . 853 856 + HaeIII GGCC 854 854 . . 853 857 + BmgT120I GGNCC 854 855 . . 853 857 + AsuI* GGNCC 853 856 . . 860 864 + Tsp4CI* ACNGT 862 861 . . 861 867 + TspRI CASTGNN 867 858 . . 863 867 + Tsp45I GTSAC 862 867 . . 863 867 + MaeIII GTNAC 862 867 . . 865 869 + HgaI GACGC 874 879 . . 865 870 + AcyI GRCGYC 866 868 . . 869 883 + XcmI CCANNNNNNNNNTGG 876 875 . . 875 878 + CviRI* TGCA 876 876 . . 882 886 + BmgT120I GGNCC 883 884 . . 882 886 + AvaII GGWCC 882 885 . . 882 886 + AsuI* GGNCC 882 885 . . 885 888 + AciI CCGC 885 887 . . 892 896 + BccI CCATC 900 901 . . 895 900 + BetI* WCCGGW 895 899 . . 896 899 + HpaII CCGG 896 898 . . 893 897 - FokI GGATG 879 883 . . 893 897 - BseGI GGATG 892 890 . . 904 907 + HpaII CCGG 904 906 . . 907 911 + HinfI GANTC 907 910 . . 907 911 - PleI GAGTC 901 902 . . 907 911 - MlyI GAGTC 901 901 . . 912 916 + FauI CCCGC 920 922 . . 913 916 + AciI CCGC 913 915 . . 920 930 + BsaXI ACNNNNNCTCC 910 907 940 937 933 936 + DpnI GATC 934 934 . . 933 936 + BstKTI GATC 935 933 . . 933 936 + MboI GATC 932 936 . . 939 944 + SalI GTCGAC 939 943 . . 939 944 + Hpy166II GTNNAC 941 941 . . 939 944 + HindII GTYRAC 941 941 . . 939 944 + AccI GTMKAC 940 942 . . 940 943 + TaqI TCGA 940 942 . . 942 952 + Hin4I GAYNNNNNVTC 933 928 965 960 942 952 - Hin4I GAYNNNNNVTC 965 960 933 928 958 962 - BceAI ACGGC 943 945 . . 963 966 + FatI CATG 962 966 . . 963 966 + NlaIII CATG 966 962 . . 963 966 + CviAII CATG 963 965 . . 966 971 + NlaIV GGNNCC 968 968 . . 966 970 + BmgT120I GGNCC 967 968 . . 966 970 + AvaII GGWCC 966 969 . . 966 970 + AsuI* GGNCC 966 969 . . 969 979 + BsiYI* CCNNNNNNNGG 975 972 . . 981 985 - FokI GGATG 967 971 . . 981 985 - BseGI GGATG 980 978 . . 985 989 + BccI CCATC 993 994 . . 985 995 + BsiYI* CCNNNNNNNGG 991 988 . . 985 995 + PflMI CCANNNNNTGG 991 988 . . 997 1002 + Hpy166II GTNNAC 999 999 . . 1013 1016 + MnlI CCTC 1023 1022 . . 1029 1033 - Hin4II* CCTTC 1023 1022 . . 1048 1053 + ZraI GACGTC 1050 1050 . . 1048 1053 + AcyI GRCGYC 1049 1051 . . 1048 1053 + AatII GACGTC 1052 1048 . . 1049 1052 + TaiI ACGT 1052 1048 . . 1049 1052 + SetI ASST 1052 1048 . . 1044 1049 - MmeI TCCRAC 1025 1023 . . 1049 1052 + MaeII ACGT 1049 1051 . . 1052 1056 + Hpy188I TCNGA 1054 1053 . . 1057 1060 + SetI ASST 1060 1056 . . 1058 1061 + MnlI CCTC 1068 1067 . . 1060 1064 + Hpy188I TCNGA 1062 1061 . . 1063 1067 - BccI CCATC 1057 1058 . . 1086 1089 + SetI ASST 1089 1085 . . 1087 1092 + StyI CCWWGG 1087 1091 . . 1087 1092 + SecI* CCNNGG 1087 1091 . . 1087 1097 + BsiYI* CCNNNNNNNGG 1093 1090 . . 1108 1113 + NaeI GCCGGC 1110 1110 . . 1108 1113 + MroNI GCCGGC 1108 1112 . . 1108 1113 + Cac8I GCNNGC 1110 1110 . . 1108 1113 + Cfr10I RCCGGY 1108 1112 . . 1109 1112 + HpaII CCGG 1109 1111 . . 1111 1116 + NlaIV GGNNCC 1113 1113 . . 1111 1116 + HgiCI* GGYRCC 1111 1115 . . 1122 1126 - FokI GGATG 1108 1112 . . 1122 1126 - BseGI GGATG 1121 1119 . . 1138 1142 - BslFI GGGAC 1123 1127 . . 1157 1162 + SfeI* CTRYAG 1157 1161 . . 1160 1168 + AlwNI CAGNNNCTG 1165 1162 . . 1164 1167 + CviJI RGCY 1165 1165 . . 1162 1165 - MnlI CCTC 1155 1154 . . 1173 1178 + Hpy178III* TCNNGA 1174 1176 . . 1177 1182 + EcoRV GATATC 1179 1179 . . 1181 1186 + Hpy178III* TCNNGA 1182 1184 . . 1192 1197 + SfeI* CTRYAG 1192 1196 . . 1205 1208 + TaqI TCGA 1205 1207 . . 1207 1212 + EcoRI GAATTC 1207 1211 . . 1207 1212 + ApoI RAATTY 1207 1211 . . 1208 1211 + TspEI AATT 1207 1211 . . 1225 1229 + MboII GAAGA 1237 1236 . . 1225 1230 + BbvII* GAAGAC 1232 1236 . . 1229 1232 + TaiI ACGT 1232 1228 . . 1229 1232 + SetI ASST 1232 1228 . . 1229 1232 + MaeII ACGT 1229 1231 . . 1228 1233 - BtrI CACGTC 1230 1230 . . 1234 1238 + DdeI CTNAG 1234 1237 . . 1239 1242 + CviRI* TGCA 1240 1240 . . 1241 1244 + FatI CATG 1240 1244 . . 1241 1244 + NlaIII CATG 1244 1240 . . 1241 1244 + CviAII CATG 1241 1243 . . 1245 1248 + CviJI RGCY 1246 1246 . . 1253 1257 + Hpy188I TCNGA 1255 1254 . . 1256 1266 + Hin4I GAYNNNNNVTC 1247 1242 1279 1274 1256 1266 + Eam1105I GACNNNNNGTC 1261 1260 . . 1257 1260 + SetI ASST 1260 1256 . . 1265 1269 + Hpy188I TCNGA 1267 1266 . . 1256 1266 - Hin4I GAYNNNNNVTC 1279 1274 1247 1242 1269 1274 + XhoII RGATCY 1269 1273 . . 1270 1273 + DpnI GATC 1271 1271 . . 1270 1273 + BstKTI GATC 1272 1270 . . 1270 1273 + MboI GATC 1269 1273 . . 1274 1285 + TstI CACNNNNNNTCC 1265 1260 1297 1292 1274 1279 + BsaAI YACGTR 1276 1276 . . 1270 1274 - BinI* GGATC 1264 1265 . . 1275 1278 + TaiI ACGT 1278 1274 . . 1275 1278 + SetI ASST 1278 1274 . . 1275 1278 + MaeII ACGT 1275 1277 . . 1277 1280 + Csp6I GTAC 1277 1279 . . 1277 1280 + RsaI GTAC 1278 1278 . . 1285 1289 + FauI CCCGC 1293 1295 . . 1285 1295 + BsiYI* CCNNNNNNNGG 1291 1288 . . 1286 1296 + BsiYI* CCNNNNNNNGG 1292 1289 . . 1286 1289 + AciI CCGC 1286 1288 . . 1296 1300 + MboII GAAGA 1308 1307 . . 1302 1307 + SduI GDGCHC 1306 1302 . . 1302 1307 + HgiJII* GRGCYC 1306 1302 . . 1299 1303 - Hin4II* CCTTC 1293 1292 . . 1303 1306 + CviJI RGCY 1304 1304 . . 1310 1324 + XcmI CCANNNNNNNNNTGG 1317 1316 . . 1313 1317 + DdeI CTNAG 1313 1316 . . 1313 1317 + BspCNI CTCAG 1326 1324 . . 1313 1317 + BseMII CTCAG 1327 1325 . . 1327 1332 + XhoII RGATCY 1327 1331 . . 1328 1331 + DpnI GATC 1329 1329 . . 1328 1331 + BstKTI GATC 1330 1328 . . 1328 1331 + MboI GATC 1327 1331 . . 1328 1332 - BinI* GGATC 1322 1323 . . 1341 1346 + Hpy166II GTNNAC 1343 1343 . . 1348 1352 + Hpy188I TCNGA 1350 1349 . . 1357 1360 + CviJI RGCY 1358 1358 . . 1357 1360 + HaeIII GGCC 1358 1358 . . 1362 1365 + TspEI AATT 1361 1365 . . 1371 1374 + CviRI* TGCA 1372 1372 . . 1374 1377 + SetI ASST 1377 1373 . . 1372 1377 - BspMI ACCTGC 1363 1367 . . 1372 1378 - AarI CACCTGC 1363 1367 . . 1404 1407 + CviJI RGCY 1405 1405 . . 1406 1410 + DdeI CTNAG 1406 1409 . . 1406 1410 - BspCNI CTCAG 1398 1396 . . 1406 1410 - BseMII CTCAG 1397 1395 . . 1408 1411 - MnlI CCTC 1401 1400 . . 1405 1411 - Bpu10I CCTNAGC 1406 1409 . . 1405 1411 - BbvCI CCTCAGC 1406 1409 . . 1414 1418 + Tsp4CI* ACNGT 1416 1415 . . 1417 1422 + SalI GTCGAC 1417 1421 . . 1417 1422 + Hpy166II GTNNAC 1419 1419 . . 1417 1422 + HindII GTYRAC 1419 1419 . . 1417 1422 + AccI GTMKAC 1418 1420 . . 1418 1421 + TaqI TCGA 1418 1420 . . 1420 1431 + DrdI GACNNNNNNGTC 1426 1424 . . 1430 1433 + TaqI TCGA 1430 1432 . . 1438 1443 + EciI GGCGGA 1454 1452 . . 1442 1446 + TfiI GAWTC 1442 1445 . . 1442 1446 + HinfI GANTC 1442 1445 . . 1439 1442 - AciI CCGC 1439 1441 . . 1451 1454 + SetI ASST 1454 1450 . . 1458 1461 + SetI ASST 1461 1457 . . 1463 1467 + Tsp45I GTSAC 1462 1467 . . 1463 1467 + MaeIII GTNAC 1462 1467 . . 1478 1481 + MseI TTAA 1478 1480 . . 1480 1485 + HindIII AAGCTT 1480 1484 . . 1481 1484 + SetI ASST 1484 1480 . . 1481 1484 + CviJI RGCY 1482 1482 . . 1481 1484 + AluI AGCT 1482 1482 . . 1482 1487 + Cac8I GCNNGC 1484 1484 . . 1490 1495 + BsgI GTGCAG 1511 1509 . . 1491 1494 + CviRI* TGCA 1492 1492 . . 1495 1499 + MboII GAAGA 1507 1506 . . 1503 1507 + HgaI GACGC 1512 1517 . . 1518 1521 + CviJI RGCY 1519 1519 . . 1519 1529 + MwoI GCNNNNNNNGC 1525 1522 . . 1527 1530 + CviJI RGCY 1528 1528 . . 1558 1563 + MmeI TCCRAC 1583 1581 . . 1559 1570 + BstXI CCANNNNNNTGG 1566 1562 . . 1580 1584 + TspGWI ACGGA 1595 1593 . . 1582 1587 + NlaIV GGNNCC 1584 1584 . . 1583 1592 + XmnI GAANNNNTTC 1587 1587 . . 1592 1595 + MnlI CCTC 1602 1601 . . 1606 1611 + ApoI RAATTY 1606 1610 . . 1607 1610 + TspEI AATT 1606 1610 . . 1618 1622 + TfiI GAWTC 1618 1621 . . 1618 1622 + HinfI GANTC 1618 1621 . . 1622 1627 + DsaI* CCRYGG 1622 1626 . . 1622 1627 + StyI CCWWGG 1622 1626 . . 1622 1627 + NcoI CCATGG 1622 1626 . . 1622 1627 + SecI* CCNNGG 1622 1626 . . 1623 1626 + FatI CATG 1622 1626 . . 1623 1626 + NlaIII CATG 1626 1622 . . 1623 1626 + CviAII CATG 1623 1625 . . 1646 1652 + Bpu10I CCTNAGC 1647 1650 . . 1647 1651 + DdeI CTNAG 1647 1650 . . 1650 1653 + CviJI RGCY 1651 1651 . . 1667 1670 + TaiI ACGT 1670 1666 . . 1667 1670 + SetI ASST 1670 1666 . . 1667 1670 + MaeII ACGT 1667 1669 . . 1679 1682 + MaeI CTAG 1679 1681 . . 1684 1689 + BseRI GAGGAG 1699 1697 . . 1684 1687 - MnlI CCTC 1677 1676 . . 1702 1705 + TspEI AATT 1701 1705 . . 1715 1719 + Hpy188I TCNGA 1717 1716 . . 1718 1723 + EcoRV GATATC 1720 1720 . . 1735 1739 + HphI GGTGA 1747 1746 . . 1741 1744 + MseI TTAA 1741 1743 . . 1752 1755 + FatI CATG 1751 1755 . . 1752 1755 + NlaIII CATG 1755 1751 . . 1752 1755 + CviAII CATG 1752 1754 . . 1754 1757 + CviRI* TGCA 1755 1755 . . 1758 1761 + Csp6I GTAC 1758 1760 . . 1758 1761 + RsaI GTAC 1759 1759 . . 1763 1768 + SspI AATATT 1765 1765 . . 1778 1783 + StyI CCWWGG 1778 1782 . . 1778 1783 + SecI* CCNNGG 1778 1782 . . 1792 1795 + MseI TTAA 1792 1794 . . 1796 1800 + MboII GAAGA 1808 1807 . . 1805 1809 + TfiI GAWTC 1805 1808 . . 1805 1809 + HinfI GANTC 1805 1808 . . 1811 1815 + Hin4II* CCTTC 1821 1820 . . 1819 1823 + HgaI GACGC 1828 1833 . . 1819 1824 + AcyI GRCGYC 1820 1822 . . 1822 1832 + MwoI GCNNNNNNNGC 1828 1825 . . 1823 1828 + StyI CCWWGG 1823 1827 . . 1823 1828 + SecI* CCNNGG 1823 1827 . . 1829 1834 + HindIII AAGCTT 1829 1833 . . 1830 1833 + SetI ASST 1833 1829 . . 1830 1833 + CviJI RGCY 1831 1831 . . 1830 1833 + AluI AGCT 1831 1831 . . 1831 1841 + MwoI GCNNNNNNNGC 1837 1834 . . 1836 1841 + BsmI GAATGC 1842 1840 . . 1840 1850 + MwoI GCNNNNNNNGC 1846 1843 . . 1844 1847 + CviRI* TGCA 1845 1845 . . 1840 1844 - BceAI ACGGC 1825 1827 . . 1845 1850 + NspI RCATGY 1849 1845 . . 1845 1850 + Cac8I GCNNGC 1847 1847 . . 1845 1850 + SphI GCATGC 1849 1845 . . 1846 1849 + FatI CATG 1845 1849 . . 1846 1849 + NlaIII CATG 1849 1845 . . 1846 1849 + CviAII CATG 1846 1848 . . 1852 1856 + Tsp4CI* ACNGT 1854 1853 . . 1867 1871 + HphI GGTGA 1879 1878 . . 1865 1870 - TaqII CACCCA 1855 1853 . . 1878 1883 + MfeI CAATTG 1878 1882 . . 1879 1882 + TspEI AATT 1878 1882 . . 1885 1889 + Hpy188I TCNGA 1887 1886 . . 1884 1889 - MmeI TCCRAC 1865 1863 . . 1896 1899 - MnlI CCTC 1889 1888 . . 1899 1904 + BtsI GCAGTG 1906 1904 . . 1900 1906 + TspRI CASTGNN 1906 1897 . . 1918 1922 + Tsp4CI* ACNGT 1920 1919 . . 1921 1926 + Hpy166II GTNNAC 1923 1923 . . 1922 1933 + BdaI TGANNNNNNTCA 1911 1909 1945 1943 1927 1931 - TspDTI ATGAA 1917 1915 . . 1931 1936 + Hpy178III* TCNNGA 1932 1934 . . 1922 1933 - BdaI TGANNNNNNTCA 1945 1943 1911 1909 1936 1939 + TaiI ACGT 1939 1935 . . 1936 1939 + SetI ASST 1939 1935 . . 1936 1939 + MaeII ACGT 1936 1938 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 587 1363 # # AatII: # [GACGTC] # 898 1052 # # AccI: # [GTMKAC] # 478 532 940 # # AciI: # [CCGC] # 3 8 28 73 127 140 # 153 236 298 373 511 # # AcyI: # [GRCGYC] # 130 183 771 866 # # AflIII: # [ACRYGT] # 206 1744 # # AhaIII*: # [TTTAAA] # 629 1321 # # AluI: # [AGCT] # 12 25 82 119 349 669 # 694 # # AlwNI: # [CAGNNNCTG] # 40 454 671 785 # # ApaI: # [GGGCCC] # 856 1094 # # ApaLI: # [GTGCAC] # 103 353 1494 # # ApoI: # [RAATTY] # 344 399 526 681 # # AsuI*: # [GGNCC] # 1 29 50 52 63 84 # 133 155 177 222 984 # # AvaI: # [CYCGRG] # 114 192 485 1159 # # AvaII: # [GGWCC] # 84 155 177 226 324 984 # # BalI: # [TGGCCA] # 49 138 1763 # # BbvCI: # [CCTCAGC] # 544 1406 # # BbvI: # [GCAGC] # 3 12 12 19 197 1707 # # BbvII*: # [GAAGAC] # 718 1232 # # BccI: # [CCATC] # 54 64 93 156 200 490 # 893 # # BceAI: # [ACGGC] # 13 81 125 217 632 882 # # BcgI: # [CGANNNNNNTGC] # 34 523 1393 # # BclI: # [TGATCA] # 212 537 1201 # # BdaI: # [TGANNNNNNTCA] # 0 0 5 34 1911 # # BetI*: # [WCCGGW] # 895 1055 # # BglI: # [GCCNNNNNGGC] # 697 1253 # # BinI*: # [GGATC] # 58 67 404 628 793 # # BisI: # [GCNGC] # 3 3 9 26 102 114 # 1693 # # BlsI: # [GCNGC] # 3 3 9 27 102 114 # 1692 # # BmeT110I: # [CYCGRG] # 115 192 485 1158 # # BmgT120I: # [GGNCC] # 1 29 50 52 63 84 # 133 155 178 222 983 # # Bpu10I: # [CCTNAGC] # 241 303 1406 # # BsaAI: # [YACGTR] # 674 1276 # # BsaBI: # [GATNNNNATC] # 481 1469 # # BsaXI: # [ACNNNNNCTCC] # 30 30 129 751 1010 # # BseBI: # [CCWGG] # 231 264 1455 # # BseGI: # [GGATG] # 80 88 141 167 645 829 # # BseMII: # [CTCAG] # 70 553 1327 # # BseRI: # [GAGGAG] # 251 1699 # # BseSI: # [GKGCMC] # 107 142 211 396 1094 # # BsgI: # [GTGCAG] # 53 439 1458 # # BsiYI*: # [CCNNNNNNNGG] # 1 16 25 102 184 198 # 307 459 658 # # BslFI: # [GGGAC] # 32 75 174 206 315 321 # 827 # # BsmAI: # [GTCTC] # 1 161 634 1154 # # BsmI: # [GAATGC] # 108 1842 # # Bsp120I: # [GGGCCC] # 852 1098 # # BspCNI: # [CTCAG] # 72 552 1326 # # BspMI: # [ACCTGC] # 35 144 310 587 874 # # BsrBI: # [CCGCTC] # 145 1805 # # BsrI: # [ACTGG] # 76 322 1552 # # BssKI: # [CCNGG] # 1 231 262 297 1159 # # BstAPI: # [GCANNNNNTGC] # 40 1910 # # BstKTI: # [GATC] # 58 80 183 184 215 273 # 337 620 # # BstXI: # [CCANNNNNNTGG] # 384 1566 # # BtrI: # [CACGTC] # 207 720 1023 # # BtsI: # [GCAGTG] # 44 440 1466 # # Cac8I: # [GCNNGC] # 103 363 374 1110 # # CauII*: # [CCSGG] # 1 792 1157 # # Cfr10I: # [RCCGGY] # 28 516 564 842 # # Cfr9I: # [CCCGGG] # 791 1159 # # CfrI: # [YGGCCR] # 47 138 1765 # # Csp6I: # [GTAC] # 69 94 138 153 192 285 # 481 538 # # CviAII: # [CATG] # 40 45 78 94 104 129 # 278 314 382 486 # # CviJI: # [RGCY] # 6 9 9 12 12 13 # 23 23 25 28 30 37 # 41 42 47 51 54 57 # 58 68 77 81 83 87 # 90 119 123 154 180 311 # # CviRI*: # [TGCA] # 24 34 71 90 105 120 # 132 263 329 364 418 # # DdeI: # [CTNAG] # 79 93 241 303 357 877 # # DpnI: # [GATC] # 58 80 183 184 214 273 # 337 621 # # DraII: # [RGGNCCY] # 155 501 1294 # # DrdI: # [GACNNNNNNGTC] # 524 1426 # # DsaI*: # [CCRYGG] # 12 44 174 255 328 363 # 774 # # Eam1105I: # [GACNNNNNGTC] # 689 1261 # # EciI: # [GGCGGA] # 496 576 878 # # Eco31I: # [GGTCTC] # 1 161 1788 # # EcoP15I: # [CAGCAG] # 10 134 1806 # # EcoRI: # [GAATTC] # 743 1207 # # EcoRII: # [CCWGG] # 231 262 1457 # # EcoRV: # [GATATC] # 230 541 1179 # # FatI: # [CATG] # 40 44 78 94 105 129 # 278 314 382 486 # # FauI: # [CCCGC] # 217 373 657 703 # # FnuDII*: # [CGCG] # 672 1278 # # FokI: # [GGATG] # 87 88 141 167 625 842 # # GlaI: # [GCGC] # 714 1236 # # HaeII: # [RGCGCY] # 716 1234 # # HaeIII: # [GGCC] # 42 47 49 51 64 87 # 91 156 267 504 592 # # HgaI: # [GACGC] # 72 122 129 316 638 673 # # HgiAI*: # [GWGCWC] # 107 353 1490 # # HgiCI*: # [GGYRCC] # 330 781 839 # # HgiJII*: # [GRGCYC] # 450 644 856 # # HhaI: # [GCGC] # 715 1235 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 282 289 612 671 # # Hin4II*: # [CCTTC] # 21 129 216 270 357 429 # 528 # # Hin6I: # [GCGC] # 713 1237 # # HindII: # [GTYRAC] # 478 531 941 # # HindIII: # [AAGCTT] # 121 349 1480 # # HinfI: # [GANTC] # 67 86 145 154 176 187 # 269 331 535 # # HpaII: # [CCGG] # 8 29 104 205 247 516 # 841 # # HphI: # [GGTGA] # 71 100 132 342 1305 # # Hpy166II: # [GTNNAC] # 27 31 58 76 105 187 # 296 322 344 504 # # Hpy178III*: # [TCNNGA] # 8 18 147 161 415 451 # 750 # # Hpy188I: # [TCNGA] # 8 12 63 83 170 193 # 234 344 367 476 # # Hpy99I: # [CGWCG] # 110 208 1632 # # Ksp632I*: # [CTCTTC] # 15 1935 # # MaeI: # [CTAG] # 80 81 89 271 1429 # # MaeII: # [ACGT] # 14 46 107 180 206 252 # 269 392 484 # # MaeIII: # [GTNAC] # 9 488 600 853 # # MboI: # [GATC] # 58 80 183 184 212 273 # 337 623 # # MboII: # [GAAGA] # 3 29 71 142 199 301 # 1205 # # MfeI: # [CAATTG] # 72 1878 # # MlyI: # [GAGTC] # 240 263 398 1049 # # MmeI: # [TCCRAC] # 85 282 558 1025 # # MnlI: # [CCTC] # 45 51 56 61 75 84 # 87 87 187 201 212 243 # 246 315 # # MroNI: # [GCCGGC] # 842 1108 # # MseI: # [TTAA] # 34 51 158 263 628 816 # # MslI: # [CAYNNNNRTG] # 186 514 1250 # # MwoI: # [GCNNNNNNNGC] # 3 6 6 9 9 9 # 31 56 104 151 303 435 # 828 # # NaeI: # [GCCGGC] # 840 1110 # # NcoI: # [CCATGG] # 44 328 1578 # # NlaIII: # [CATG] # 40 48 78 94 101 129 # 278 314 382 486 # # NlaIV: # [GGNNCC] # 46 52 56 71 114 126 # 145 228 275 366 471 # # NmeAIII: # [GCCGAG] # 810 1140 # # NspBII*: # [CMGCKG] # 37 1913 # # NspI: # [RCATGY] # 101 1849 # # OliI: # [CACNNNNGTG] # 700 1250 # # PflMI: # [CCANNNNNTGG] # 225 766 959 # # PleI: # [GAGTC] # 240 263 398 1049 # # PpuMI: # [RGGWCCY] # 155 501 1294 # # PvuII: # [CAGCTG] # 37 1913 # # RsaI: # [GTAC] # 70 94 138 153 191 285 # 481 538 # # SalI: # [GTCGAC] # 478 533 939 # # SapI: # [GCTCTTC] # 15 1935 # # ScrFI: # [CCNGG] # 1 231 264 297 1157 # # SduI: # [GDGCHC] # 107 142 211 396 450 644 # # SecI*: # [CCNNGG] # 6 12 28 33 42 44 # 45 57 78 127 146 156 # 192 210 239 535 # # SetI: # [ASST] # 7 7 8 11 12 15 # 15 18 23 24 27 28 # 29 31 36 36 37 41 # 51 72 77 99 106 107 # 139 143 163 165 186 237 # # SfaNI: # [GCATC] # 39 204 1707 # # SfeI*: # [CTRYAG] # 35 486 671 758 # # SmaI: # [CCCGGG] # 793 1157 # # SphI: # [GCATGC] # 101 1849 # # SspI: # [AATATT] # 185 1765 # # StuI: # [AGGCCT] # 267 367 1316 # # StyI: # [CCWWGG] # 40 44 45 127 156 299 # 535 704 # # TaiI: # [ACGT] # 11 46 107 180 209 252 # 269 392 484 # # TaqI: # [TCGA] # 12 203 204 206 213 265 # 327 520 # # TaqII: # [CACCCA] # 95 1855 # # TatI: # [WGTACW] # 68 1882 # # TauI: # [GCSGC] # 145 1805 # # TfiI: # [GAWTC] # 145 176 187 336 485 621 # # TseI: # [GCWGC] # 3 3 9 25 216 1694 # # TsoI: # [TARCCA] # 97 608 1245 # # Tsp45I: # [GTSAC] # 488 600 862 # # Tsp4CI*: # [ACNGT] # 16 30 66 116 119 126 # 142 162 181 438 554 # # TspDTI: # [ATGAA] # 33 1917 # # TspEI: # [AATT] # 56 69 72 95 98 121 # 130 154 177 245 271 462 # # TspGWI: # [ACGGA] # 355 1595 # # TspRI: # [CASTGNN] # 44 420 447 1039 # # TstI: # [CACNNNNNNTCC] # 0 32 32 32 32 67 # 129 653 973 # # XbaI: # [TCTAGA] # 160 1790 # # XcmI: # [CCANNNNNNNNNTGG] # 177 309 390 441 633 # # XhoII: # [RGATCY] # 58 396 623 873 # # XmnI: # [GAANNNNTTC] # 363 367 1220 # # ZraI: # [GACGTC] # 900 1050 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1950 # Reported_sequences: 1 # Reported_hitcount: 632 #---------------------------------------